Development of high sensitive electrochemical DNA microarray

开发一种新的DNA芯片，克服现有DNA微阵列存在的问题，具有十分重要的意义。在本项目中，我们的目标是开发一种微电极，将合成的与Vanous遗传病相关的寡核苷酸固定在一起，并建立一种简单快速的检测患者基因组DNA杂交的电化学检测系统。一旦实现，这个系统将使我们能够有效地诊断癌症和传染病。1)微电极阵列制备技术的研究；2)电极上寡核苷酸探针的研究；3)电化学数据分析技术的研究；4)电化学阵列基因数据库的建立；5)血液样品制备方法的研究。我们还分析了胆管癌和甲状腺癌的基因组变化，以确定有价值的基因，以基因组诊断使用电化学阵列。所获得的知识有助于开发可用于分析遗传信息的便携式基因诊断仪。

It is very important to develop a new DNA chip, which overcomes the problem in the present DNA microarray. In this project, we aim at developing a microelectrode, which immobilizes a synthetic oligonucleotide concerned with vanous genetic diseases and establishing a simple and quick electrochemical detection system for the hybrid with genomic DNA taken from patients. Once realized, this system will allow us to diagnose cancers and infectious diseases efficiently. We have investigated the followings: 1) Study of the preparation technology of microelectrode arrays, 2) Study of the probes of oligonucleotide on the electrode, 3) Study of a technology to analyze electrocheinical data, 4) Establishment of cDNA database for electrochemical array, 5) Study of preparation method of blood samples. We also analyzed genomic alterations in the bile duct cancers and thyroid cancers to identify the valuable genes for genomic diagnosis using electrochernical array. The knowledge thus obtained is useful to develop a potable gene diagnosis instrument to analyze genetic information.