Screening of factors triggering food allergy and research on development of hypoallergic food products.

我们测试了各种环境化学物质，-六氯化苯（β - bhc）， p, p'-二氯二苯基二氯乙烯（DDE），对硝基苯2,4,6 -三基苯醚（CNP）， d -氨基苄青霉素（Amp）， o-乙酰柠檬酸三丁基酯（ATBC）对免疫反应的影响。所有这些化学物质都影响淋巴细胞对抗原反应的细胞因子分泌。这些化学物质还影响口服抗原引起的血清抗体反应。将卵白蛋白（OVA）喂给OVA特异性T细胞受体转基因小鼠，可诱导脾脏分泌白细胞介素(IL)-4细胞和血清IgE抗体反应。接下来，该系统被用于检查化学物质对过敏反应相关免疫反应的影响。发现ATBC抑制了该系统中il -4的产生。此外，我们还测试了参与脂质代谢的物质对这些T细胞受体转基因小鼠T细胞分泌细胞因子的影响。亚油酸和花生四烯酸抑制IL-4的分泌。这些实验系统将有助于筛选影响食品免疫反应的因素。此外，我们还测试了乳制品中使用的非致病性细菌干酪乳杆菌（Lactobacillus casei）对抗原诱导的IgE分泌的影响。干酪乳杆菌通过诱导巨噬细胞分泌IL-12抑制IgE的产生。结果表明，这种生物在预防ige介导的过敏中有潜在的用途。

We tested the effects of various environmental chemicals, beta-benzene hexachloride (beta-BHC), p, p'-dichlorodiphenyldichloroethylene (DDE), p-nitrophenyl 2, 4, 6-trichiorophenyl ether (CNP), D-alpha-aminobenzylpenicillin (Amp), o-acetylcitricacidtributyl ester (ATBC) on the immune response. All of the chemicals affectedcytokine secretion by lymph node cells in response to antigen. The chemicals also affected the serum antibody response elicited by oral administration of antigen. Feeding ovalbumin (OVA) to OVA-specific T cell receptor transgenic mice inducedinterleukin (IL)-4-secreting cells in the spleen and a serum IgE antibody response. Next this system was used to examine the effect of chemicals on immune responses related to allergic reactions. It was found that ATBC inhibited IL-4-production in this system. Further, substances involved in lipid metabolism were tested for their effect on cytokine secretion of T cells from these T cell receptor transgenic mice. Linoleic acid and arachidonic acid inhibited IL-4 secretion. These experimental systems should be useful for screening of factors affecting immune responses in food stuff.In addition, the effect of Lactobacillus casei, a nonpathogenic bacterium used in dairy products on antigen-induced IgE secretion was tested L.casei inhibited IgE production through induction of IL-12 secretion by macrophages. The results suggest a potential use of this organism in preventing IgE-mediated allergy.