Screening of factors triggering food allergy and research on development of hypoallergic food products.

食物过敏诱发因素筛选及低过敏食品开发研究。

• 批准号: 08556020
• 负责人: KAMINOGAWA Shuichi
• 金额: $ 6.08万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08556020/
• 关键词: food allergy; chemicals; IgE; Lactobacillus; T cell; interleukin (IL) -4; interleukin (IL) -12; 抗体応答; T細胞レセプタートランスジェニックマウス; インターロイキン4; インターロイキン5; オバルブミン; 環境物質; 細胞増殖; サイトカイン

We tested the effects of various environmental chemicals, beta-benzene hexachloride (beta-BHC), p, p'-dichlorodiphenyldichloroethylene (DDE), p-nitrophenyl 2, 4, 6-trichiorophenyl ether (CNP), D-alpha-aminobenzylpenicillin (Amp), o-acetylcitricacidtributyl ester (ATBC) on the immune response. All of the chemicals affectedcytokine secretion by lymph node cells in response to antigen. The chemicals also affected the serum antibody response elicited by oral administration of antigen. Feeding ovalbumin (OVA) to OVA-specific T cell receptor transgenic mice inducedinterleukin (IL)-4-secreting cells in the spleen and a serum IgE antibody response. Next this system was used to examine the effect of chemicals on immune responses related to allergic reactions. It was found that ATBC inhibited IL-4-production in this system. Further, substances involved in lipid metabolism were tested for their effect on cytokine secretion of T cells from these T cell receptor transgenic mice. Linoleic acid and arachidonic acid inhibited IL-4 secretion. These experimental systems should be useful for screening of factors affecting immune responses in food stuff.In addition, the effect of Lactobacillus casei, a nonpathogenic bacterium used in dairy products on antigen-induced IgE secretion was tested L.casei inhibited IgE production through induction of IL-12 secretion by macrophages. The results suggest a potential use of this organism in preventing IgE-mediated allergy.

我们测试了不同的环境化学物质--六六六(β-BHC)、对二氯二苯基二氯乙烯(DDE)、对硝基苯基2，4，6-三氯苯醚(CNP)、D-氨基青霉素(AMP)、邻乙酰柠檬酸三丁酯(ATBC)对免疫应答的影响。所有这些化学物质都会影响淋巴结细胞对抗原的反应分泌细胞因子。这些化学物质还会影响口服抗原引起的血清抗体反应。将卵清蛋白(OVA)喂给OVA特异性T细胞受体转基因小鼠，可诱导脾中分泌白介素4的细胞和血清中的IgE抗体反应。接下来，该系统被用来检测化学物质对与过敏反应相关的免疫反应的影响。结果发现，ATBC抑制该系统IL-4的产生。此外，还测试了与脂质代谢有关的物质对这些T细胞受体转基因小鼠T细胞分泌细胞因子的影响。亚油酸和花生四烯酸抑制IL-4的分泌。此外，还测试了乳制品中非致病细菌干酪乳杆菌对抗原诱导的IgE分泌的影响。干酪乳杆菌通过诱导巨噬细胞分泌IL-12来抑制IgE的产生。这些结果表明，这种微生物在预防IgE介导的变态反应方面具有潜在的用途。

项目成果

T.Yoshida ら: "The oral administration of low-dose antigen induces activation followed by tolerization,while high-dose antigen induces tolerance without activation." Clin.Immunol.Immunopathol.82. 207-215 (1997)

T. Yoshida 等人：“口服低剂量抗原会诱导激活，然后产生耐受，而高剂量抗原会诱导耐受而不激活。”Clin.Immunol.Immunopathol.82 (1997)。

M.Totsuka ら: "Antigen-specific inhibition of immune responses by antigen analogs with a single amino acid substitution." Animal Cell Technology : Basic & Applied Aspects. 8. 505-509 (1997)

M. Totsuka 等人：“具有单一氨基酸取代的抗原类似物对免疫反应的抗原特异性抑制。”动物细胞技术：基础与应用方面（1997）。

Y.Takahashi ら: "MHC class II/T cell receptor interactions potentiate secretion of IgG but not IgM in response to T-dependent antigens." Immunol.Lett.61. 97-101 (1998)

Y. Takahashi 等人：“MHC II 类/T 细胞受体相互作用增强了对 T 依赖性抗原的 IgG 分泌，但不增强 IgM 分泌。”Immunol.Lett.61 (1998)。

M.Kohyama ら: "Selective induction of CD8^+ T cell functions by single substituted analogs of an antigenic peptide : distinct signals for IL-10 production." FEBS Lett.423. 138-142 (1998)

M.Kohyama 等人：“通过抗原肽的单一取代类似物选择性诱导 CD8^+ T 细胞功能：IL-10 产生的独特信号。” FEBS Lett.423 (1998)。

Y.Takahashi ら: "Autocrine B cell stimulation by interleukin-2 during a cognate interaction with T cells." Immunol.Letters. 65. 183-188 (1999)

Y. Takahashi 等人：“白细胞介素 2 在与 T 细胞相互作用过程中刺激自分泌 B 细胞。”《免疫学快报》。