Construction of fish mitochondrial genome database

为了组织对水生生态系统中生物多样性和生物资源的保护或管理措施，必须提供关于种群变异性、种群结构、卵、幼虫或其他各种材料的物种鉴定等详细的遗传信息。然而，到目前为止，只有少数设施，如在线数据库，可用于野生生物的遗传信息。这一项目侧重于线粒体基因组，这是已知的丰富遗传信息的来源，旨在建立一个鱼类基因组的在线数据库。该项目已经成功地产生了许多关于鱼类线粒体基因组的完整核苷酸序列的数据，并通过该项目提交给了国际DNA数据库系统。在这些数据和以前提交的数据的基础上，鱼类线粒体基因组数据库被构建为国际在线数据库(http://mitofish.ori.u-tokyo.ac.jp/)This数据库“MitoFish”允许用户了解数据)。如果用户拥有鱼类线粒体基因组的部分核苷酸序列数据，“MitoFish”还允许他们针对其中的鱼类完整的线粒体基因组数据进行核苷酸序列的同源性搜索。使用这个搜索选项，用户可以找到数据库中与自己手中的材料关系最密切的鱼类物种或群体(S)。这个数据库有许多与有用的国际数据库的链接，因此用户能够很容易地获得有关鱼类的参考、标本、分类和生物学的各种信息。随着国际DNA数据库的不断更新，“MitoFish”也将成长为越来越独特和有用的鱼类线粒体基因组数据库。

For organizing protection or management measures for biodiversity and biological resources in the aquatic ecosystems, indispensable is detailed genetic information on variability of populations, population structure, species identification of eggs, larvae, or other various kinds of materials, and so forth. However, only few facilities, such as online databases, have been available so far for genetic information on organisms in the wild.This project focused on the mitochondrial genome, which is known as a source of rich genetic information for such purposes, and aimed to construct an on-line database of the genome of fishes. Much data on the complete nucleotide sequence of the mitochondrial genome in fishes have been successfully generated and submitted to the international DNA database system by this project. On the basis of those and previously submitted data, the fish mitochondrial genome database was constructed as an international on-line database (http://mitofish.ori.u-tokyo.ac.jp/)This database "MitoFish" allows the users to know data.availability of complete nucleotide sequence of mitochondrial genome of fishes in the international DNA database system (DDBJ/GenBank/EMBL). If the users have partial nucleotide sequence data from fish mitochondrial genome, "MitoFish" also allows them to conduct homology search of the nucleotide sequence against the fish complete mitochondrial genome data in it. Using this search option, users can find what fish species or group(s) in the database is the most closely related to the material in their hand. This database has a lot of interlinks to useful international databases so that users are able to easily obtain various information on reference, specimen, taxonomy, and biology for the fish concerned. Through continuous update following the growth of the/international DNA databases, "MitoFish" will also grow into more and more unique and useful database of fish mitochondrial genome.