Development of a cooling device for cryogenic X-ray and neutron Crystallography

低温X射线和中子晶体学冷却装置的研制

• 批准号: 11558086
• 负责人: NAKASAKO Masayoshi
• 金额: $ 6.27万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11558086/
• 关键词: Proteins; Radiation damage; Cryogenic Crystallography; Third-generation synchrotron facility; cryogenic cooling; Cryogenic protein crystallography; X-ray crystallography; 構造生物学; X線解析; X線回折; 放射光X線; 中性子回折

An open-flow cryostat using helium gas has been developed for cryogenic X-ray crystallography. The device is composed of a cryostat and a heat exchange apparatus, and dry nitrogen gas is supplied for shielding the cooled helium gas from atmosphere. The lowest temperature reached by the device is 35 K within a fluctuation of ±0.5 K at a flow rate of 6 litter/min. This device is easy to operate and enables to study crystal structures and the temperature-dependent phenomena occurring in biological macromolecules and materials above 35 K. In addition, The techniques in cryogenic experiments around the liquid nitrogen temperature have been applied to the structural determinations of various enzymes, the hydration structure analysis of proteins and the investigation of internal vibrational properties of proteins around the glass-transition temperature.

本文介绍了一种用于低温X射线晶体学的氦气开式恒温器。该装置由低温恒温器和热交换装置组成，并提供干燥的氮气用于将冷却的氦气与大气隔离。该装置的最低温度可达35 K，流速为6升/分，温度波动范围为±0.5 K。该装置操作简单，可用于研究生物大分子和材料在35 K以上的晶体结构和温度依赖现象。此外，液氮温度附近的低温实验技术已被应用于各种酶的结构测定、蛋白质的水合结构分析以及玻璃化转变温度附近蛋白质内部振动性质的研究。

项目成果

M. Nakasako, H. Nomura, H. Ogawa and C. Toyoshima: "Crystal structure analyses of membrane proteins: a case study for calcium pump from sarcoplasmic reticulum"The Japanese Journal of Pharmacology. 85 Suppl. l.. S1-1 (2001)

M. Nakasako、H. Nomura、H. Okawa 和 C. Toyoshima：“膜蛋白的晶体结构分析：肌浆网钙泵的案例研究”《日本药理学杂志》。

中迫雅由, 肥後順一: "水とタンパク質が織りなす生命活動の素過程"現代化学. (印刷中). (2002)

Masayoshi Nakasako、Junichi Higo：“与水和蛋白质交织的生命活动的基本过程”现代化学（出版中）。

M.Nakasako: "Large-scale networks of hydration water molecules around proteins investigated by cryogenic X-rayc crystallography"Cellular and Molecular Biology. 47. 767-790 (2001)

M.Nakasako：“通过低温 X 射线晶体学研究蛋白质周围的大规模水合水分子网络”细胞和分子生物学。

豊島近, 中迫雅由, 野村博美, 小川治夫: "筋小胞体カルシウムポンプの構造決定"放射光. 14. 204-210 (2002)

Chika Toyoshima、Masayoshi Nakasako、Hiromi Nomura、Haruo Okawa：“肌浆网钙泵的结构测定”同步辐射。14. 204-210 (2002)

I,Endo et al.: "Fe-type nitrile hyodratase"Journal of Inorganic Biochemistry. (in press). (2001)

I，Endo等人：“Fe型腈水合酶”无机生物化学杂志。