Development of high sensitive electrochemical DNA microarray

高灵敏度电化学DNA微阵列的研制

• 批准号: 12557012
• 负责人: UCHIDA Kazuhiro
• 金额: $ 6.59万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12557012/
• 关键词: human genome; cDNA library; DNA chin; Micriarray; マウス; 電気化学

It is very important to develop a new DNA chip, which overcomes the problem in the present DNA microarray. In this project, we aim at developing a microelectrode, which immobilizes a synthetic oligonucleotide concerned with vanous genetic diseases and establishing a simple and quick electrochemical detection system for the hybrid with genomic DNA taken from patients. Once realized, this system will allow us to diagnose cancers and infectious diseases efficiently. We have investigated the followings: 1) Study of the preparation technology of microelectrode arrays, 2) Study of the probes of oligonucleotide on the electrode, 3) Study of a technology to analyze electrocheinical data, 4) Establishment of cDNA database for electrochemical array, 5) Study of preparation method of blood samples. We also analyzed genomic alterations in the bile duct cancers and thyroid cancers to identify the valuable genes for genomic diagnosis using electrochernical array. The knowledge thus obtained is useful to develop a potable gene diagnosis instrument to analyze genetic information.

因此，开发一种新的DNA芯片，克服现有DNA微阵列存在的问题，具有十分重要的意义。本课题旨在研制一种固定与多种遗传性疾病有关的人工合成寡核苷酸的微电极，并建立一种简单、快速的电化学检测系统，用于与患者基因组DNA杂交的检测。一旦实现，该系统将使我们能够有效地诊断癌症和传染病。本论文主要研究了以下几个方面：1）微电极阵列制备技术的研究; 2）微电极上寡核苷酸探针的研究; 3）电化学数据分析技术的研究; 4）电化学阵列cDNA数据库的建立; 5）血样制备方法的研究。我们还分析了胆管癌和甲状腺癌的基因组改变，以确定有价值的基因，基因组诊断使用电化学阵列。这些知识对研制便携式基因诊断仪进行遗传信息分析具有重要意义。

项目成果

Feng, R., Kabayama, A., Uchida, K, Hoshino, H., Miwa, M.: "Cell-free entry of human T-cell leukemia virus type 1 to mouse cells."Jpn.J.Cancer Res.. 92. 410-416 (2001)

Feng, R.、Kabayama, A.、Uchida, K、Hoshino, H.、Miwa, M.：“人类 T 细胞白血病病毒 1 型无细胞进入小鼠细胞。”Jpn.J.Cancer Res。

Uchida, M., et al.: "Overexpression of poly(ADP-ribose) polymerase disrupts organization of cytoskeletal F-actin and tissue polarity in Drosophila"J. Biol. Chem.. 277. 6696-6702 (2002)

Uchida, M., et al.：“聚（ADP-核糖）聚合酶的过度表达会破坏果蝇细胞骨架 F-肌动蛋白的组织和组织极性”J.

Miwa, M., Kanai, M., Sun, B., Hirai, M., Xm, X., Sitoh, S., Hanai, S., Kaneko, M., Nakagawara, A., Uchida, K.: "DNA methylation in neuroblastoma, DNA Methylation and Cancer, (S.Hirohashi, et al., ed.)"Princess Takamatsu Cancer Research Fund, Tokyo. (2001)

Miwa, M.、Kanai, M.、Sun, B.、Hirai, M.、Xm, X.、Sitoh, S.、Hanai, S.、Kaneko, M.、Nakakawara, A.、Uchida, K.：

内田 和彦: "SNPと疾患ゲノミクス"Clinical Neuroscience. 19. 104-105 (2001)

内田和彦：“SNP 和疾病基因组学”《临床神经科学》19. 104-105 (2001)。

内田 和彦: "DNAマイクロアレイと遺伝子発現情報データベース"化学と生物. 38. 676-683 (2000)

Kazuhiko Uchida：“DNA 微阵列和基因表达信息数据库”化学与生物学 38. 676-683 (2000)。