Study on the hydration structural changes in coupling with the internal motions of proteins using time-resolved fluorscence measurement

蛋白质参与细胞中生命的基本过程，只有在水环境中才能折叠和发挥功能。为了理解为什么水对蛋白质来说是必不可少的，有必要在原子水平的空间分辨率和PS的时间分辨率上可视化蛋白质的水化结构。在本研究项目中，我们通过开发蛋白质时间分辨荧光测量系统，利用荧光测量、结构分析以及分子动力学模拟，从实验的角度分析了蛋白质水化动力学。

Proteins are engaged in the elementary processes of life in cells and fold and function only in aqueous environment. To understand why water is indispensable for proteins it is necessary to visualize the hydration structures of proteins at spatial resolution of atomic level and ps temporal resolution. In this research project, through developing time-resolved fluorescence measurement system dedicated for proteins, we analyzed the dynamics of protein hydration from the experimental point of view using the fluorescence measurement and structure analyses as well as molecular dynamics simulation.