APPLICATION OF TISSUE CULTURE TECHNIQUE TO THE DEVELOPMENT OF ACID-SOIL TOLERANT PLANT.

组织培养技术在耐酸性土壤植物培育中的应用。

• 批准号: 05556010
• 负责人: MAE Tadahiko
• 金额: $ 4.22万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05556010/
• 关键词: Aluminum stress; Acid soil; Tissue culture; Cultured cells; Carrot; ストレス選抜; アルミニウム; 植物組織培養; ストレス耐性; ニンジン培養細胞

We attempted to assess acid-soil-tolerance of carrot plants originated from calli which were selected as aluminum-tolerant (selected) cell lines. Both D1 and D2 seeds were avilable, and they were used for soil experiment and solution experiment. On typical acidic volcanic soil, root growth of selected D1 and D2 plants was more than that of non-selected D1 and D2 plants. Similar results were also obtained in the Al-stressed solution culture system. In selected plants, adsorption of Al to their root was significantly reduced. From these results, aluminum-stress selection in plant cell culture system is said to be effective method for development of new type of plants tolerant to acidic soils.

我们试图评估胡萝卜植株起源于愈伤组织的耐酸性土壤，这些愈伤组织被选作耐铝（选择）细胞系。D_1和D_2种子均为有效种子，分别进行了土培试验和水培试验。在典型的酸性火山岩土壤上，选择的D1和D2植株的根系生长量大于未选择的D1和D2植株。在铝胁迫溶液培养体系中也得到了类似的结果。在选定的植物，吸附铝到他们的根显着减少。这些结果表明，在植物细胞培养系统中进行铝胁迫选择是培育耐酸性土壤植物新类型的有效方法。

项目成果

Koyama H,Ojima K,Yamaya T and Sonoda Y: Plant-soil interactions at low pH (Ed.) Date RA, "Characteristics of carrot plants regenerated from two cell lines selected as either ionic-Al tolerant cells or Al-phosphate using cells". Kluwer Academic Publishers

Koyama H、Ojima K、Yamaya T 和 Sonoda Y：低 pH 条件下的植物-土壤相互作用（主编）日期 RA，“使用细胞选择离子铝耐受细胞或磷酸铝细胞的两种细胞系再生胡萝卜植物的特征

Yokota S and Ojima K: Plant-soil interactions at low pH (Ed.), Date RA, "Physiological response of root-tip of alfalfa to low pH and aluminium stres in water culture". Kluwer Academic Publishers (Netherlands), 329-311 (1995)

Yokota S 和 Ojima K：低 pH 条件下植物与土壤的相互作用（Ed.），Date RA，“水培中苜蓿根尖对低 pH 值和铝胁迫的生理反应”。

Yokota S and Ojima K: "Plant-Soil Interactions at Low pH" Kluwer Academic Publishers. (Netherlands), 3 (1995)

Yokota S 和 Ojima K：“低 pH 值下的植物-土壤相互作用”Kluwer 学术出版社。

Koyama,H.,Ojima,K.,Yamaya,T.,Sonoda,Y.: "Characteristics of carrot plants regenerated from two cell lines selected as either ionic-Al tolerant cells or Al-phosphate using cells." Plant-soil interactions at low pH.(Ed).Date,R.A.,Kluwer Academic Publishers,

Koyama，H.，Ojima，K.，Yamaya，T.，Sonoda，Y.：“从选择为离子铝耐受细胞或铝磷酸盐使用细胞的两种细胞系再生的胡萝卜植物的特征。”

Koyama H,Ojima K,Yamaya.T and Sonoda Y: "Plant-Soil Interactions at Low pH" Kluwer Academic publishers (Netherlands), 5 (1995)

Koyama H、Ojima K、Yamaya.T 和 Sonoda Y：“低 pH 值下的植物-土壤相互作用”Kluwer 学术出版社（荷兰），5 (1995)