MECHANISM OF INFECTION OF EPSTEIN-BARR VIRUS TO T AND NK CELLS

EB病毒感染T细胞和NK细胞的机制

• 批准号: 12470165
• 负责人: TSUCHIYA Shigeru
• 金额: $ 9.41万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470165/
• 关键词: EBV; REAL TIME PCR; RECOMBINANT EBV; T CELL; LEUKEMIA CELL LINE; CHEMOKINE RECEPTORS; CHRONIC EBV INFECTION; 血球貧食症候群; 血球貪食症候群

1) The method of real time PCR for EBV DNA of peripheral lymphocytes were established. Separation of lymphocyte subpopulations of the patients with EBV related diseases by the immunomagnetic bead method also enabled us to determine an EBV infected lymphocyte subset.2) L-MEG is the thymocyte derived cell line from T cell lymphoma, express CD21 EBV receptors and grows on the Balb/c feeder cells. As a model of EBV infection to T cells we tried to establish feeder cell independent cell lines of L-MEG by infection of recombinant EBV which contained neomycin resistant gene. We were able to confirm the expression of EBNA protein after infection with EBV, however, G418 resistant L-MEG cell lines were not yet established.3) We examined the expression of chemokine receptors on lymphocyte subsets of the patients with acute and chronic EBV infection. Upregulation and down regulation of the several chemokine receptors were observed. Since the number of the cases examined were still a few, further study was required.

1）建立了周围淋巴细胞EBV DNA实时PCR的方法。通过免疫磁珠方法对EBV相关疾病患者的淋巴细胞亚群的分离也使我们能够确定EBV感染的淋巴细胞亚群。2）L-MEG是T-MEG是T细胞淋巴瘤的胸腺细胞衍生的细胞系，Express CD21 EBV受体和在Balb/c Fielder上生长。作为对T细胞的EBV感染的模型，我们试图通过感染含有新霉素耐药基因的重组EBV来建立L-MEG的馈物细胞独立细胞系。我们能够确认用EBV感染后EBNA蛋白的表达，但是，尚未建立G418抗L-MEG细胞系。3）我们检查了急性和慢性EBV感染患者淋巴细胞群上趋化因子受体的表达。观察到了几种趋化因子受体的上调和下调。由于所检查的案件数量仍然有几个，因此需要进一步研究。

项目成果

Asao H et al.: "Cutting edge : the common gamma-chain is an indispensable subunit of the IL-21 receptor complex"J Immunol. 167. 1-5 (2001)

Asao H 等人：“前沿：共同的 γ 链是 IL-21 受体复合物不可或缺的亚基”J Nutrition。

Kumaki S., Villa A., Asada H., Kawai S., Ohashi Y., Takahashi M., Hakozaki I., Nitanai E., Minegishi M., Tsuchiya S.: "Identification of anti-herpes simplex virus antibody-producing B cells in a patient with an atypical RAG1 immunodeficiency"Blood.. 98. 1

Kumaki S.、Villa A.、Asada H.、Kawai S.、Ohashi Y.、Takahashi M.、Hakozaki I.、Nitanai E.、Minegishi M.、Tsuchiya S.：“抗单纯疱疹病毒抗体的鉴定-

Kumaki S et al.: "Identification of anti-herpes simplex virus antibody-producing B cells in a patient with an atypical RAG1 immunodeficiency"Blood. 98. 1464-1468 (2001)

Kumaki S 等人：“非典型 RAG1 免疫缺陷患者体内产生抗单纯疱疹病毒抗体的 B 细胞的鉴定”血液。

Sasahara Y., Fujie H., Kumaki S., Ohashi Y., Minegishi M., Tsuchiya S.: "Ebstein-Barr virus-associated hodakin's disease in a patient with Wiskott-Aldrich syndrome"Act a Paediatr.. 90. 1348-51 (2001)

Sasahara Y.、Fujie H.、Kumaki S.、Ohashi Y.、Minegishi M.、Tsuchiya S.：“Wiskott-Aldrich 综合征患者中与 Ebstein-Barr 病毒相关的霍达金病”儿科法案.. 90. 1348

Sasahara Y., Kumaki S., Ohashi Y., Minegishi M., Kano H., Bessho F., Tsuchiya S.: "Deficient activity of von Willebrand factor-cleaving protease in patients with Upshaw-Schulman syndrome"Int J Hematol.. 74. 109-14 (2001)

Sasahara Y.、Kumaki S.、Ohashi Y.、Minegishi M.、Kano H.、Bessho F.、Tsuchiya S.：“Upshaw-Schulman 综合征患者中冯维勒布兰德因子裂解蛋白酶的活性不足”Int J Hematol。