Study of Space and Time Distribution of Precipitation in Tropics by Doppler Radars

多普勒雷达研究热带降水时空分布

• 批准号: 12440164
• 负责人: TERAZIMA Masahide
• 金额: $ 10.37万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12440164/
• 关键词: transient grating; volume change; enthalpy change; diffusion coefficient; photoreaction; protein; folding; 拡散; 無輻射遷移; 励起状態; 溶液

New photo-trigger methods were developed to study the protein dynamics and the energy changes during the chemical reactions of the proteins.1. Protein dynamic of carboxymyoglobin was studied using the photo-dissociation reaction of the ligand. The dynamics and the energy change were revealed from nanosecond to second time range.2. A pH jump method was developed to detect the protein structural change induced by the pH jump. The mechanism of the chemical reaction was studied.3. Dynamics of apo-myogobin induced by the pH jump reaction was revealed.4. Protein dynamics of cytochrome c was induced by photo-reduction method with a nanosecond pulsed laser. Thestructural change of cytochrome c after the photo-reduction was detected in terms of the volume change and the diffusion coefficient.5. The photo-isomerization reaction of Photoactive Yellow Protein was used to initiate the cyclic reaction. A large temperature dependent volume change and change of the diffusion coefficient were discovered for the first time. The role of amino acids was revealed using the mutation technique.6. A new reaction intermediate of octopus rhodopsin was discovered and the energy scheme of the reaction was determined.

发展了新的光触发方法来研究蛋白质的动力学和蛋白质在化学反应过程中的能量变化。利用配体的光解离反应研究了羧基肌球蛋白的蛋白质动力学。揭示了从纳秒到秒的动力学和能量变化。建立了一种检测pH跃变引起的蛋白质结构变化的pH跳跃方法。对化学反应机理进行了研究。揭示了pH跳跃反应诱导的脱脂肌红蛋白的动态变化。用纳秒脉冲激光光还原法诱导细胞色素c的蛋白质动力学。从体积变化和扩散系数两个方面检测光还原后细胞色素c的结构变化。利用光活性黄蛋白的光异构化反应引发环化反应。首次发现了随温度变化较大的体积变化和扩散系数的变化。利用突变技术揭示了氨基酸的作用。发现了章鱼视紫红质的一种新的反应中间体，并确定了该反应的能谱。

项目成果

A.Ukai: "Diffusion of organic molecules in the excited triplet states detected by the transient grating with a high wavenumber"J.Phys.Chem.A. 104. 6681-6688 (2000)

A.Ukai：“高波数瞬态光栅检测到激发三重态有机分子的扩散”J.Phys.Chem.A。

K.Takeshita, N.Hirota, Y.Ijftiamoto, M.Kataoka, F.Tokunaga;, M.Terazima: "Temperature dependent volume change of initial step of the photoreaction of Photoactive Yellow Protein studied by the transient grating"J.Am.Chem.Soc.. 122. 8524-8528 (2000)

K.Takeshita、N.Hirota、Y.Ijftiamoto、M.Kataoka、F.Tokunaga、M.Terazima：“通过瞬态光栅研究光活性黄色蛋白光反应初始步骤的温度依赖性体积变化”J.Am。

M.Sakakura: "Dynamics of structure and energy of horse carboxymyoglobin after photodissociation of the carbon monoxide"J.Am.Chem.Soc.. 123. 4286-4294 (2001)

M.Sakakura：“一氧化碳光解后马碳氧肌红蛋白的结构和能量的动力学”J.Am.Chem.Soc.. 123. 4286-4294 (2001)

A.Ukai, N Hirota, M.Terazima: "Radical diffusion measured by the transient grating in a short time scale"Chem. Phys. Lett.. 319. 319-427 (2000)

A.Ukai、N Hirota、M.Terazima：“短时间尺度内瞬态光栅测量的自由基扩散”Chem。

J.Choi: "A pH jump reaction studied by the transient grating method:Photodissociation of o-nitro-benzaldehyde"J.Phys.Chem.A. 105. 12-18 (2001)

J.Choi：“通过瞬态光栅法研究的pH跳跃反应：邻硝基苯甲醛的光解离”J.Phys.Chem.A。