Identification and characterization of stem cells for kidney

1)找到了骨髓来源细胞分化为肾小球系膜细胞的生物学条件。2)从肾脏中分离纯化SP细胞，并对其进行鉴定。3)我们证实了胎鼠微嵌合体在母鼠体内植入了胎儿细胞，这些细胞参与了慢性损伤后肝、肾的修复。据报道，孕妇携带的胎儿细胞是通过胎盘输入的。这些胎儿细胞在有妊娠史的女性体内存活并长期存在。这种现象被称为胎儿微嵌合体。在人类中，有一例存在长达27年的微嵌合体。据报道，在慢性甲状腺炎和丙型肝炎等慢性炎症病变中检测到胎儿细胞，由于胎儿细胞与母亲是半同种异体，它们可能会导致干燥综合征和硬皮病等自身免疫性疾病。我们研究了母鼠胎细胞与肾脏疾病的关系。在庆大霉素诱导的大鼠肾病模型中，我们发现胎儿细胞被整合到肾小管上皮结构中。这一结果表明，人类胎儿细胞也对母肾有贡献。4)建立了一种鉴定肾小球上皮细胞修复相关分子的方法。5)我们发现维甲酸在修复过程中起到了相关的作用，维甲酸促进了neparin的转录。

1)We found the biological condition under which bone marrow-derived cells differentiate into glomerular mesangial cells. 2)We purified SP cells from kidney, and characterized them. 3)We demonstrated that fetal cells were engragted in mother rats as a result of fetal microchimerism, and that the cells contributed to the repair of liver and kidney after chronic injury. Reportedly, pregnant women carry fetal cells which are imported through placenta. These fetal cells survive and exist for a long time in women with the history of pregnancy. This phenomenon is called fetal microchimerism. In human, there is a case with 27-year longstanding microchimerism. It is reported that fetal cells are detected in chronic inflammation lesions such as chronic thyroiditis and hepatitis C. Because fetal cells are semi-allogenic to mothers, they might cause autoimmune diseases such as Sjogren syndrome and scleroderma. We studied the relation between fetal cells in mother rats and renal diseases. In rat gentamcin-induced nephropathy, we found fetal cells were incorporated into tubular epithelial structure. This result suggests that human fetal cells also contribute to mother kidney. 4)We established a method to identify molecules which are involved in the repair of glomerular epithelial cells. 5)We found that retinoic acid plays a relevant role in the repair and that retinoic acid facilitates transcription of nephrin.