Development of a new detection method of time resolved circular dichroism
时间分辨圆二色性新检测方法的开发
基本信息
- 批准号:08554021
- 负责人:
- 金额:$ 10.82万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (A)
- 财政年份:1996
- 资助国家:日本
- 起止时间:1996 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The circular dichroism (CD) is a very powerful technique to study molecular structures such as proteins. However, in spite of the potential capability, the applicability has been rather limited so far. One of the reason is that it is difficult to use the CD spectrometer for the time resolved study. We have developed two techniques to detect the time-resolved CD signal.1.A commercial CD spectrometer was improved to obtain a millisecond time resolution. The CD signal was detected directly from the spectrometer without thepolarization modulation.2.The conventional CD spectrometer uses a temporal modulation of the right-and left-polarized light intensity. We developed a new CD detection method using a spatial modulation of the polarized light intensity. The CD signal was detected by one of nonlinear optical spectroscopies, the transient grating technique. It was found that the sensitivity and the time resolution (10 ns) are excellent bacause of the back-ground free detection.We are now planning to apply this method to structural changes of some biological molecules.
圆二色性(CD)是研究蛋白质等分子结构的一种非常有效的技术。然而,尽管有潜在的能力,适用性一直相当有限,到目前为止。其中一个原因是CD谱仪难以进行时间分辨研究。我们发展了两种探测时间分辨CD信号的技术:1.改进了商用CD谱仪,使其具有毫秒级的时间分辨。CD信号直接从光谱仪中检测,而不需要偏振调制。2.传统的CD光谱仪使用左右偏振光强度的时间调制。我们发展了一种新的CD检测方法,使用偏振光强度的空间调制。CD信号的检测采用非线性光学光谱技术之一的瞬态光栅技术。实验结果表明,由于无背景探测,该方法具有很好的灵敏度和时间分辨率(10ns),我们正计划将其应用于某些生物分子的结构变化。
项目成果
期刊论文数量(35)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
M.Terazima: "Translational diffusion of chemically stable and reactive radicals in solution." Chem.Soc.Farad.Trans.92. 3057-3062 (1996)
M.Terazima:“化学稳定和反应性自由基在溶液中的平移扩散。”
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- 影响因子:0
- 作者:
- 通讯作者:
M.Terazima: "Advances in multiphoton processes and spectroscopy" World Scientific, 96 (1996)
M.Terazima:“多光子过程和光谱学的进展”世界科学,96 (1996)
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- 影响因子:0
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M.Terazima: "Refractive index change by photothermal effect with a constant density detected as temperature grating in various fluids." J.Chem.Phys.104. 4988-4998 (1996)
M.Terazima:“光热效应引起的折射率变化,以恒定密度检测为各种流体中的温度光栅。”
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- 发表时间:
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- 影响因子:0
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T.Hara: "A new application of the transient grating method to a photochemical reaction ; the entropy, reaction volume and partial molar volume measurements" J.Phys.Chem.100. 10194-10200 (1996)
T.Hara:“瞬态光栅方法在光化学反应中的新应用;熵、反应体积和偏摩尔体积测量”J.Phys.Chem.100。
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- 影响因子:0
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M.Takezaki: "Excited state dynamics of 9,10-diazaphenanthrene studied by the time-resolved transient grating method." J.Phys.Chem.100. 10015-10020 (1996)
M.Takezaki:“通过时间分辨瞬态光栅方法研究 9,10-二氮杂菲的激发态动力学。”
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TERAZIMA Masahide其他文献
TERAZIMA Masahide的其他文献
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{{ truncateString('TERAZIMA Masahide', 18)}}的其他基金
Creation of time-resolved thermodynamics and application to elucidation of protein reaction mechanism
时间分辨热力学的创建及其在阐明蛋白质反应机制中的应用
- 批准号:
18205002 - 财政年份:2006
- 资助金额:
$ 10.82万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Conformational changes and fluctuations of proteins in wide range during reactions
反应过程中蛋白质大范围的构象变化和波动
- 批准号:
13853002 - 财政年份:2001
- 资助金额:
$ 10.82万 - 项目类别:
Grant-in-Aid for Scientific Research (S)
Study of Space and Time Distribution of Precipitation in Tropics by Doppler Radars
多普勒雷达研究热带降水时空分布
- 批准号:
12440164 - 财政年份:2000
- 资助金额:
$ 10.82万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Elementary process from electronic energy to thermal energy and the quantum effect to molecular diffusion process
从电子能到热能的基本过程以及量子效应到分子扩散过程
- 批准号:
10206202 - 财政年份:1998
- 资助金额:
$ 10.82万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas (B)
Studies of structural dynamics of protein and the coherent motion by nonlinear optical spectroscopy
非线性光谱研究蛋白质的结构动力学和相干运动
- 批准号:
10440173 - 财政年份:1998
- 资助金额:
$ 10.82万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of a new spectroscopy based on several transient lens effects and its application to chemical reactions
基于几种瞬态透镜效应的新型光谱学的开发及其在化学反应中的应用
- 批准号:
06453023 - 财政年份:1994
- 资助金额:
$ 10.82万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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