Design of Artificial Cell Membrane Interface and Development of Evaluation System for Biorecognition Function
人工细胞膜界面设计及生物识别功能评价系统开发
基本信息
- 批准号:12554029
- 负责人:
- 金额:$ 8.7万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2000
- 资助国家:日本
- 起止时间:2000 至 2001
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
To develop an anion sensing system in water based on a chromoionophore/artificial cell membrane conjugate, novel thiourea-based chromoionophores having various lengths of alkyl chains (Cn-TU) were designed and their anion recognition function in aqueous vesicle solutions were evaluated. This study revealed that the positioning of chromophore binding sites inside the didodecyldimethylammonium bromide (DDAB) vesicle was successfully controlled by the alkyl chain length of Cn-TU. The cationic vesicle interface was found to exhibit a filter function for the less hydrophobic anions, and highly selective anion recognition via hydrogen bonding was achieved by C1-TU located deep inside the DDAB vesicle. Function of crown ether chromoionophores for alkali metal ion recognition in micellar system were also examined. The surface charge of the micelle was found to strongly influence the ion selectivity and sensitivity, and selective Na^+ recognition was only performed by anionic micellar system. To evaluate ion recognition at these membrane interfaces, a resonant second harmonic generation (SHG) analysis system was developed by using 15-crown-5 azophenol as a model probe. Selective sorption of alkali metal complexes with the azoprobe at heptane/water interface was confirmed by SHG spectra, and their conformation changes upon metal recognition at the interface were successfully evaluated by SHG analysis.
设计了一种新型的不同烷基链长的硫脲基色离子载体(CN-TU),并对其在水泡溶液中的阴离子识别性能进行了评价。研究表明,CN-TU的烷基链长成功地控制了发色团结合部位在DDAB囊泡中的定位。阳离子囊泡界面对疏水性较弱的阴离子具有过滤作用,位于DDAB囊泡深处的C1-TU通过氢键实现了对阴离子的高选择性识别。考察了冠醚发色团在胶束体系中对碱金属离子的识别作用。胶束的表面电荷对离子的选择性和灵敏度有很大影响,只有阴离子胶束体系才能选择性地识别Na~+。为了评价离子在这些膜界面上的识别能力,以15-冠-5-偶氮苯酚为模型探针,建立了共振二次谐波分析系统。SHG谱证实了偶氮染料与碱金属络合物在庚烷/水界面上的选择性吸附,并通过SHG分析评价了它们在界面金属识别过程中的构象变化。
项目成果
期刊论文数量(26)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
T. Hayashita, T. Onodera, R. Kato, S. Nishizawa, N. Teramae: "Positioning Dependent Anion Recognition by Thiourea-Based Chromoionophores via Hydrogen Bonding in Vesicle Solution"Chem. Commun.. 755-756 (2000)
T. Hayashita、T. Onodera、R. Kato、S. Nishizawa、N. Teramae:“基于硫脲的发色离子载体通过囊泡溶液中的氢键进行定位依赖性阴离子识别”Chem。
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- 影响因子:0
- 作者:
- 通讯作者:
T.Hayashita,T.Onodera,S.Nishizawa,N.Teramae, et al.: "Positioning Dependent Anion Recognition by Thiourea-Based Chromoionophores via Hydrogen Bonding in Aqueous Vesicle Solution"Chemical Communications. 755-756 (2000)
T.Hayashita、T.Onodera、S.Nishizawa、N.Teramae 等人:“基于硫脲的发色离子载体通过水性囊泡溶液中的氢键进行定位依赖性阴离子识别”化学通讯。
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- 影响因子:0
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A.Yamauchi,T.Hayashita: "Design and Function of Metal Ion Probes Based on Photosignal Transduction"Bunseki Kagaku. 49. 75-89 (2000)
A.Yamauchi,T.Hayashita:“基于光信号转导的金属离子探针的设计和功能”Bunseki Kagaku。
- DOI:
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- 影响因子:0
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- 通讯作者:
T.Hayashita, T.Onodera, R.Kato, N.Teramae et al.: "Positioning Dependent Anion Recognition by Thiourea-Based Chromoionophores via Hydrogen Bonding in Vesicle Solution"Chem.Commun.. 755-756 (2000)
T.Hayashita、T.Onodera、R.Kato、N.Teramae 等人:“基于硫脲的发色离子载体通过囊泡溶液中的氢键进行定位依赖性阴离子识别”Chem.Commun. 755-756 (2000)
