Purification, identification and functional analysis of immune suppressive factol

在牛si-酪蛋白免疫C57BL/6小鼠淋巴结建立CD8+抑制T细胞克隆13G2。该克隆抑制了由抗原+抗原呈递细胞（APC）诱导的Thl克隆的增殖，但对应答细胞没有检测到的细胞溶解活性。这种抑制不是抗原特异性的，也不局限于MHC。然而，克隆不抑制IL-2诱导的thl -增殖(1)。il -2培养的13G2上清液对Thl克隆的增殖也有抑制作用，但对Th2克隆没有抑制作用。抑制功能的靶细胞为APC。抗il - lo抗体sgc - i。阻断这种抑制活性。Western blot结果显示，13G2克隆产生IL-10。这表明13G2克隆产生的抑制淋巴因子是IL-10(2)。PCR分析表明免疫淋巴结的CD8+细胞在抗原刺激下表达IL-10 mRNA。它是。IL-10可能在CD8+淋巴细胞介导的免疫抑制中发挥重要作用。(1) T. Hisatsune et al. (1990) J.免疫学。(2)张志刚等。（1992）淋巴因子与细胞因子研究，出版

CD8+ suppressor T cell clone 13G2 was established from lymph node of bovine si-casein immunized C57BL/6 mice. The clone suppressed proliferation of Thl clones induced by antigen plus antigen-presenting cells (APC) without any detectable cytolytic activity for responding cells. The suppression was not antigen specific and was not restricted to MHC. However, the clonec did not inhibit IL-2 induced Thl-proliferatiol) (1). The supernatant of 13G2 cultured with rIL-2 also suppressed antigen-induced proliferation of Thl clones, but did not that of Th2 clones. Target cells for the suppressor function was APC. Anti-IL-lO antibody SXC-I. blocked this suppressive activity. 13G2 clone produced IL-10 as revealed by Western blot analysis. This indicates that the suppressive lymphokine produced by 13G2 clone is IL-10 (2). A PCR analysis has shown that CD8+ cells of immunized lymph node express IL-10 mRNA upon antigenic stimulation. It is. possible that IL-10 play an important role in the immune suppression mediated by CD8+ lymphocytes.(1) T. Hisatsune et al. (1990) J. lmmunol., 145 : 2421(2) T. Hisatsune et al. (1992) Lymphokine & Cytokine Res., in press