Development of Rapid Analysis and Monitoring system for Microcystin

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The fast analysis of microcystin in environmental water was examined by using a protein phosphatase 2A inhibition assay. The heat treatment method was developed for rapid extraction of microcystin from Microcystis cells.Quantitative analysis of microcystin by the protein phosphatase 2A inhibition assay required two hours. The assay was accurate and reproducible. The cultured Microcystis suspension was submitted to the protein phosphatase 2A inhibition assay as well as commercial ELISA assay kit with similar results. But sensitivity of this assay was not so good as the ELISA assay kit. The microcystins were extracted from Microcystis cells by heat conditions such as temperature of 50-60℃ and heating time of more than 20 minutes. The heat treatment with-amylase, cellulase and lysozyme was slightly facilitated to extraction of it.Uses of the protein phosphatase 2A inhibition assay with the heat treatment method facilitate investigation of microcystin in natural water.The Monitoring system for Microcystin was made in three parts, such as extraction, concentration and detection equipment.The microcystins were extracted from Microcystis cells by heat treatment in extraction part. Extracted microcystins were concentrated by ultra-filtration in concentration part. And, those were determined by inhibition assay use of immobilization of protein phosphatase in detection part.In this study, any type microcystins was not detected in samples of resource water for drinking and samples of tap water. And, high concentration of microcystins was detected in samples of pond for agriculture. Concentration of microcystins was not related with Microcystis cell Numbers and/or water temperature.