Molecular basis for decline of leaf function during senescence: Analysis of major determinants causing decline of the amount of Rubisco

衰老过程中叶片功能下降的分子基础:Rubisco含量下降的主要决定因素分析

基本信息

  • 批准号:
    12460028
  • 负责人:
  • 金额:
    $ 9.02万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2001
  • 项目状态:
    已结题

项目摘要

1) Rubisco was actively synthesized during expansion, but the amount of Rubisco synthesized rapidly declined just before full expansion. It already declined to about one fifth of the maximal amount at the time of fall expansion, and further declined to about one-tenth during senescence. The changes in the levels of rbcL and rbcS mRNAs were almost coordinated with those changes in the amount of Rubisco synthesized. Thus, the amount of Rubisco synthesized was primarily determined by the levels of rbcL and rbcS mRNAs throughout the life span of the leaves. Degradation of Rubisco became far more active than synthesis of Rubisco during senescence. Since the synthesis of Rubisco during senescence scarcely contributed to its amount, the degradation of Rubisco is the major determinant for the amount of Rubisco in the senescent leaves.2) The large subunit of Rubisco (LSU) was directly fragmented at Gly-329 into the 37-kDa and 16-kDa fragments by reactive oxygen species generated in the illuminated lysates of chloroplasts or intact chloroplasts. The LSU could also be cleaved into several other fragments by increasing the incubation time or the Fe^<2+> concentrations. All the cleavage sites were at or very close to the active site, just around the metal binding site within a radius of 12Å, indicating that proximity and favorable orientation are probably the most important parameter determining the cleavage sites. GS2 was also degraded by reactive oxygen species with the same manner as the LSU.
1) Rubisco was actively synthesized during expansion, but the amount of Rubisco synthesized rapidly declined just before full expansion.在秋季扩张时已下降至最大量的五分之一左右,在衰老时进一步下降至十分之一左右。 The changes in the levels of rbcL and rbcS mRNAs were almost coordinated with those changes in the amount of Rubisco synthesized. Thus, the amount of Rubisco synthesized was primarily determined by the levels of rbcL and rbcS mRNAs throughout the life span of the leaves. Degradation of Rubisco became far more active than synthesis of Rubisco during senescence.由于衰老过程中 Rubisco 的合成对其数量几乎没有贡献,因此 Rubisco 的降解是衰老叶片中 Rubisco 数量的主要决定因素。 2) Rubisco 大亚基 (LSU) 在 Gly-329 处被光照裂解物中产生的活性氧直接裂解成 37 kDa 和 16 kDa 片段。 叶绿体或完整的叶绿体。 The LSU could also be cleaved into several other fragments by increasing the incubation time or the Fe^<2+> concentrations.所有裂解位点都位于或非常接近活性位点,就在半径 12Å 内的金属结合位点周围,表明接近度和有利的方向可能是决定裂解位点的最重要参数。 GS2 was also degraded by reactive oxygen species with the same manner as the LSU.

项目成果

期刊论文数量(70)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

MAE Tadahiko其他文献

MAE Tadahiko的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('MAE Tadahiko', 18)}}的其他基金

Molecular and physiological basis of Rubisco turnover and its relation to nitrogen nutrition
Rubisco转换的分子和生理基础及其与氮营养的关系
  • 批准号:
    15380046
  • 财政年份:
    2003
  • 资助金额:
    $ 9.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Physiological and biochemical studies on leaf development and senescence : Degradation of Rubisco mediated by reactive oxygen species
叶片发育和衰老的生理生化研究:活性氧介导的 Rubisco 降解
  • 批准号:
    09460036
  • 财政年份:
    1997
  • 资助金额:
    $ 9.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
APPLICATION OF TISSUE CULTURE TECHNIQUE TO THE DEVELOPMENT OF ACID-SOIL TOLERANT PLANT.
组织培养技术在耐酸性土壤植物培育中的应用。
  • 批准号:
    05556010
  • 财政年份:
    1993
  • 资助金额:
    $ 9.02万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
Molecular Basis for Nitrogen Remobilization with Leaf Senescence
叶片衰老氮再动员的分子基础
  • 批准号:
    03454061
  • 财政年份:
    1991
  • 资助金额:
    $ 9.02万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

