Leukemogenesis due to insertional mutagenesis-a NOG mouse model-

插入突变引起的白血病发生-NOG小鼠模型-

• 批准号: 19390280
• 负责人: TSUCHIYA Shigeru
• 金额: $ 11.56万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19390280/
• 关键词: NOGマウス; 遺伝子治療; レトロウイルスベクター; LMO2; MLL; AF10; 白血病; k-ras; セカンドヒット; レトロウィルスベクター; 挿入変異; 先天性免疫不全症; 動物モデル

Humanized NOD/shi-scid/gammac (null) (NOG) mice (hu-HSC NOG mice) model was established in order to know the safety and efficacy of human gene therapy. We found that LMO-2 gene-introduced human cord blood stem cells showed transient proliferation in NOG mice, but did not induce leukemia at all. The conclusion is that the second hit by an additional oncogene must be required to complete the leukemogenic process.

为了解人类基因治疗的安全性和有效性，建立了人源化NOD/shi-scid/gammac（null）（NOG）小鼠（hu-HSC NOG小鼠）模型。我们发现导入LMO-2基因的人脐带血干细胞在NOG小鼠中表现出短暂的增殖，但根本不会诱发白血病。结论是，白血病发生过程的完成必须需要额外的癌基因的第二次打击。

项目成果

Cell-cycle-dependent oscillation of GATA2 expression in hematopoietic cells

• DOI: 10.1182/blood-2006-08-044149
• 发表时间: 2007-05-15
• 期刊: BLOOD
• 影响因子: 20.3
• 作者: Koga, Shinichiro;Yamaguchi, Nobuhiro;Minegishi, Naoko
• 通讯作者: Minegishi, Naoko

Molecular and Clinical Analysis of RAF1 in Noonan Syndrome and Related Disorders: Dephosphorylation of Serine 259 as the Essential Mechanism for Mutant Activation

• DOI: 10.1002/humu.21187
• 发表时间: 2010-03-01
• 期刊: HUMAN MUTATION
• 影响因子: 3.9
• 作者: Kobayashi, Tomoko;Aoki, Yoko;Matsubara, Yoichi
• 通讯作者: Matsubara, Yoichi

初発時に両側腎に病変を認めたウィルムス腫瘍の4例

初发双肾病变的肾母细胞瘤4例

• 发表时间: 2009
• 作者: 風間理郎;土屋滋;他11名
• 通讯作者: 他11名

Connective tissue growth factor is overexpressed in muscles of human muscular dystrophy

• DOI: 10.1016/j.jns.2007.09.043
• 发表时间: 2008-04-15
• 期刊: JOURNAL OF THE NEUROLOGICAL SCIENCES
• 影响因子: 4.4
• 作者: Sun, Guilian;Haginoya, Kazuhiro;Tsuchiya, Shigeru
• 通讯作者: Tsuchiya, Shigeru

The analysis of the functions of human B andT cells in humanizedNOD/shi-scid/gammac(null) (NOG) mice (hu-HSC NOG mice).

人源化NOD/shi-scid/gammac(null) (NOG)小鼠（hu-HSC NOG小鼠）中人B细胞和T细胞的功能分析。

• 发表时间: 2009
• 期刊: Int.Immunol. 21
• 作者: Watanabe Y;Takahashi T;Okajima A;Shiokawa M;Ishii N;Katano I;Ito R;Ito M;Minegishi M;Minegishi N;Tsuchiya S;Sugamura K.
• 通讯作者: Sugamura K.