Studies of structural dynamics of protein and the coherent motion by nonlinear optical spectroscopy

A nobel method to measure the enthalpy and volume changes after any photochemical reactions wasdeveloped using the time-resolved transient grating （TG）技术。some photochemical reactions and demonstrated that this技术nique is applicable to many irreversible反应：The protein dynamics and The enthalpy change of Myoglobin after The photodissociation反应carbon monoxide was investigated by this TG technique. The escaping rate from Theprotein was found to be 700ps . The protein contracts about 5cm - D13 - D1/mol just after The COdissociation and then expands about 15 cm D13 D1/mol by the escaping process from the protein.2.Itwas found the volume change after the photoreaction of PYP （photoactive yellow protein）depends on temperature.3.A new optical heterodyne detection of the TG method without an active反馈系统可以开发一个化学species ammany species选择一个proper phase between the excitation and the probe light fields.4. a ultrafastthermalization rate后the photoexcitation of a molecule in solution was measured by a new方法（acoustic peak delay method） based on the TG技术nique with a ps time resolution. It wasfound that thermal energy was transferred to some selective solvent molecules among the firstshell of the solvent molecules in solution. the rate depends on the solvent and mostly the hydrogenbonding between the solvent and solute molecules. Furthermore， a new molecular集成系统；molecular heater-molecular热系统was developed to study the energy transfer process of the thermal energy in solution. This systemcan detect the thermal energy with a high temporal and spatial resolution。

1.A nobel method to measure the enthalpy and volume changes after any photochemical reactions was developed using the time-resolved transient grating (TG) technique. This technique was applied to some photochemical reactions and demonstrated that this technique is applicable to many irreversible reactions. The protein dynamics and the enthalpy change of Myoglobin after the photodissociation reaction of carbon monoxide was investigated by this TG technique. The escaping rate from the protein was found to be 700 ps. The protein contracts about 5 cmィイD13ィエD1/mol just after the CO dissociation and then expands about 15 cmィイD13ィエD1/mol by the escaping process from the protein.2.It was found that the volume change after the photoreaction of PYP (photoactive yellow protein) depends on temperature.3.A new optical heterodyne detection of the TG method without an active feedback system was developed. It was demonstrated that a chemical species among many species can be selected by choosing a proper phase between the excitation and the probe light fields.4.A ultrafast thermalization rate after the photoexcitation of a molecule in solution was measured by a new method (acoustic peak delay method) based on the TG technique with a ps time resolution. It was found that the thermal energy was transferred to some selective solvent molecules among the first shell of the solvent molecules in solution. The rate depends on the solvent and mostly the hydrogen bonding between the solvent and solute molecules. Furthermore, a new molecular integrated system; molecular heater-molecular thermometer system, was developed to study the energy transfer process of the thermal energy in solution. This system can detect the thermal energy with a high temporal and spatial resolution.