Creation of health care device employing micro analysis chip of trace amount of blood

近年来，我国高龄化人口快速发展，医疗费用的增加给国家财政带来了很大的压力。我们认为“预防”疾病很重要，这样他们才能过上健康的生活。为了实现这一目标，我们研究了一种廉价的、可处理的“医疗芯片”，它使我们能够通过分析从微量血液中提取的血清和淋巴细胞来检查健康和疾病状况。目前，利用半导体微加工技术在石英板上刻出了精细的反转毛细图案。然后，将其模压在PET板上，形成通道。通过在毛细管内壁涂覆MPC聚合物获得实际的生物膜，使毛细管内壁具有生物相容性。采用直径为70μm的SUS制管，将芯片削尖后的无痛针头，通过下游的电渗泵将数十纳米的游离血液注入到通道中。将在芯片中离心分离得到的血清转移到由离子敏感场效应晶体管（ISFET）组成的集成生物传感器中，测量健康指标值。在研究中，我们计划测量pH、Na+、K、O2、co2、BUN、葡萄糖和肌酐8个指标，同时完成pH、Na+、K+和BUN的测量。利用石英片制备了在高电场条件下形成抽送区域的EOF泵，在10伏特条件下抽取高流速的血液。此外，为了充分利用全血，还研究了T细胞和B细胞与淋巴细胞的分离。由于T细胞的电荷是B细胞的1.5倍，因此可以通过电泳分离。首先，聚乙二醇聚合物胶束的包覆抑制了淋巴细胞的zeta电位和吸附。由于储液池和废物的体积较小，在阳极和阴极上分别发生酸和碱反应。这个问题通过在储存库和废物之间插入一个导电盐桥来解决，从而使我们能够测量T细胞和B细胞的电泳速度。根据这一结果，首先在凝胶中加入正偏压以粘附它们，随后在电极上加入负偏压，使它们迁移。因此，T细胞和B细胞在毛细管中的分离首先是通过细胞迁移成功的。少

Recently, our county suffers from a serious issue that increasing the medical treatment expenses push a big pressure to the national finance because of rapid development of highly aged person numbers. We considered that "prevention" for the disease was important so that they lived their healthy life. For the goal, we studied creation of the cheap and disposal "Healthcare chip" witch enabled us to check health and illness conditions at home by analysis of a serum and lymphocytes extracted from a trace amount of a blood. At present, a fine reversal capillary pattern was engraved in a quartz plate employing semiconductor microfabrication technologies. Then, it was molded in a PET plate, forming a channel. The inner wall of the capillary was bio-compatible by coating the MPC polymer acquiring the actual bio-membrane. A several ten nano litter blood was injected to the channel by an electroosmosis (EOF) pump arranged at the downstream through the painless needle whose chip was sharpened chi … More p of a SUS made 70μm diameter tube. The serum which was obtained by centrifugal separation in the chip was transferred to an integrated bio-sensors consisting of ISFET (ion sensitive field effect transistor) and values of the health markers was measured. At the research, we planed measurement of 8 markers of pH, Na+, K, O2, C02, BUN, glucose and creatinine, while measurement of pH, Na+, K+ and BUN was finished. The EOF pump whose pumping region was formed at high electric field one was fabricated using a quartz plate, extracting a blood with a high flow rate at ten volts. Furthermore, to utilizing the whole blood completely, separation of T and B cells from lymphocytes was also studied. Since electric charge of the T cells is 1.5 times larger than that of B cells, they can be separated by electrophoresis. At first, the zeta potential and adsorption of lymphocytes were suppressed by coating of the PEG polymer micelles. It was found that acid and alkaline proceeded at an anode and a cathode, respectively, because volume of a reservoir and a waste was small. This problem was solved by inserting a electrically conductive salt bridge between the reservoir and the waste, thereby allowing us to measure the electrophoresis velocity of the T and B cells. Based on this result, at first a positive bias was added to the gel to adhere them and subsequently negative bias was added to the electrode, migrating them. Consequently, separation of the T and B cells in a capillary was firstly succeeded by migrating the cells. Less