IMMUNOBIOLOGICAL STUDIES ON ORAL LESION OF SJOGREN'S SYNDROME

干燥综合征口腔病变的免疫生物学研究

• 批准号: 12470448
• 负责人: FUJIBAYASHI Takashi
• 金额: $ 6.53万
• 依托单位: Dokkyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470448/
• 关键词: Sjogren's syndrome; revised diagnostic criteria; number of infiltrating lymphocytes; IPAP-Win; MR-sialography; salivary secretion rate; chewing gum test; Saxon's test; 画像解析装置; focus; 口腔乾燥症; 診断基準; 1次性シェーグレン症候群; 2次性シェーグレン症候群; MR-Sialography

Recent studies indicate that 120kl) α-fodrin has been estimated as the autoantigen in NFS/sld model mouse which seems to be the disease model of the Sjogren's syndrome. In this study, the reaction with the serum of Sjogren's syndrome (SS) patient was planned by Western blot method of refined α-fodrin and human recombinant α-fodrin. However, we could get the sufficient result because of the limited number of the material. The result of various examinations in diagnosing SS was compared between primary SS and secondary SS diagnosed by revised Japanese criteria (1999) in 120 cases. No significant difference was observed between them. Therefore, it is suggested that both primary and secondary SS can be diagnosed by the same diagnostic criteria. As for the diagnostic accuracy of facilities were examined. The study showed 96.0% in sensitivity and 90.5% in speificity. The diagnostic accuracy was as high as 94.5%.The quantitative measurement of the infiltrating lymphocyte was made by the IPAP-Win system in which the microscope was linked with the personal computer in 125 samples of labial salivary glands biopsies from 25 SS patients. Average area of a labial gland was 2.77mm^2, that of a lobulus was 0.55mm^2, that of a focus was 0.0638mm^2, and average lymphocyte number in a focus was 345. The MR-sialography is non-invasive method of imaging without the insertion of catheter and the contrast media. The usefulness of MR-sialography was examined by comparing with the conventional X-ray sialography. There was a highly significant correlation between the two methods (rcc=0.885, p<0.0001), and MR-sialography may be able to replace the conventional method. The revised Japanese criteria include sialometry in which either the chewing gum test or the Saxon test is adopted. Studies on the chewing gum test were performed by investigating the reproducibility, the cutoff value in diagnosing as hyposalivation, and the correlation between the chewing gum test and the Saxon test.

近年来的研究表明，NFS/sld模型小鼠的自身抗原可能是120 kl α-fodrin，而NFS/sld模型小鼠可能是干燥综合征的疾病模型。本研究采用Western blot方法，设计了精制α-胞衬蛋白和重组人α-胞衬蛋白与干燥综合征（SS）患者血清的反应。然而，由于材料的有限性，我们可以得到充分的结果。本文对120例原发性SS和继发性SS患者的各项检查结果进行了比较。两组间无显著性差异。因此，认为原发性和继发性SS可以用相同的诊断标准进行诊断。至于诊断的准确性设施进行了检查。本法敏感性96.0%，特异性90.5%。应用IPAP-Win系统对25例SS患者的125份唇腺活检标本进行了浸润淋巴细胞的定量检测。唇腺平均面积为2.77 mm^2，小叶平均面积为0.55 mm^2，病灶平均面积为0.0638 mm^2，病灶内平均淋巴细胞数为345个。磁共振涎腺造影是一种无创的成像方法，无需插入导管和造影剂。通过与常规X线涎腺造影的比较，验证了MR涎腺造影的有效性。两种方法之间有高度显著相关性（rcc=0.885，p<0.0001），MR涎腺造影可能能够取代传统方法。修订后的日本标准包括唾液腺测量法，其中采用口香糖试验或Saxon试验。对咀嚼胶试验的重复性、诊断少涎的临界值以及咀嚼胶试验与Saxon试验的相关性进行了研究。

项目成果

藤林孝司: "口腔粘膜疾患"Modern Physician. 21・8. 1169-1173 (2001)

藤林隆：《口腔粘膜疾病》《现代医师》21・8（2001）。

藤林孝司: "口腔・歯科の免疫学入門"クインテッセンス出版. 143 (2000)

Takashi Fujibayashi：“口腔和牙科免疫学导论”Quintessence Publishing 143（2000）。

武田真由美, 藤林孝司, 渡辺八州郎: "Sjogren症候群の診断におけるMR-sialographyの検討"日本口腔粘膜学会雑誌. 8. 58-67 (2002)

Mayumi Takeda、Takashi Fujibayashi、Yashuro Watanabe：“干燥综合征诊断中的 MR 唾液造影研究”日本口腔粘膜学会杂志 8. 58-67 (2002)。

T.Fujibayashi: "Xerostomia and other oral problems in the menopause,"The Menopause at the Millennium""The Parthenon Publishing Group. 157-161 (2000)

T.Fujibayashi：“更年期的口干症和其他口腔问题”，《千年的更年期》，帕台农神庙出版集团。

藤林 孝司: "シェーグレン症候群の国際診断基準の討論のために"シェーグレン症候群へのStrategy. No.2. 49-57 (2003)

Takashi Fujibayashi：“关于干燥综合征国际诊断标准的讨论”干燥综合征策略，第 2. 49-57 号（2003 年）。