Quality assessment of bone and surface modification of dental implant

本研究的最终目的是建立牙种植体周围骨质量评价方法和牙种植体表面改性方法。以钛板为载体培养的成骨细胞作为种植体周围骨质量评价的模型。对成骨细胞MC 3 T3-E1在钛板上培养形成的细胞外基质进行了结构观察和^3H-脯氨酸定量分析。用RT-PCR方法检测MC 3 T3-E1细胞在钛片上骨钙素、骨连接素、骨桥蛋白、骨涎蛋白和cbfa-1的mRNA表达。因此，明确了骨的细胞外基质是通过培养的成骨细胞在钛上随时间钙化而形成的，并且成骨细胞MC 3 T3-E1细胞表达作为成骨细胞标志物的mRNA。这些结果表明，RT-PCR基因表达分析和^3H-脯氨酸组织培养细胞外基质定量分析可用于临床上对患者种植区周围骨质量的评估。结果表明，SiC处理钛促进钙化，钛的钙化是细胞外基质与钛结合的重要因素。因此，SiC处理法和细胞外基质结合法是提高钛表面生物相容性的有效方法。在本报告中，以下六章详细解释了这些结果。（第1、2章：骨质量评估，第3、4章：钛表面改性，第5、6章：种植体临床应用）

Final objects in the present study are the development of quality assessment of bone around dental implant area and surface modification of dental implant. Characteristics of osteoblasts cultured on titanium plate were analyzed as a model of quality assessment of bone around dental implant area. The structural observation and the quantitative analysis with ^3H-proline of extracellular matrix formed by osteoblastic MC3T3-E1 cell cultured on titanium plate were curried out. Still more, the mRNA expression of the MC3T3-E1 cells cultured on titanium for osteocalcin, osteonectine, osteopontin, bone sialoprotein and cbfa-1 was analyzed with RT-PCR method. Consequently, it was clarified that extracellular matrix of bone was formed by cultured osteoblast to calcify with time on titanium and the osteoblastic MC3T3-E1 cell expressed mRNA as osteoblastic marker. These results suggested that RT-PCR analysis for gene expression and quantitative analysis for extracellular matrix in tissue culture with ^3H-proline were utilized for development of quality assessment of bone around dental implant area for patients in clinical field.Then, in order to develop titanium with high bio-compatibility as biomaterial, some methods for surface modification of titanium were examined. The results indicated that treatment titanium with SiC promoted calcification and calcification of titanium was important for combining extracellular matrix with titanium. Therefore, the SiC treatment method and the combination method of extracellular matrix were developed as effective method for upgrading bio-compatibility of surface of titanium. In the present reports, these results were explained in details in following six chapters. (Chapter 1, 2: quality assessment of bone, Chapter 3, 4: surface modification of titanium, Chapter 5, 6: clinical application for implant)