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A Newly-Developed Method of Measuring Local Pulse Wave Velocity for Assessing of Severiy of Arteriosclerosis and Characteristics of Endotherial-Dependent Vasodilation

新开发的测量局部脉搏波速度的方法，用于评估动脉硬化的严重程度和内皮依赖性血管舒张的特征

基本信息

批准号：
11558110
负责人：
SENDA Shoichi
金额：
$ 8.58万
依托单位：
Kagawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

We propose a new method for local -measurement of the propagation velocity of a pulse wave for the quantitative evaluation of severity of atherosclerosis. This method uses a complex cross correlation of Doppler signals and, theoretically, has the potential for precise estimation of the propagation time of a pulse wave of less than 1 millisecond. Doppler signals shifted by blood flow are detected at two adjacent locations on the artery using the simultaneous two-gate pulsed Doppler method, stored in a computer, and then analyzed by cross correlation. Time delay at maximum correlation output gives propagation time of the pulse wave. The pulse wave velocity is calculated as the ratio between the distance separating the two transducers and the time delay. In an experimental study using a pulsatile. flow circuit model, regardless of sampling distance and flow velocity profile, there was little fluctuation in calculated pulse wave velocity. Practical velocity resolution. of this method was e … More stimated to be less than 2 m/sec, which is sufficient accuracy for measurement of local pulse wave velocity (LPWV) over a 2 cm distance. In a study in vivo of healthy volunteer, LPWV was faster in the femoral artery (6.4±0.8 m/sec) than in the carotid artery (4.8±0.5 m/sec) and abdominal aorta (4.0±0.5 m/sec).A new ultrasound system measuring LPWV has been developed, which can detect LPWV at 2 close points. The system is used to assess :1.LPWV foot-to-foot along abdominal aorta of normal and hypercholesterolemic rabbits. 2.Vascular responses to acetylcholine in conditions of inhibition of NO synthesis by L-NAME and augmentation of NO synthesis by L-NAME and L-arginine Results : (1)LPWV increases from proximal to distal along abdominal aorta of normal and hypercholesterolemic rabbits. In corresponding region, LPWV in hypercholesterolemic rabbits is higher than in normal ones, despite different sample distances. (2)L-NAME inhibits vascular relaxation to acetylcholine (Ach), Concurrent administration of L-arginine and L-NAME restores relaxation to Ach in normal rabbits, L-NAME attenuates relaxation to acetylcholine (Ach), L-arginine along with L-NAME slightly reverses relaxation to acetylcholine in hypercholesterolemic rabbits. (3)Plasma cyclic GMP is unchanged in situations of L-NAME alone or L-arginine plus L-NAME in normal and hypercholesterolemic rabbits. Conclusion : The new system is non-invasive and easy to be operated and can detect LPWV alterations, so it is worth being used to assess local vascular alteration Less

我们提出了一种局部测量脉搏波传播速度的新方法，用于定量评估动脉粥样硬化的严重程度。该方法使用多普勒信号的复杂互相关，理论上可以精确估计小于 1 毫秒的脉冲波的传播时间。使用同步双门脉冲多普勒方法在动脉上的两个相邻位置检测到因血流而移动的多普勒信号，并将其存储在计算机中，然后通过互相关进行分析。最大相关输出处的时间延迟给出了脉冲波的传播时间。脉搏波速度计算为两个换能器之间的距离与时间延迟之间的比率。在一项使用脉动的实验研究中。流路模型中，无论采样距离和流速分布如何，计算出的脉搏波速度波动很小。实用的速度分辨率。该方法的速度估计小于 2 m/sec，足以测量 2 cm 距离内的局部脉搏波速度 (LPWV)。在健康志愿者的体内研究中，股动脉（6.4±0.8 m/sec）的 LPWV 比颈动脉（4.8±0.5 m/sec）和腹主动脉（4.0±0.5 m/sec）更快。已经开发出一种测量 LPWV 的新超声系统，可以在 2 个近距离点检测 LPWV。该系统用于评估：1.正常兔和高胆固醇血症兔沿腹主动脉的足对足LPWV。 2.L-NAME抑制NO合成以及L-NAME和L-精氨酸增强NO合成条件下血管对乙酰胆碱的反应结果：(1)正常兔和高胆固醇血症兔的腹主动脉LPWV从近端到远端增加。在相应区域，尽管样本距离不同，高胆固醇血症兔的 LPWV 仍高于正常兔。 (2)L-NAME抑制血管对乙酰胆碱(Ach)的舒张，L-精氨酸和L-NAME同时给药可恢复正常兔对Ach的舒张，L-NAME减弱对乙酰胆碱(Ach)的舒张，L-精氨酸与L-NAME一起轻微逆转对高胆固醇血症兔的乙酰胆碱的舒张。 (3)正常兔和高胆固醇血症兔单独使用L-NAME或L-精氨酸加L-NAME时血浆环鸟苷酸没有变化。结论：新系统无创、操作方便，可检测LPWV改变，值得用于评估局部血管改变。