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Creation of health care device employing micro analysis chip of trace amount of blood

利用微量血液微量分析芯片研制保健装置

基本信息

批准号：
12450289
负责人：
HORIIKE Yasuhiro
金额：
$ 10.43万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12450289/
关键词：
Healthcare device Bio chip Painless Needle Health marker Electroosmosis flow pump ISFET Lymphocyte separation Salt Bridge マイクロキャピラリ生体適合化

项目摘要

Recently, our county suffers from a serious issue that increasing the medical treatment expenses push a big pressure to the national finance because of rapid development of highly aged person numbers. We considered that "prevention" for the disease was important so that they lived their healthy life. For the goal, we studied creation of the cheap and disposal "Healthcare chip" witch enabled us to check health and illness conditions at home by analysis of a serum and lymphocytes extracted from a trace amount of a blood. At present, a fine reversal capillary pattern was engraved in a quartz plate employing semiconductor microfabrication technologies. Then, it was molded in a PET plate, forming a channel. The inner wall of the capillary was bio-compatible by coating the MPC polymer acquiring the actual bio-membrane. A several ten nano litter blood was injected to the channel by an electroosmosis (EOF) pump arranged at the downstream through the painless needle whose chip was sharpened chi … More p of a SUS made 70μm diameter tube. The serum which was obtained by centrifugal separation in the chip was transferred to an integrated bio-sensors consisting of ISFET (ion sensitive field effect transistor) and values of the health markers was measured. At the research, we planed measurement of 8 markers of pH, Na+, K, O2, C02, BUN, glucose and creatinine, while measurement of pH, Na+, K+ and BUN was finished. The EOF pump whose pumping region was formed at high electric field one was fabricated using a quartz plate, extracting a blood with a high flow rate at ten volts. Furthermore, to utilizing the whole blood completely, separation of T and B cells from lymphocytes was also studied. Since electric charge of the T cells is 1.5 times larger than that of B cells, they can be separated by electrophoresis. At first, the zeta potential and adsorption of lymphocytes were suppressed by coating of the PEG polymer micelles. It was found that acid and alkaline proceeded at an anode and a cathode, respectively, because volume of a reservoir and a waste was small. This problem was solved by inserting a electrically conductive salt bridge between the reservoir and the waste, thereby allowing us to measure the electrophoresis velocity of the T and B cells. Based on this result, at first a positive bias was added to the gel to adhere them and subsequently negative bias was added to the electrode, migrating them. Consequently, separation of the T and B cells in a capillary was firstly succeeded by migrating the cells. Less

近年来，我国高龄人口的快速增长，给国家财政带来了很大的压力，医疗费用的增长成为一个突出的问题。我们认为“预防”疾病是重要的，这样他们才能过上健康的生活。为了实现这一目标，我们研究了创造廉价和处置“医疗芯片”女巫使我们能够通过分析从微量血液中提取的血清和淋巴细胞来检查家中的健康和疾病状况。目前，采用半导体微加工技术在石英板上雕刻精细的反转毛细管图案。然后，将其模制在PET板中，形成通道。通过涂覆MPC聚合物获得实际的生物膜，使毛细管的内壁具有生物相容性。通过设置在下游的电渗泵，将几十纳米的血液通过其芯片被削尖的无痛针注入通道。 ...更多信息直径为70μm的SUS管的p。将在芯片中通过离心分离获得的血清转移到由ISFET（离子敏感场效应晶体管）组成的集成生物传感器中，并测量健康标志物的值。在研究中，我们计划测量pH、Na+、K+、O2、CO2、BUN、葡萄糖和肌酐8项指标，同时完成pH、Na+、K+和BUN的测量。使用石英板制造泵送区域在高电场1下形成的隔膜泵，以10伏的高流速抽取血液。此外，为了充分利用全血，还研究了从淋巴细胞中分离T和B细胞。由于T细胞的电荷比B细胞的电荷大1.5倍，所以它们可以通过电泳分离。首先，通过PEG聚合物胶束的包覆抑制了淋巴细胞的zeta电位和吸附。发现酸和碱分别在阳极和阴极进行，因为储液器和废物的体积小。这个问题通过在储液器和废物之间插入导电盐桥来解决，从而允许我们测量T和B细胞的电泳速度。基于该结果，首先将正偏压添加到凝胶以粘附它们，随后将负偏压添加到电极，使它们迁移。因此，T和B细胞在毛细管中的分离首先通过迁移细胞而成功。少