权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

cancer pain and neuropathic pain in orofacial region

口面部癌性疼痛和神经性疼痛

基本信息

批准号：
12470445
负责人：
NAKANISHI Osamu
金额：
$ 5.18万
依托单位：
Kyushu Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470445/
关键词：
Cancer pain Neuropathic pain hyperalgesia CSF-glutamate c-fos apoptosis 脳脊髄液中glutamate

项目摘要

This study was to investigated the manifestation of injury characteristics of a specific nerve cell of a trigeminal spinal tract nucleus (c-fos manifestation -apoptosis) and the therapy about cancer and neuropathic pain in a orofacial region.Hyperalgesia for pain sensation to lower lip and glutamate level in cerebrospinal fluid (HPLC-ECD method) were examined in cancer (lytic tumor cell injection to maxillary bone) and neuropathic pain (silicon injection in nurvus alveolar mandibular pipe). For histopathological examination, a trigeminal spinal tract nucleus were removed and frozed up and made a tissue graft (neuropathic pain model : after 5, 10th days, cancer pain model : after 20, 25th days).For these tissue grafts, c-fos gene expression (in situ hybridization method) and TUNEL dyeing were done to make clear for relation to programmed cell death.Hyperalgesia was appeared from 3rd to 25th day in the neuropathic pain model, and from 18td to 25th day in the cancer pain model.As for the glutamate level incerebrospinal fluid, increase 20% was recognized in the neuropathic pain model on the 10th day, and more than 30% increase in cancer pain model on the 20th day.And in a histopathological change of a trigeminal spinal tract nucleus, the increase of c-fos protein was appeared on 5th, and increase of necrosis was recognized on 10th days in the neuropathic pain model.Increase of c-fos protein and necrosis were acceptation the 20th day in a cancer pain model.Administration of N type Ca 2+ channel inhibitors or 5-HT2A inhibitor inhibited the decline of pain threshold and increase of c-fos protein and necrosis were restrained in the neuropathic pain model.Above this, programmed cell death by cancer pain and neuropathic pain were become clear, and N type Ca2+ channel inhibitors and 5-HT2A inhibitor have a effects, and be shown the directionality of a therapy in cancer pain and neuropathic pain.

本研究旨在探讨三叉神经脊束核特定神经细胞损伤特征的表现（c-fos表现-细胞凋亡）与口面部肿瘤和神经病理性疼痛的治疗。下唇痛觉过敏与脑脊液谷氨酸水平采用高效液相色谱-电子捕获检测法（HPLC-ECD）检测癌性（上颌骨溶解性肿瘤细胞注射）和神经性疼痛（下颌牙槽管内硅注射）患者的血清中抗肿瘤活性。组织病理学检查，取三叉神经脊束核，冷冻后制成组织移植物（神经病理性疼痛模型：5、10天后，癌痛模型：20、25天后）。对于这些组织移植物，c-fos基因表达（原位杂交法）神经病理性痛模型在第3 ~ 25天出现痛觉过敏，在神经病理性疼痛模型中，脑脊髓液中谷氨酸水平在第10天增加20%，在癌痛模型中在第20天增加超过30%。在三叉神经脊束核的组织病理学变化中，c-fos蛋白表达于第5天开始增加，神经病理性疼痛模型在第10天出现坏死，癌性疼痛模型在第20天出现c-fos蛋白增加和坏死，应用N型钙通道抑制剂或5-HT 2A抑制剂可抑制神经病理性痛模型痛阈的下降和c-fos蛋白的增加，抑制神经病理性痛模型的坏死，在此基础上，癌性痛和神经病理性痛引起的程序性细胞死亡变得明显，N型Ca 2+通道抑制剂和5-HT 2A抑制剂具有一定的作用，并显示出癌症疼痛和神经性疼痛治疗的方向性。