Non-destructive measurement techniques of metabolite molecules in cells of crop plants

作物细胞代谢物分子无损测量技术

基本信息

批准号：
11695078
负责人：
NONAMI Hiroshi
金额：
$ 4.03万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11695078/
关键词：
Plant-water relations cell wall oligosaccharides laser desorption ionization MALDI TOF-MS MALDI matrix 細胞計測

项目摘要

This project was undertaken to analyze cell metabolite molecules of crop plants during acclimation process under environmental stress conditions. Cell metabolites were planned to be analyzed with matrix-assisted laser desorption/ionization time-of-flight mass spectrometers (MALDI TOF-MS). Minute volume of cell solution was used for molecular analysis, but the analysis was not easy with MALDI due to lack of proper matices for laser ionization. However, we progressed for the development of new matrices for MALDI analyses, and we report results related to plant physiology research under environmental stress conditions and MALDI research as follows;1. Environmental stress researchInhibition of cell elongation was observed under various environmental stress conditions. Cell turgor in the zone of elongation of intact plants was kept constant under stress conditions examined except high temperature stress. Solute accumulation took place in cells when stress conditions were applied to plants. Turgor loss was observed in cells of plants grown under extremely high temperature. Rates of cell elongation were correlated with sizes of the growth-induced water potential under stress conditions, indicating that the speed of cell elongation was regulated by how much water was absorbed by cells in the zone of elongation.Additionally, formation of somatic embryos was studied with relation with plant hormones.Furthermore, books related to water relations in plant physiology and cell measurement techniques were published.2. Mass spectrometry researchMatrix chemicals were developed for MALDI TOF-MS for macromolecules. To study relations between fragmentation in mass measurements and matrix chemicals for MALDI, artificial polymers were synthesized, and structural characteristics of analytes and interactions of analyte-matrix were studied. Furthermore, oligosaccharides extracted from seaweed cell walls were successfully analyzed with MALDI.

该项目是为了在环境压力条件下在适应过程中分析作物植物的细胞代谢物分子。计划通过基质辅助激光解吸/电离飞行时间质谱器（MALDI TOF-MS）分析细胞代谢物。细胞溶液的微量体积用于分子分析，但是由于缺乏适当的激光电离晶格，因此MALDI的分析并不容易。但是，我们进步了用于开发MALDI分析的新矩阵，并报告了在环境压力条件和MALDI研究中与植物生理研究有关的结果； 1。在各种环境应力条件下观察到了环境应力研究抑制细胞伸长的抑制作用。除高温应激外，在检查的压力条件下，在延伸植物伸长区域的细胞膨胀保持恒定。当将应力条件应用于植物时，溶质积累发生在细胞中。在极高温度下生长的植物的细胞中观察到了Turgor损失。细胞伸长的速率与在压力条件下的生长诱导的水潜能的大小相关，表明细胞伸长的速度受到细胞在伸长延伸区域的吸收吸收的水的速度。质谱研究matrix化学物质是针对大分子的MALDI TOF-MS开发的。为了研究质量测量中的碎片化与MALDI的基质化学物质之间的关系，研究了人工聚合物，并研究了分析物的结构特征和分析物 - 矩阵的相互作用。此外，用MALDI成功分析了从海藻细胞壁提取的寡糖。