MicroScale Thermophoresis device

微型热泳装置

• 批准号: 459677197
• 金额: --
• 依托单位: Albert-Ludwigs-Universität Freiburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2021
• 资助国家: 德国
• 起止时间: 2020-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/459677197?language=en
• 关键词: MicroScale; Thermophoresis; device

The characterization of protein-ligand interactions represents an important method in enzymology and drug discovery and development. Moreover, the quantification, that is the determination of binding affinities, is of special importance. The MicroScale Thermophoresis (MST) device is required for the characterization and quantification of protein-ligand and protein-protein interactions as outlined in the project proposals of the applicants. The binding experiments will provide new, important insights into the catalytic mechanisms of unique enzymes and will contribute to drug development based on inhibitors of selected, medically relevant target enzymes. For some of the projects, binding studies have to be performed under strictly anaerobic conditions due to the oxygen-sensitivity of the enzymes or ligands. For all projects, experiments have to be performed in small volumes, and high throughput is of great advantage. For some of the projects, the used method must be compatible with the use of detergents. In contrast to other methods and devices, the MST-method and MST-device is ideally suited for all project requirements. Represented by the applicants, the MST-device will be used within several research consortia funded by the DFG, such as the RTGs 1976 and 2202, the Priority Programme 1927, the cluster of excellence CIBSS and the Collaborative Research Centre 850.

蛋白质-配体相互作用的表征是酶学和药物发现和开发的重要方法。此外，定量，即确定结合亲和力，是特别重要的。如申请人的项目提案中所述，需要MicroScale Thermophoresis（MST）装置来表征和定量蛋白质-配体和蛋白质-蛋白质相互作用。结合实验将为独特酶的催化机制提供新的重要见解，并将有助于基于选定的医学相关靶酶抑制剂的药物开发。对于一些项目，由于酶或配体的氧敏感性，结合研究必须在严格的厌氧条件下进行。对于所有项目，实验必须在小体积中进行，高通量具有很大的优势。对于某些项目，使用的方法必须与洗涤剂的使用兼容。与其他方法和设备相比，MST方法和MST设备非常适合所有项目要求。由申请人代表，MST设备将在DFG资助的几个研究联盟中使用，例如RTG 1976和2202，优先计划1927，卓越的CIBSS集群和合作研究中心850。