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The elucidation of the reconstruction mechanism of the nucleus tractus spinalis sensory nerve in region of face cancer pain and the neuropathic pain

面癌痛及神经病理性疼痛区域棘束核感觉神经重建机制的阐明

基本信息

批准号：
20592377
负责人：
NAKANISHI Osamu
金额：
$ 2.83万
依托单位：
Kyushu Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2010
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592377/
关键词：
癌性疼痛神経因性疼痛グリア細胞再生

项目摘要

In this study, we elucidated effect of the spinal nerve-glia interaction on cancer pain and/or neuropathic pain of the rat regio infraorbitalis and studied it for the purpose of obtaining an underlying finding whether or not the treatment by the neurotrophy factor was effective. At first we made the inflammatory pain and cancer pain rat model that injected Freund's adjuvant or Walker 256 B into the rat regio infraorbitalis and gave antiphlogistic indomethacin to an inflammatory pain rat and a cancer pain rat to determine difference in both pain. Furthermore, we determined manifestation by the early period of onset of the c-fos gene in the merotomy of the neuropathic pain and the cancer pain rat. The pain behavior significantly inhibited it with each drug when COX-II inhibitor, p38MAPK inhibitor, microglial inactivator were given to the neuropathic pain and the cancer pain rat, respectively. In the neuropathic pain, activation of the spinal cord glutamate nervous system occurs early in … More the onset, and activation of p38-MAPK occurs successively, Synaptic transmission of the spinal nerre system was modified remarkably, and the microglia which was inflammation charge cells nearby activated it, and what it integrated it to be accompanied with COX-II, activation and BDNF (brain-derived neurotrophic factor) isolation of p38-MAPK, and developed in neuropathic pain became clear. We performed immunostaining of the trigeminal ganglion merotomy in GFAP, Iba1 to examine the microglia and astrocytic in neuropathic pain and the cancer pain rat contribution, respectively. Microglial activation was found early in the pain onset in both of the examination of the neuropathic pain and the cancer pain, and astrocytic activation was found late. Also, a significant decrease of cancer pain was found when subcutaneous injection of the microglial inactivator to a cancer pain rat was provided. By this result, It was found that spinal cord - microglial interaction was involved in both pain mechanisms of neuropathic pain and cancer pain. Less

在这项研究中，我们阐明了脊髓神经-胶质细胞相互作用对大鼠眶下区癌性疼痛和/或神经病理性疼痛的影响，并研究了它的目的是获得一个潜在的发现，无论是治疗神经营养因子是有效的。首先，我们制作了炎性疼痛和癌症疼痛大鼠模型，将弗氏佐剂或步行者256 B注射到大鼠眶下区，并对炎性疼痛大鼠和癌症疼痛大鼠给予消炎痛以确定两者疼痛的差异。此外，我们确定了表现的早期发病的c-fos基因在神经病理性疼痛和癌痛大鼠的分切。COX-II抑制剂、p38 MAPK抑制剂、小胶质细胞灭活剂分别对神经病理性痛和癌痛大鼠的痛行为有明显的抑制作用。在神经病理性疼痛中，脊髓谷氨酸神经系统的激活发生在脊髓损伤的早期。 ...更多信息 p38-MAPK的启动和激活先后发生，脊髓神经系统的突触传递明显改变，附近的小胶质细胞作为炎性电荷细胞激活了它，它整合了它，伴随着COX-Ⅱ，p38-MAPK的激活和BDNF（脑源性神经营养因子）的分离，并在神经病理性疼痛中发展。我们对三叉神经节切断处的胶质纤维酸性蛋白（GFAP）、Iba 1进行了免疫组化染色，以分别检测小胶质细胞和星形胶质细胞在神经病理性疼痛和癌性疼痛大鼠中的贡献。在神经病理性疼痛和癌痛的检查中，发现小胶质细胞活化在疼痛发作的早期，而星形胶质细胞活化被发现较晚。此外，当向癌痛大鼠皮下注射小胶质细胞灭活剂时，发现癌痛显著减轻。结果表明，脊髓-小胶质细胞的相互作用参与了神经病理性疼痛和癌痛的疼痛机制。少