相似国自然基金

利用酵母RNA三杂交和叶绿体转化系统分析植物psbA和rbcL基因表达的顺反式调控因子
  • 批准号:
    30400268
  • 批准年份:
    2004
  • 资助金额:
    22.0 万元
  • 项目类别:
    青年科学基金项目
红豆杉科及其相关类群叶绿体rbcL基因序列的分支分析
  • 批准号:
    39700117
  • 批准年份:
    1997
  • 资助金额:
    10.0 万元
  • 项目类别:
    青年科学基金项目
角苔目叶绿体rbcl基因序列的分支分析
  • 批准号:
    39500013
  • 批准年份:
    1995
  • 资助金额:
    8.0 万元
  • 项目类别:
    青年科学基金项目
从基因工程的水稻rbcL和rbc化学组装成水稻Rubisco
  • 批准号:
    29572077
  • 批准年份:
    1995
  • 资助金额:
    9.0 万元
  • 项目类别:
    面上项目

相似海外基金

International Research Fellowship Program: Phylogenetic Analysis of the Phaeophyceae (Brown Algae) Based on rbcL and LSU Sequences
国际研究奖学金计划:基于 rbcL 和 LSU 序列的褐藻纲(褐藻)系统发育分析
  • 批准号:
    0107297
  • 财政年份:
    2001
  • 资助金额:
    $ 9.02万
  • 项目类别:
    Fellowship Award
Post-transcriptional Control of rbcL Gene Expression in the C4 dicots Amaranth and Flaveria Bidentis
C4 双子叶植物苋菜和黄顶菊中 rbcL 基因表达的转录后控制
  • 批准号:
    0110411
  • 财政年份:
    2001
  • 资助金额:
    $ 9.02万
  • 项目类别:
    Continuing Grant
シダ植物のrbcL遺伝子の塩基配列情報を一次キーにした生物学的種の認識
以蕨类植物rbcL基因碱基序列信息为主键的生物物种识别
  • 批准号:
    00J03497
  • 财政年份:
    2000
  • 资助金额:
    $ 9.02万
  • 项目类别:
    Grant-in-Aid for JSPS Fellows
Molecular α-taxonomy of Japanese ferns using rbcL sequence data as the first key information
使用 rbcL 序列数据作为第一个关键信息的日本蕨类植物的分子 α 分类学
  • 批准号:
    11440246
  • 财政年份:
    1999
  • 资助金额:
    $ 9.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Angiosperm Phylogeny: Evidence From rbcL, 18S rDNA, and atpB
被子植物系统发育:来自 rbcL、18S rDNA 和 atpB 的证据
  • 批准号:
    9707868
  • 财政年份:
    1997
  • 资助金额:
    $ 9.02万
  • 项目类别:
    Standard Grant
Molecular phylogenetic analyzes of pteridophytes based on rbcL sequence comparison
基于rbcL序列比较的蕨类植物分子系统发育分析
  • 批准号:
    04404003
  • 财政年份:
    1992
  • 资助金额:
    $ 9.02万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (A)
Phylogeny of Saxifragaceae and Relationships in Rosales Using rbcL Sequence Analysis
使用 rbcL 序列分析研究虎耳草科的系统发育和蔷薇科植物的亲缘关系
  • 批准号:
    9007614
  • 财政年份:
    1991
  • 资助金额:
    $ 9.02万
  • 项目类别:
    Continuing Grant
Phylogenetic Relationships in Cheilanthoid Ferns (Adiantaceae) Based on Chloroplast DNA Restriction Site Variation and rbcL Sequence Data
基于叶绿体 DNA 限制位点变异和 rbcL 序列数据的 Cheilanthoid Ferns(铁线蕨科)系统发育关系
  • 批准号:
    8817981
  • 财政年份:
    1989
  • 资助金额:
    $ 9.02万
  • 项目类别:
    Standard Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了