IMMUNOBIOLOGICAL STUDIES ON ORAL LESION OF SJOGREN'S SYNDROME

最近的研究表明，120kL的α-fodrin被认为是干燥综合征模型小鼠的自身抗原。本研究采用精制α-Fodrin和人重组α-Fodrin的Western印迹方法，设计与干燥综合征(SS)患者血清的反应计划。然而，由于材料的数量有限，我们可以得到足够的结果。对120例按日本修订标准(1999)诊断的原发性SS和继发性SS的各种检查结果进行了比较。两组间无显著差异。因此，原发和继发性SS可以用相同的诊断标准进行诊断。对于设备的诊断准确性进行了检查。研究表明，敏感性为96.0%，特异性为90.5%。用IPAP-WIN系统对25例SS患者的125例唇涎腺活检标本进行了定量检测。平均唇腺面积为2.77 mm^2，小叶面积为0.55 mm^2，病灶面积为0.0638 mm^2，平均淋巴细胞数为345个。MR涎腺造影术是一种不需要插入导管和造影剂的非侵入性成像方法。通过与常规X线唾液管造影术的比较，探讨MR涎腺造影术的应用价值。两种方法之间存在高度显著的相关性(RCC=0.885，p&lt；0.0001)，MR涎管造影术可能能够取代传统的方法。修订后的日本标准包括唾液测定法，其中要么采用口香糖试验，要么采用撒克逊试验。通过考察口香糖试验的重复性、口香糖试验与撒克逊试验之间的相关性以及口香糖试验在诊断肺功能低下中的临界值，对口香糖试验进行了研究。

Recent studies indicate that 120kl) α-fodrin has been estimated as the autoantigen in NFS/sld model mouse which seems to be the disease model of the Sjogren's syndrome. In this study, the reaction with the serum of Sjogren's syndrome (SS) patient was planned by Western blot method of refined α-fodrin and human recombinant α-fodrin. However, we could get the sufficient result because of the limited number of the material. The result of various examinations in diagnosing SS was compared between primary SS and secondary SS diagnosed by revised Japanese criteria (1999) in 120 cases. No significant difference was observed between them. Therefore, it is suggested that both primary and secondary SS can be diagnosed by the same diagnostic criteria. As for the diagnostic accuracy of facilities were examined. The study showed 96.0% in sensitivity and 90.5% in speificity. The diagnostic accuracy was as high as 94.5%.The quantitative measurement of the infiltrating lymphocyte was made by the IPAP-Win system in which the microscope was linked with the personal computer in 125 samples of labial salivary glands biopsies from 25 SS patients. Average area of a labial gland was 2.77mm^2, that of a lobulus was 0.55mm^2, that of a focus was 0.0638mm^2, and average lymphocyte number in a focus was 345. The MR-sialography is non-invasive method of imaging without the insertion of catheter and the contrast media. The usefulness of MR-sialography was examined by comparing with the conventional X-ray sialography. There was a highly significant correlation between the two methods (rcc=0.885, p<0.0001), and MR-sialography may be able to replace the conventional method. The revised Japanese criteria include sialometry in which either the chewing gum test or the Saxon test is adopted. Studies on the chewing gum test were performed by investigating the reproducibility, the cutoff value in diagnosing as hyposalivation, and the correlation between the chewing gum test and the Saxon test.