Influence of osteoporosis and aging in the bone structure to biomaterial

骨质疏松和骨结构老化对生物材料的影响

• 批准号: 10671831
• 负责人: ICHIKAWA Tetsuo
• 金额: $ 2.37万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671831/
• 关键词: dental implant; bone-implant interface; hydroxyapatite; titanium; osteoporosis; 老齢; インプラント; インプラント周囲骨; アパタイト

The aim of this experimental study was to evaluate the influence of osteoporosis induced after implantation on bone structure around dental implants using ovariectomized rats. Tibiae of 30 to 32-old female Wistar rats were used in this study. Hydroxyapatite (HA) and titanium (Ti) rods were inserted into tibiae of 6 4 rats. Six weeks after implantation, 28 rats were ovariectomized (ovx), 26 rats were sham-operated (sham) and 10 rats were sacrificed (control). Four, 8 and 16 weeks following the operation, the rats were sacrificed. The reaction of the bone tissue surrounding HA and Ti implants was examined histologically. The results can be summarized as follows.1) The body weight of "ovx" rats markedly increased compared with "sham" rats. Trabecular bone volume percent in distal region of femurs of "ovx" was significantly lower than that of "sham" 4, 8 and 16 weeks following the operation. These findings showed that the this model represents osteoporosis.2) Mature trabecular bone around HA in "ovx" was resorbed chronologically, but some remained at 16 weeks. In "sham", a large amount of trabecular bone remained around the HA and was connected to the cortical bone as seen m "control" The area of connective tissue increased chronologically m the HA and bone interface. Sixteen weeks following the operation, fibrous tissue was observed on the implant surface.3) Very small amount of mature trabecular bone remained around Ti in "ovx" as seen in distal region of femurs, whereas the thickness of surrounding bone rapidly increased at 1 6 weeks. A greater amount of mature bone was found close to Ti m "sham" compared to that in "ovx". These findings suggest that the bone reaction, especially in the bone-implant interface, is different between HA and Ti in cases of osteoporosis.

本实验以去卵巢大鼠为实验对象，探讨种植后骨质疏松对种植体周围骨结构的影响。本研究以30 ~ 32岁雌性Wistar大鼠胫骨为研究对象。采用羟基磷灰石（HA）和钛（Ti）棒植入64只大鼠胫骨。植入6周后，切除卵巢28只，假手术26只，处死10只（对照组）。术后4、8、16周处死大鼠。组织学观察了羟基磷灰石和钛植入物周围骨组织的反应。结果可以总结如下。1)“ovx”大鼠的体重较“sham”大鼠明显增加。术后4、8、16周，“ovx”组股骨远端骨小梁体积百分比明显低于“sham”组。这些发现表明该模型代表骨质疏松症。2)卵形骨HA周围的成熟骨小梁骨按时间顺序被吸收，但在16周时仍有部分骨小梁骨被吸收。在“假手术”中，大量骨小梁骨保留在HA周围并与皮质骨相连，如“对照组”中所见，结缔组织面积在HA和骨界面上按时间顺序增加。术后16周，种植体表面可见纤维组织。3)股骨远端“ovx”区Ti周围仍有少量成熟骨小梁，16周时周围骨厚度迅速增加。与“ovx”相比，在靠近Ti m“sham”的地方发现了更多的成熟骨。这些发现表明，骨质疏松症患者的骨反应，特别是在骨-种植体界面，在HA和Ti之间是不同的。

项目成果

川本苗子: "骨粗鬆症ラットにおけるインプラント周囲骨組織の経時的変化-インプラント埋入後に骨粗鬆症を発症した場合-"四国歯学会雑誌. 12・1. 173-188 (1999)

Naeko Kawamoto：“骨质疏松大鼠种植体周围骨组织的时间变化 - 种植体植入后发生骨质疏松症 -”四国牙科学会杂志 12・1（1999）。

川本苗子: "骨粗鬆症ラットにおけるインプラント周囲骨組織の経時的変化-インプラント埋入後に骨粗鬆症を発症した場合-"四国歯学会雑誌. 12. 173-188 (1999)

Naeko Kawamoto：“骨质疏松大鼠种植体周围骨组织的时间变化 - 种植体植入后发生骨质疏松症 -” 四国牙科学会杂志 12. 173-188 (1999)。

Naeko Kawamoto: "Chronological change in the bone structure around implants in rats with osteoporosis : a case of osteoporosis induced after implantation"Shikoku Dental Research. 10(1). 1-10 (1997)

Naeko Kawamoto：“骨质疏松症大鼠植入物周围骨结构的时间变化：植入后诱发骨质疏松症的案例”四国牙科研究。