Preparation of non-biofouling blood bags

非生物污染血袋的制备

• 批准号: 12558103
• 负责人: IWASAKI Yasuhiko
• 金额: $ 7.3万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12558103/
• 关键词: Blood Bag; Polyorefine; Phosoholipid polymer; Blood compatibility; Protein adsorption; Platelet adhension; Photo-induced polymerization; Surface modification; 光重合; MPC; ポリエチレンフイルム; 生体適合性; SEM; 血小板粘着抑制効果

The surface of polyethylene (PE) was modified by grafting with various water-soluble polymers to control blood/materials interactions. This study was carried out in order to improve our understanding of the effect of chemical structure of water-soluble polymers grafted on the PE surface on platelet function when the platelets were in contacted the surface, Water-soluble polymers, such as poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC), poly(acrylamide) (PAAm), poly(N-vinylpyrrolidone) (PVPy), and poly[monomethacryloyl poly(ethylene glycol)] (PMPEG) were grafted on low-density PE sheets by photoinduced graft polymerization. PE bags modified with water-soluble polymers and non-modified PE bags were prepared by heat processing.The activation of platelets after storage in the PE bags was evaluated by measuring the cytoplasmic free calcium ion concentration ([Ca^<2+>]i). The [Ca^<2+>]i of platelets in contact with the PE surface grafted with PMPC was the same level as that of native platelets and significantly less than that in contact with other PE surfaces grafted with water-soluble polymers. The number of adherent platelets was effectively decreased on PE surfaces grafted with PMPC and PMPEG compared with non-treated PE. The aggregation ability of platelets was also measured after storage of platelet-rich plasma in the PE bags. The PE surface grafted with PMPC demonstrated a significantly decrease in aggregation ability compared with the non-treated PE, and that grafted with PAAm, PVPy, and PMPEG. We conclude that surface modification with PMPC is the most effective among these water-soluble polymers in preserving platelet functions.

通过接枝各种水溶性聚合物对聚乙烯（PE）表面进行修饰，以控制血液与材料的相互作用。本研究的目的是为了提高我们对PE表面接枝水溶性聚合物的化学结构对血小板功能的影响的认识，当血小板与PE表面接触时，水溶性聚合物如聚（2-甲基丙烯酰氧乙基磷胆碱）（PMPC）、聚（丙烯酰胺）（PAAm）、聚（n -乙烯基吡罗烷酮）（PVPy）、采用光诱导接枝聚合的方法将聚[单甲基丙烯酰聚乙二醇]（PMPEG）接枝到低密度PE片上。采用热处理法制备了水溶性高分子改性聚乙烯袋和非改性聚乙烯袋。通过测定细胞质游离钙离子浓度（[Ca^<2+>]i）来评估血小板在PE袋中储存后的活化情况。与PMPC接枝的PE表面接触的血小板的[Ca^<2+>]i与天然血小板相同，明显低于与其他水溶性聚合物接枝的PE表面接触的血小板。与未处理的PE相比，移植了PMPC和PMPEG的PE表面的粘附血小板数量有效减少。富血小板血浆储存于PE袋后，测定血小板聚集能力。与未处理的PE、PAAm、PVPy和PMPEG接枝的PE相比，PMPC接枝的PE表面的聚集能力明显降低。我们得出结论，在这些水溶性聚合物中，PMPC的表面改性在保护血小板功能方面是最有效的。

项目成果

Y.Iwasaki, N.Nakabayashi, and K.Ishihara: "Preservation of platelet function on 2-methacryloyloxyethyl phosphorylcholine graft polymer compared to various water-soluble graft polymers"J. Biomed. Mater. Res.. 57. 72-78 (2001)

Y.Iwasaki、N.Nakabayashi 和 K.Ishihara：“与各种水溶性接枝聚合物相比，2-甲基丙烯酰氧基乙基磷酰胆碱接枝聚合物保留血小板功能”J.

Y.Iwasaki, Y.Aiba, N.Nakabayashi, K.Ishihara: "Semi-interpenetrating polymer networks composed of biocompatible phcspholipid polymer and segmented polyurethane"Journal of Biomedical Materials Reseach. 51. 701-708 (2000)

Y.Iwasaki、Y.Aiba、N.Nakabayashi、K.Ishihara：“由生物相容性磷脂聚合物和嵌段聚氨酯组成的半互穿聚合物网络”生物医学材料研究杂志。

Yoneyama T,Ito M,Sugihara K,Ishihara K,Nakabayashi N: "Small Diameter Vascular Prosthesis with a Nonthrombogenic Phospholipid Polymer Surface : Preliminary Study of a new Concept for Functioning in the Absence of Pseudo-or Neointima Formation."Artifical O

Yoneyama T、Ito M、Sugihara K、Ishihara K、Nakabayashi N：“具有非血栓形成磷脂聚合物表面的小直径血管假体：在没有假性或新内膜形成的情况下发挥作用的新概念的初步研究。”人工 O

Iwasaki Y,Nakabayashi N,Ishihara K.: "Synthesis of novel phospholipid polymers by polycondensation."Macromol.Rapid Commun.. 21・6. 287-290 (2000)

Iwasaki Y、Nakabayashi N、Ishihara K.：“新型磷脂聚合物的缩聚合成”。Macromol.Rapid Commun. 21・6 (2000)。

Y.Iwasaki, Y.Aiba, N.Morimoto, N.Nakabayashi, and K.Ishihara,: "Semi-interpenetrating polymer networks composed of biocompatible phospholipid polymer and segmented polyurethane"J. Biomed. Mater. Res.. 51. 701-708 (2000)

Y.Iwasaki、Y.Aiba、N.Morimoto、N.Nakabayashi 和 K.Ishihara，“由生物相容性磷脂聚合物和分段聚氨酯组成的半互穿聚合物网络”J。