Study on the hydration structural changes in coupling with the internal motions of proteins using time-resolved fluorscence measurement

蛋白质在细胞中参与生命的基本过程，只有在水环境中才能折叠和发挥作用。为了理解为什么水对蛋白质是不可缺少的，有必要在原子水平的空间分辨率和时间分辨率上可视化蛋白质的水合结构。本课题通过开发蛋白质专用的时间分辨荧光测量系统，通过荧光测量和结构分析以及分子动力学模拟，从实验角度分析蛋白质水合动力学。

Proteins are engaged in the elementary processes of life in cells and fold and function only in aqueous environment. To understand why water is indispensable for proteins it is necessary to visualize the hydration structures of proteins at spatial resolution of atomic level and ps temporal resolution. In this research project, through developing time-resolved fluorescence measurement system dedicated for proteins, we analyzed the dynamics of protein hydration from the experimental point of view using the fluorescence measurement and structure analyses as well as molecular dynamics simulation.