APPLICATION OF TISSUE CULTURE TECHNIQUE TO THE DEVELOPMENT OF ACID-SOIL TOLERANT PLANT.

我们试图评估胡萝卜植株起源于愈伤组织的耐酸性土壤，这些愈伤组织被选作耐铝（选择）细胞系。D_1和D_2种子均为有效种子，分别进行了土培试验和水培试验。在典型的酸性火山岩土壤上，选择的D1和D2植株的根系生长量大于未选择的D1和D2植株。在铝胁迫溶液培养体系中也得到了类似的结果。在选定的植物，吸附铝到他们的根显着减少。这些结果表明，在植物细胞培养系统中进行铝胁迫选择是培育耐酸性土壤植物新类型的有效方法。

We attempted to assess acid-soil-tolerance of carrot plants originated from calli which were selected as aluminum-tolerant (selected) cell lines. Both D1 and D2 seeds were avilable, and they were used for soil experiment and solution experiment. On typical acidic volcanic soil, root growth of selected D1 and D2 plants was more than that of non-selected D1 and D2 plants. Similar results were also obtained in the Al-stressed solution culture system. In selected plants, adsorption of Al to their root was significantly reduced. From these results, aluminum-stress selection in plant cell culture system is said to be effective method for development of new type of plants tolerant to acidic soils.