Pre-clinical study of gene therapy against refractory diseases in animals.
动物难治性疾病基因治疗的临床前研究。
基本信息
- 批准号:13460140
- 负责人:
- 金额:$ 11.2万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2001
- 资助国家:日本
- 起止时间:2001 至 2002
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The object of this study is the development of gene therapy against tumors and immune-abnormalities in veterinary field. In this research period, the study of 1) basic research of gene therapy by hepatocyte growth factor (HGF), 2) construction of adenovirus vector containing canine p53 and its effects for canine tumor cell lines, 3) gene therapy for and by feline immunodeficiency virus (FIV), have been conducted.1) HGF-gene therapy for liver and renal insufficiency in dogs and cats : Feline and canine HGF cDNA were cloned and their expressions were examined in various liver diseases in dogs together with tumor transforming growth factor (TGF). Furthermore, feline and canine HGF cDNA was inserted into expression plasmid and their efficient gene delivery method was examined.2) Gene therapy for tumors in dogs and cats : Aberrations of the p53 tumor suppressor gene in various tumors in dogs was investigated together with the abnormalities of centrosome. We next constructed adenovirus vector expressing canine p53 which was cloned in our laboratory and examined its cytocidal effects on canine tumor cell lines. Furthermore, feline IL-18 cDNA was cloned and expression plasmid for canine CTLA-4 fusion protein to enhance anti-tumor immunity.3) Gene therapy for FIV infection : We developed the novel method to quantify FIV RNA in plasma and an association of plasma viral RNA load with prognosis in cats naturally infected with FIV was investigated. We also investigated the mechanism of apoptosis of FIV-infected cells and cloned feline TNF receptors. Furthermore, feline vascular endotherial growth factor (VEGF) cDNA was cloned to develop new gene therapy against FIV infection.
本研究的目的是为肿瘤和免疫异常的基因治疗在兽医领域的发展做准备。在本研究期间,进行了以下研究:1)肝细胞生长因子(HGF)基因治疗的基础研究,2)含犬p53的腺病毒载体的构建及其对犬肿瘤细胞系的作用,3)猫免疫缺陷病毒(FIV)的基因治疗。1)犬和猫肝和肾功能不全的HGF基因治疗:克隆了猫和狗的肝细胞生长因子cDNA,并检测了它们在狗的各种肝病中的表达以及肿瘤转化生长因子(TGF)。2)犬、猫肿瘤的基因治疗:研究犬各种肿瘤中p53抑癌基因的异常和中心体的异常。我们构建了表达犬p53的腺病毒载体,并检测了其对犬肿瘤细胞系的杀伤作用。进一步克隆了猫IL-18 cDNA,构建了犬CTLA-4融合蛋白的表达质粒,以增强抗肿瘤免疫力。3)FIV感染的基因治疗:建立了FIV感染猫血浆中FIV RNA定量的新方法,并研究了血浆病毒RNA载量与FIV感染猫预后的关系。我们还研究了FIV感染细胞的凋亡机制和克隆的猫TNF受体。本研究还克隆了猫血管内皮生长因子(VEGF)cDNA,为开发新的抗FIV感染的基因治疗方法奠定了基础。
项目成果
期刊论文数量(42)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Effect of an adenoviral vector that expresses the canine p53 gene on cell growth of canine osteosarcoma and mammary adenocarcinoma cell lines.
表达犬p53基因的腺病毒载体对犬骨肉瘤和乳腺癌细胞系细胞生长的影响。
- DOI:
- 发表时间:2003
- 期刊:
- 影响因子:0
- 作者:Yazawa;M.;et al.
- 通讯作者:et al.
Kanaya, N.: "Detection of the anti-P53 antibodies in a dog with tumor"J. Vet. Med. Sci.. 64. 973-979 (2002)
Kanaya,N.:“检测患有肿瘤的狗中的抗 P53 抗体”J。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Aberrations of the p53 tumor suppressor gene in various tumors in dogs
- DOI:10.2460/ajvr.2001.62.433
- 发表时间:2001-03-01
- 期刊:
- 影响因子:1
- 作者:Setoguchi, A;Sakai, T;Tsujimoto, H
- 通讯作者:Tsujimoto, H
Telomere length and telomerase activity in canine mammary gland tumors.
犬乳腺肿瘤中的端粒长度和端粒酶活性。
- DOI:
- 发表时间:2001
- 期刊:
- 影响因子:0
- 作者:Yazawa;M.;et al.
- 通讯作者:et al.
Molecular cloning of feline hepatocyte growth factor (HGF)cDNA.
猫肝细胞生长因子 (HGF)cDNA 的分子克隆。
- DOI:
- 发表时间:2001
- 期刊:
- 影响因子:0
- 作者:Kobayashi;Y.;et al.
- 通讯作者:et al.
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OHNO Koichi其他文献
Effects of budesonide administration on coagulation variables in dogs.
