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Towards establishment of mitochondrial genomics of marine animals

建立海洋动物线粒体基因组学

基本信息

批准号：
15380131
负责人：
NISHIDA Mutsumi
金额：
$ 10.05万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380131/
关键词：
marine animals fishes crustaceans mitochondrial DNA mitochondrial genomics phylogenetic analysis DNA marker species identification mtDNA 分子系統種査定

项目摘要

Proper measures for sustainable use of aquatic bioresources are urgently needed. To take such measures to manage aquatic organisms is not an easy task, however, because it is usually difficult to understand their population structure and even to identify their eggs and larvae. Therefore, reliable DNA markers for population and species identification are needed to be much developed. This study focused on the mitochondrial DNA as a hopeful resource for such markers. In this study, we aimed to promote our analyses of complete mitochondrial genome of fishes and also to extend our target organisms to crustaceans and some other marine invertebrates in order to develop mitochondrial genomics of marine animals.We newly collected many specimens of fishes, crustaceans, and other marine invertebrates including those of rare but important species for comprehensive phylogenetic analyses. More than 200 species have been sequenced for their complete mitochondrial genomes, and much more number of species have been analyzed for their partial mitochondrial genomes. Those data were compared with the previous data from other species to examine their variability and characteristics. Based on those novel data from fishes, our fish mitochondrial genome databese, MitoFish, was updated. Using huge datasets of nucleotides obtained and powerful computing systems, we made extensive phylogenetic analyses, and proposed new phylogenetic hypotheses for various groups of fishes, crustaceans, and some other marine invertebrates with high reliability. On the basis of the phylogenetic trees newly obtained, we estimated divergence times of major lineages of teleostean fishes to reconstruct an evolutionary history of fishes.

迫切需要采取适当措施可持续利用水生生物资源。然而，采取这些措施来管理水生生物并不是一件容易的事，因为通常很难了解它们的种群结构，甚至很难识别它们的卵和幼虫。因此，需要开发可靠的种群和物种鉴定DNA标记。这项研究的重点是线粒体DNA作为这类标记的有希望的资源。在这项研究中，我们的目的是促进我们对鱼类线粒体全基因组的分析，并将我们的目标生物扩展到甲壳类和其他一些海洋无脊椎动物，以建立海洋动物的线粒体基因组学。我们最近收集了许多鱼类、甲壳类和其他海洋无脊椎动物的标本，包括一些罕见但重要的物种，用于全面的系统发育分析。超过200个物种的完整线粒体基因组已经被测序，更多的物种的部分线粒体基因组已经被分析。这些数据与之前其他物种的数据进行了比较，以检查它们的变异性和特征。基于这些来自鱼类的新数据，我们的鱼类线粒体基因组数据库MitoFish进行了更新。利用获得的庞大的核苷酸数据集和强大的计算系统，我们进行了广泛的系统发育分析，并对鱼类、甲壳类动物和其他一些海洋无脊椎动物的不同类群提出了新的系统发育假设，可靠性很高。在新获得的系统发育树的基础上，我们估计了硬骨鱼类主要谱系的分化时间，以重建鱼类的进化史。