Study of Space and Time Distribution of Precipitation in Tropics by Doppler Radars

发展了新的光触发方法来研究蛋白质动力学和蛋白质化学反应过程中的能量变化.利用配体的光解反应研究了碳氧肌红蛋白的蛋白质动力学。揭示了纳秒到秒量级的动力学过程和能量变化.建立了一种检测pH突变引起的蛋白质结构变化的方法。探讨了化学反应的机理.揭示了pH跃变反应诱导脱辅基肌红蛋白的动力学.利用纳秒脉冲激光光还原法诱导细胞色素c的蛋白质动力学。从体积变化和扩散系数两方面考察了光还原后细胞色素c的结构变化.利用光敏黄蛋白的光异构化反应引发环化反应。首次发现了大的温度依赖性体积变化和扩散系数的变化。利用突变技术揭示了氨基酸的作用.发现了一种新的章鱼视紫红质反应中间体，确定了反应的能量方案。

New photo-trigger methods were developed to study the protein dynamics and the energy changes during the chemical reactions of the proteins.1. Protein dynamic of carboxymyoglobin was studied using the photo-dissociation reaction of the ligand. The dynamics and the energy change were revealed from nanosecond to second time range.2. A pH jump method was developed to detect the protein structural change induced by the pH jump. The mechanism of the chemical reaction was studied.3. Dynamics of apo-myogobin induced by the pH jump reaction was revealed.4. Protein dynamics of cytochrome c was induced by photo-reduction method with a nanosecond pulsed laser. Thestructural change of cytochrome c after the photo-reduction was detected in terms of the volume change and the diffusion coefficient.5. The photo-isomerization reaction of Photoactive Yellow Protein was used to initiate the cyclic reaction. A large temperature dependent volume change and change of the diffusion coefficient were discovered for the first time. The role of amino acids was revealed using the mutation technique.6. A new reaction intermediate of octopus rhodopsin was discovered and the energy scheme of the reaction was determined.