Effect of physical factors on osseointegration

物理因素对骨整合的影响

• 批准号: 14370637
• 负责人: ICHIKAWA Tetsuo
• 金额: $ 4.99万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370637/
• 关键词: mechanical stress; compressing force; magnetic field; micro pulse magnetic field; Cox2; cDNA microarray; Hsp25; immune reaction; 免疫応答; 骨芽細胞; 細胞外マトリクス; 骨形成; Osseointegration; 物理的因子; 磁場; in vitro; in vivo; 組織; リモデリング

It is well known that appropriate occlusal force promotes bone formation around implants, whereas bone atorophy and bone resorption develop under no mechanical stress or overstressing. It has been reported that when shear stress loads to osteoblasts, increase of intracellular Ca^<2+> concentration, activation of protein kinase A, and upregulation of cyclooxygenase 2 (Cox-2) gene result in differentiation of osteoblasts. The purpose of this study was to clarify appropriate levels of stress for the bone remodeling and mechanotransduction signaling at molecular levels.We examined pattern of gene expression when compressing force was directly loaded to a mouse osteoblast like cell line (MC3T3-E1) for 24 hours by reverse transcription polymerase chain reaction and cDNA macroarray. Furthermore, we examined change of gene expression under its stress by cDNA microarray.Cox-2, c-fos, and alkaline phosphatase mRNAs were upregulated in proportion to the stress value when compressing force was loaded. The analysis of cDNA macroarray showed that expression of bone morphogenetic protein 4 (BMP4) and BMP7, which promote differentiation of osteoblasts, was upregulated. No differences of survival of MC3T3-E1 cells were observed between loading and unloading of stress. Furthermore, the analysis of cDNA microarray showed that compressing force upregulated expression of heat shock protein 25kDa (Hsp25). And when MC3T3-E1 cells were cultured in magnetic field and micro pulse magnetic field, Cox-2, c-fos, and alkaline phosphatase mRNAs were upregulated.The present study indicates that the compression force promotes differentiation of MC3T3-E1 cells through the signaling pathway upregulating Hsp25.In the study of immune reaction of dendritic cells to titaniumtitanium could not induce the immune reactions easily. It may be caused by the interface of Ti and immune cells which are ‘static' environment.

众所周知，适当的咬合力会促进种植体周围的骨形成，而在没有机械应力或过度应力的情况下会发生骨萎缩和骨吸收。据报道，当剪切应力负载至成骨细胞时，细胞内Ca 2+ 浓度增加、蛋白激酶A活化和环氧合酶2(Cox-2)基因上调导致成骨细胞分化。本研究的目的是在分子水平上阐明骨重塑和力转导信号传导的适当压力水平。我们通过逆转录聚合酶链反应和 cDNA 宏阵列检查了将压缩力直接加载到小鼠成骨细胞样细胞系 (MC3T3-E1) 24 小时时的基因表达模式。此外，我们通过cDNA微阵列检测了应激下基因表达的变化。当施加压力时，Cox-2、c-fos和碱性磷酸酶mRNA与应激值成比例上调。 cDNA宏阵列分析显示，促进成骨细胞分化的骨形态发生蛋白4（BMP4）和BMP7的表达上调。在加载和卸载应力之间没有观察到 MC3T3-E1 细胞的存活率差异。此外，cDNA微阵列分析表明，压力上调热休克蛋白25kDa（Hsp25）的表达。并且当MC3T3-E1细胞在磁场和微脉冲磁场中培养时，Cox-2、c-fos和碱性磷酸酶mRNA上调。本研究表明，压力通过上调Hsp25的信号通路促进MC3T3-E1细胞的分化。在树突状细胞对钛的免疫反应的研究中，钛不能轻易诱导免疫反应。这可能是由于钛与免疫细胞的界面处于“静态”环境所致。

项目成果

New Magnetic Applications in Clinical Dentistry (Minoru Ai, Uuh-Yuan Shiau)

临床牙科中的新磁性应用（Minoru Ai、Uuh-Yuan Shiau）

• 发表时间: 2004
• 期刊: Biological Effects of Magnetic Attachment on the Human Body and Tissue (Quintessence books)
• 作者: Tetsuo Ichikawa;Naeko Kawamoto
• 通讯作者: Naeko Kawamoto

光造形法 第3章 症例から見るインプラント技工の実際 4 即時インプラント補綴法 1)光造形法

立体光刻术 第 3 章 从案例看实际种植技术 4 即刻种植修复体 1) 立体光刻术

• 发表时间: 2004
• 期刊: 歯科技工別冊 2004インプラントの技工
• 作者: 友竹偉則;市川哲雄
• 通讯作者: 市川哲雄

New Magnetic Applications in Clinical Dentistry(Minoru Ai, Uuh-Yuan Shiau) Biological Effects of Magnetic Attachment on the Human Body and Tissue(44-47)

磁性在临床牙科中的新应用（Minoru Ai，Uuh-Yuan Shiau）磁性附着对人体和组织的生物效应（44-47）

• 发表时间: 2004
• 作者: Tetsuo Ichikawa;Naeko Kawamoto
• 通讯作者: Naeko Kawamoto

New Magnetic Application in Clinical Dentistry (Minoru Ai, Uuh-Yuan Shiau) Biological Effects of Magnetic Attachment on the Human Body and Tissue (44-47)

临床牙科中的新磁性应用（Minoru Ai，Uuh-Yuan Shiau）磁性附着对人体和组织的生物效应（44-47）

• 发表时间: 2004
• 作者: Tetuo Ichikawa;Naeko Kawamoto
• 通讯作者: Naeko Kawamoto

In vitro Study of Stress Distribution on Additional Posterior Mandibular Implants

附加下颌后牙种植体应力分布的体外研究

• 发表时间: 2004
• 期刊: Prosthodontic Research & Practice 3
• 作者: Wei Hua;Tomotake Yoritoki;Nagao Kan;Oguri Takafumi;Nagao Daisuke;Ichikawa Tetsuo
• 通讯作者: Ichikawa Tetsuo