Characterization of a novel aspartic proteinase and development of its inhibitor

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We cloned a renin-like aspartic proteinase GS4001, which is identified mouse napsin, from mouse proximal tubule.The mouse napsin cleaved angiotensinogen and generated angiotensin I, while the human counterpart napsin did not cleave the angiotensinogen.The mouse napsin has a character of matrix metalloproteinase, and cleaved type IV collagen but not type I collagen.Napsin expression was declined in IgA nephripathy, minimal change nephritic syndrome, crescentic glomerulonephritis. The napsin expression was decreased in mouse BSA-overload model and unilateral ureteral obstruction model.We attempted to generate a new drug, which activates napsin activity to resolve the interstitial fibrosis.