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Basic Studies on Sonodynamic Approaches of Cells by Ultrasound Activation.

超声激活细胞声动力学方法的基础研究。

基本信息

批准号：
14380408
负责人：
SENDA Shoichi
金额：
$ 9.47万
依托单位：
KAGAWA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14380408/
关键词：
ultrasound irradiation cell function cell activation microbubble

项目摘要

The purpose of this research project is to elucidate the matter whether a cell lesion done how and activation happens with the mechanical or chemical action, the heating action of the ultrasonic energy, and whether what happens together in the molecule level.The experimental device which can evaluate an influence on the cell of the ultrasonic energy quantitatively is indispensable by the research to aim at the treatment application. For this, ultrasonic waves, as for taking the frequency of the broad-band, as for pulse repetition rate being variability, as for ultrasonic output's being variability in the large area, as for what a focus can do it in a depth by, and, in such cases as SPTP or SATA, a condition. A power supply and a wattmeter and a high precision vibrator were combined, and a ultrasonic system was built, and a hydro-phone was included so that it could measure acoustic indices in the region of interest.This system was applied to the mosquito larva eradication research which … More was an urgent mission with WHO, and frequency, the pulse width, pulse repetition rate, and so on were changed to various setups of a condition, and the threshold level that a cytoclasis happened was investigated. Recently, the knowledge related to the research on the cell attack, destruction by the great physical strength ultrasonic waves and the clinical application was accumulated on the basic research to aim at treatment for a cancer by the genetic introduction based on the method of sonoporation, HIFU, and so on. However, or the research which revived a lesion cell didn't see big development in- or out-side the country. The preparation of the experiment device attained an expected purpose by this research, and a research about the critical condition of the cytoclasis was accumulated by this. However, a study about the condition of the cell activation wasn't satisfactory, and didn't reach ultrasonic activate effect actual proof that cell information transmission function activation was measured. It will go on more, and research will be developed. Less

本研究的目的是阐明细胞损伤是如何在机械作用或化学作用、超声能量的加热作用以及在分子水平上共同发生作用的情况下发生和激活的。能够定量评价超声能量对细胞影响的实验装置是针对治疗应用的研究必不可少的。对于这一点，超声波，如取宽带的频率，脉冲重复频率是可变性的，超声波输出的在大范围内是可变性的，关于焦点在深度上能做什么，在SPTP或SATA等情况下，是一种条件。将电源、瓦特计和高精度可控震源相结合，建立了超声波系统，并配备了水听器，以测量感兴趣区域的声学指标。该系统已应用于…更多的是与WHO的一项紧急任务，改变频率、脉冲宽度、脉冲重复率等来改变条件的不同设置，并调查发生细胞破裂的阈值水平。近年来，针对基于超声、HIFU等方法的基因导入治疗肿瘤的基础研究，积累了大体力超声波对细胞的攻击、破坏及其临床应用的相关研究知识。然而，重振病变细胞的研究在国内外都没有看到大的发展。通过本研究，实验装置的研制达到了预期的目的，为细胞破裂的临界条件研究积累了一定的经验。然而，关于细胞激活条件的研究并不尽如人意，也没有达到超声激活效果的实际证据，证明细胞的信息传递功能被激活了。它将继续进行更多，研究将得到发展。较少