布地奈德给药对狗凝血变量的影响。
- DOI:
- 发表时间:
2019 - 期刊:
- 影响因子:0
- 作者:
NAGAHARA Takuro;OHNO Koichi;NAGAO Itsuma;NAKAGAWA Taisuke;YOKOYAMA Nozomu;OHMI Aki;GOTO-KOSHINO Yuko;CHAMBERS James K.;UCHIDA Kazuyuki;TOMIYASU Hirotaka;TSUJIMOTO Hajime;永原 拓朗;Takuro Nagahara - 通讯作者:
Takuro Nagahara
無発話の自閉スペクトラム症児に対する日常環境発達行動支援 ―オンライン型保護者トレーニング介入の効果―
自闭症谱系障碍非语言儿童的日常环境发展行为支持-在线家长培训干预的效果-
- DOI:
- 发表时间:
2023 - 期刊:
- 影响因子:0
- 作者:
NAGAHARA Takuro;OHNO Koichi;NAGAO Itsuma;NAKAGAWA Taisuke;YOKOYAMA Nozomu;OHMI Aki;GOTO-KOSHINO Yuko;CHAMBERS James K.;UCHIDA Kazuyuki;TOMIYASU Hirotaka;TSUJIMOTO Hajime;永原 拓朗;Takuro Nagahara;韓天一・山本淳一 - 通讯作者:
韓天一・山本淳一
蛋白喪失性腸症の犬における治療に伴う凝固系パラメーターの変化
治疗引起的蛋白质丢失性肠病犬凝血系统参数的变化
- DOI:
- 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
NAGAHARA Takuro;OHNO Koichi;NAGAO Itsuma;NAKAGAWA Taisuke;YOKOYAMA Nozomu;OHMI Aki;GOTO-KOSHINO Yuko;CHAMBERS James K.;UCHIDA Kazuyuki;TOMIYASU Hirotaka;TSUJIMOTO Hajime;永原 拓朗 - 通讯作者:
永原 拓朗
自閉スペクトラム症児の模倣を促進する試行間間隔要因の分析
促进自闭症谱系障碍儿童模仿的间歇期因素分析
- DOI:
- 发表时间:
2022 - 期刊:
- 影响因子:0
- 作者:
NAGAHARA Takuro;OHNO Koichi;NAGAO Itsuma;NAKAGAWA Taisuke;YOKOYAMA Nozomu;OHMI Aki;GOTO-KOSHINO Yuko;CHAMBERS James K.;UCHIDA Kazuyuki;TOMIYASU Hirotaka;TSUJIMOTO Hajime;永原 拓朗;Takuro Nagahara;韓天一・山本淳一;韓天一・山本淳一 - 通讯作者:
韓天一・山本淳一
OHNO Koichi的其他文献
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{{ truncateString('OHNO Koichi', 18)}}的其他基金
Classification of drinking water quality standard items based on uncertainty analysis in risk assessment
基于风险评估不确定性分析的饮用水水质标准项目分类
- 批准号:
24510052 - 财政年份:2012
- 资助金额:
$ 11.2万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Automated Exploration of Structures of Molecular Aggregates based on Novel Algorithms for Global Optimization
基于全局优化新算法的分子聚集体结构的自动探索
- 批准号:
23655021 - 财政年份:2011
- 资助金额:
$ 11.2万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Study on water purification method by multiple adsorbents for effective removal of broad-spectrum organic pollutants
有效去除广谱有机污染物的多种吸附剂净水方法研究
- 批准号:
22760403 - 财政年份:2010
- 资助金额:
$ 11.2万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Development of a High-Speed Automated Exploration Method for Reaction Routes and Application to Selective Reaction Control
反应路线高速自动探索方法的开发及其在选择性反应控制中的应用
- 批准号:
21350007 - 财政年份:2009
- 资助金额:
$ 11.2万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Establishment of novel non-invasive test for differential diagnosis and severity evaluation of hepatobiliary diseases in dogs and cats.
建立新型非侵入性检测方法,用于犬和猫肝胆疾病的鉴别诊断和严重程度评估。
- 批准号:
21380193 - 财政年份:2009
- 资助金额:
$ 11.2万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Estimation and improvement of arsenic intake of a population living in arsenic-affected area in Bangladesh
孟加拉国砷污染地区人口砷摄入量的估算和改进
- 批准号:
20760354 - 财政年份:2008
- 资助金额:
$ 11.2万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Development of cross-correlation highly sensitive electron spectroscopy and real-time measurement of chemical reactions at interface
互相关高灵敏电子能谱研制及界面化学反应实时测量
- 批准号:
17205001 - 财政年份:2005
- 资助金额:
$ 11.2万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Clinical application of the molecular target therapy for natural occurring tumors in small animals.
分子靶向治疗小动物天然肿瘤的临床应用。
- 批准号:
15380213 - 财政年份:2003
- 资助金额:
$ 11.2万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Orientation and dynamics of molecules on surface observed by highly sensitive Penning ionization electron spectroscopy
高灵敏潘宁电离电子能谱观察表面分子的取向和动力学
- 批准号:
14340173 - 财政年份:2002
- 资助金额:
$ 11.2万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of Time and Space Resolved 2-Dimensional Penning Ionization Electron Spectroscopy
时空分辨二维潘宁电离电子能谱的发展
- 批准号:
07554024 - 财政年份:1995
- 资助金额:
$ 11.2万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
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