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- 影响因子:0
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A.Tong,T.Hayashita,B.D.Smith,N.Teramae, et al.: "Boronic Acid Fluorophore/β-Cyclodextrin Complex Sensors for Selective Sugar Recognition in Water"Analytical Chemistry. (印刷中). (2001)
A. Tong、T. Hayashita、B. D. Smith、N. Teramae 等人:“用于选择性识别水中糖的硼酸荧光团/β-环糊精复合传感器”(出版中)。
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HAYASHITA Takashi其他文献
Design of Saccharide Recognition Material Based on Boronic Acid Fluorophore/Cyclodextrin Gel
基于硼酸荧光团/环糊精凝胶的糖类识别材料的设计
- DOI:
10.5182/jaie.29.126 - 发表时间:
2018 - 期刊:
- 影响因子:0
- 作者:
SOMA Suzuka;SUZUKI Takahito;G. BEKELE Tewodros;TSUCHIDO Yuji;HASHIMOTO Takeshi;HAYASHITA Takashi - 通讯作者:
HAYASHITA Takashi
Fluorescence Detection of Endotoxin Using Cadmium Complexed with Dipicolylamine-type Pyrene Probe
镉与二吡啶胺型芘探针络合荧光检测内毒素
- DOI:
10.5182/jaie.33.95 - 发表时间:
2022 - 期刊:
- 影响因子:0
- 作者:
KIMOTO Hiroshi;IIYAMA Masamitsu;HASHIMOTO Takeshi;HAYASHITA Takashi - 通讯作者:
HAYASHITA Takashi
Synthesis and Self-Assembly of Oligosaccharide-Based Block Copolymers
低聚糖基嵌段共聚物的合成与自组装
- DOI:
- 发表时间:
2019 - 期刊:
- 影响因子:0
- 作者:
KITAMURA Aya;KASAI Yuna;TSUCHIDO Yuji;HASHIMOTO Takeshi;HAYASHITA Takashi;磯野拓也 - 通讯作者:
磯野拓也
Effect of Spacer Length in Pyrene-Modified-Phenylboronic Acid Probe/CyD Complexes on Fluorescence-based Recognition of Monosaccharides in Aqueous Solution
芘修饰苯硼酸探针/CyD 配合物中间隔基长度对水溶液中单糖荧光识别的影响
- DOI:
10.2116/analsci.20scp08 - 发表时间:
2021 - 期刊:
- 影响因子:1.6
- 作者:
TSUCHIDO Yuji;KOJIMA Shohei;SUGITA Ko;FUJIWARA Shoji;HASHIMOTO Takeshi;HAYASHITA Takashi - 通讯作者:
HAYASHITA Takashi
分子認識プローブ修飾ナノ粒子を用いた細菌検出法の開発
使用分子识别探针修饰纳米粒子开发细菌检测方法
- DOI:
- 发表时间:
2018 - 期刊:
- 影响因子:0
- 作者:
KITAMURA Aya;KASAI Yuna;TSUCHIDO Yuji;HASHIMOTO Takeshi;HAYASHITA Takashi;磯野拓也;土戸優志 - 通讯作者:
土戸優志
HAYASHITA Takashi的其他文献
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{{ truncateString('HAYASHITA Takashi', 18)}}的其他基金
Development of novel molecular recognition surface based on dendrimers
基于树枝状聚合物的新型分子识别表面的开发
- 批准号:
24655069 - 财政年份:2012
- 资助金额:
$ 8.7万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Development of Chromogenic Probe/Dendrimer Complexes for Molecular Discrimination
用于分子辨别的显色探针/树枝状聚合物复合物的开发
- 批准号:
21655030 - 财政年份:2009
- 资助金额:
$ 8.7万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Development of Molecular Imprinting Sensors Poccessing Sugar Recognition Function
具有糖识别功能的分子印迹传感器的开发
- 批准号:
18350043 - 财政年份:2006
- 资助金额:
$ 8.7万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Design of Supramolecular Cyclodextrin Complex Sensors and Construction of Functional Interfaces
超分子环糊精复合传感器的设计及功能接口的构建
- 批准号:
16550066 - 财政年份:2004
- 资助金额:
$ 8.7万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of Supramolecular Complex Sensor Possessing Biological Recognition Function
具有生物识别功能的超分子复合传感器的研制
- 批准号:
14340230 - 财政年份:2002
- 资助金额:
$ 8.7万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Design and Function of Molecular Complex Sensors Based on Supramolecular Structure
基于超分子结构的分子复合传感器的设计与功能
- 批准号:
12440208 - 财政年份:2000
- 资助金额:
$ 8.7万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular Design of Alkali Metal Ion Probes for Use in Aqueous Media
用于水介质的碱金属离子探针的分子设计
- 批准号:
09650893 - 财政年份:1997
- 资助金额:
$ 8.7万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
DEVELOPMENT OF NOVEL, POLYETHER CHELATING RESINS FOR USE IN SELECTIVE LEAD (II) SEPARATION
开发用于选择性铅 (II) 分离的新型聚醚螯合树脂
- 批准号:
07555265 - 财政年份:1995
- 资助金额:
$ 8.7万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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