权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Mitochondrial genomics of fishes : determination and analysis of complete nucleotide sequence of the mtDNA

鱼类线粒体基因组学：线粒体DNA完整核苷酸序列的测定和分析

基本信息

批准号：
12460083
负责人：
NISHIDA Mutsumi
金额：
$ 10.11万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

Genetic information encoded on the DNA provides important makers for estimating genealogy, population structure, phylogeny, and evolution of organisms. However, it has become apparent that obtaining enough genetic information from the nuclear genome is a rather difficult task because the nuclear genome is a very complex entity in which many genes are duplicated. In this context, preferable is the mitochondrial (mt) genome because of its simplicity. Mitochondrial genomes (and their genes) in "advanced" animals are homologous undoubtedly and are inherited without recombination, and thus they are attractive for phylogenetic and population genetic researches as an important information source. In this project, we determined and compared complete nucleotide sequences of the mt genomes from many fishes representing major lineages of fish, and then tried to estimate comprehensive phylogenetic framework of teleostean fishes on the basis of the resultant large DNA dataset.Thanks to further development of experimental procedures, equipments, and our research group, we have sequenced complete mt genomes from more than 200 species, which number exceeded ten times as many as what we first aimed to sequence. From the sequence data, various new types of gene arrangement deviated from that of the common vertebrate were found. Phylogenetic analysis of the sequence data also provided many new findings on the relationships among basal teleosts. One of important findings is that the Elopomorpha which includes eels is found to be much more basal in the teleostean phylogeny than assumed previously.

编码在DNA上的遗传信息为估计生物的系谱、种群结构、生殖和进化提供了重要的标记。然而，很明显，从核基因组中获得足够的遗传信息是一项相当困难的任务，因为核基因组是一个非常复杂的实体，其中许多基因是重复的。在这种情况下，优选的是线粒体（mt）基因组，因为其简单。“高级”动物的线粒体基因组（及其基因）无疑具有同源性，不经重组而遗传，是系统发育和群体遗传学研究的重要信息来源。在本项目中，我们测定并比较了代表鱼类主要谱系的多种鱼类的mt基因组的完整核苷酸序列，然后尝试在所得到的大DNA数据集的基础上估计硬骨鱼类的全面系统发育框架。由于实验程序、设备和我们的研究小组的进一步发展，我们已经测定了200多个物种的mt基因组的完整核苷酸序列，这个数目超过了我们最初打算测序的十倍。从序列数据中发现了许多新的基因排列类型，这些基因排列类型与普通脊椎动物的基因排列类型不同，系统发育分析也为研究底栖硬骨鱼类的亲缘关系提供了新的发现。其中一个重要的发现是，包括鳗鱼在内的Elopomorpha在硬骨鱼类的生殖发育中比以前认为的要基础得多。