Reproduction and reconstruction of a medulla spinals sensory cell at the cancer and neuropathic pain in orofacial region

本文从谷氨酸毒性、局部c-fos改变和神经可塑性神经元损伤特征、三叉神经节痛觉信息的输入和/或控制系统等方面综述神经病理性痛和癌性痛的机制。癌症和神经性疼痛产生注射癌细胞（Walker 256 B癌）骨膜下注射，并在大鼠下颌管注射硅。此外，将环型微透析探针从枕大池插入蛛网膜下腔，探针尖端位于三叉神经核水平。1）行为：2）采用透析探针法获得透析液，用HPLC-ECD法测定透析液中谷氨酸的浓度。制备肿瘤和神经病理性疼痛模型后，取三叉神经脊束核，冰冻切片，原位杂交检测c-fos基因表达。在痛觉刺激时三叉神经脊束核部脑螺旋液谷氨酸含量的变化方面，在癌痛和神经病理性疼痛大鼠中显著增加。在病理检查中，肿瘤和神经病理性疼痛大鼠三叉神经脊束核尾侧亚核早期就有c-fos基因表达。综述：该基因表达在疼痛引起的程序性细胞死亡之前，并对损伤后的突触再生能力进行了综述。并对临床应用中存在的问题和有待改进的地方进行了探讨。我们相信，建立一个高可能性的治疗方法可以使它得到更大的发展。

This study was reviewed the mechanism of neuropathic and cancer pain from the glutamate toxicity, a local change of c-fos and neuroplasticity in neuronal damage characteristics, and the input and/or control system in trigeminal ganglion of algesthesia informationMethod: Cancer and neuropathic pain was produced to inject a cancer cell (Walker256B carcinocarcinoma) in a subperiosteum, and to inject silicon in a mandibular canal of rat, respectively. In addition, loop type microdialysis probe was inserted in cavum subarachnoideale from cisterna magna go that tip was located in trigeminal nuclei level.1) Behavior: The algetic behavior were evaluated by the counts of reaction of clinching in a hot box.2) It were measured the glutamate concentration of the dialysate which it obtained using dialysis probe by HPLC-ECD method in cancer and neuropathic pain.3) After cancer and neuropathic pain model preparation, it delivered nucleus tractus spinalis nervi trigemini and made frozen section and reviewed c-fos gene expression by in situ hybridization method.Results: It showed rat of the cancer and the neuropathic pain shortening that pain latency was significant both. As for the alteration of glutamate of the cerebral spiral fluid of nucleus tractus spinalis nervi trigemini part in algetic irritation, it increase significantly in a cancer and neuropathic pain rat. In pathological examination, cancer and neuropathic pain rat was found c-fos gene expression from early stage in caudalis subnuclei of nucleus tractus spinalis nervi trigemini.Summary : It review the gene expression to precede programmed cell death by pain and review synapse regenerative power of lesion. And problems and the point that could be improved became clear toward clinical application. We are convinced that establishment of a high therapy of likelihood can develop it more.