Purification, identification and functional analysis of immune suppressive factol

免疫抑制因子的纯化、鉴定及功能分析

• 批准号: 02454065
• 负责人: KAMINOGAWA Shuichi
• 金额: $ 4.03万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454065/
• 关键词: immune suppressive factor; IL-10; suppressor T cell; immune tolerance; food allergen; 抑制因子

CD8+ suppressor T cell clone 13G2 was established from lymph node of bovine si-casein immunized C57BL/6 mice. The clone suppressed proliferation of Thl clones induced by antigen plus antigen-presenting cells (APC) without any detectable cytolytic activity for responding cells. The suppression was not antigen specific and was not restricted to MHC. However, the clonec did not inhibit IL-2 induced Thl-proliferatiol) (1). The supernatant of 13G2 cultured with rIL-2 also suppressed antigen-induced proliferation of Thl clones, but did not that of Th2 clones. Target cells for the suppressor function was APC. Anti-IL-lO antibody SXC-I. blocked this suppressive activity. 13G2 clone produced IL-10 as revealed by Western blot analysis. This indicates that the suppressive lymphokine produced by 13G2 clone is IL-10 (2). A PCR analysis has shown that CD8+ cells of immunized lymph node express IL-10 mRNA upon antigenic stimulation. It is. possible that IL-10 play an important role in the immune suppression mediated by CD8+ lymphocytes.(1) T. Hisatsune et al. (1990) J. lmmunol., 145 : 2421(2) T. Hisatsune et al. (1992) Lymphokine & Cytokine Res., in press

从牛单酪蛋白免疫的C57BL/6小鼠的淋巴结中建立了CD8+抑制T细胞克隆13G2。该克隆抑制了由抗原加抗原提呈细胞(APC)诱导的Th1克隆的增殖，对反应细胞没有检测到任何细胞杀伤活性。这种抑制不是抗原特异性的，也不限于MHC。但克隆不能抑制IL-2诱导的Th1细胞增殖(1)。与rIL-2共同培养的13G2细胞上清液也能抑制抗原诱导的Th1克隆的增殖，但不能抑制Th2克隆的增殖。抑制因子功能的靶细胞是APC。抗IL-LO抗体SXC-I。阻止了这种抑制活动。Western印迹分析显示，13G2克隆产生IL-10。提示13G2克隆产生的抑制性淋巴因子为IL-10(2)。免疫后的淋巴结CD8+细胞在抗原刺激下表达IL-10mRNA。它是。IL-10可能在CD8+淋巴细胞介导的免疫抑制中起重要作用。(1)T.Hisatsune等。(1990)J.lmmunol.145：2421(2)T.Hisatsune et al.(1992)淋巴因子和细胞因子研究

项目成果

Hisatsune T.: "A suppressive lymphokine derived from Ts clone 13G2 is ILー10" Lymphokine and Cytokine Research. 11. 87-93 (1992)

Hisatsune T.：“源自 Ts 克隆 13G2 的抑制性淋巴因子是 IL-10”，淋巴因子和细胞因子研究，11. 87-93 (1992)。

Hisatsune T.: "A suppressive lymphokine derived from Ts clone 13G2 is IL-10" Lymphokine and Cytokine Research. 11. 87-93 (1992)

Hisatsune T.：“源自 Ts 克隆 13G2 的抑制性淋巴因子是 IL-10”淋巴因子和细胞因子研究。