Transplantation of hepatocyte-like cells differentiated from ES cells

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1)A study of induction of hepatocytefrom ES cellWe have succeeded in inducing albumin-producing cell through EB from ES cell. These cell had a ability of producing urea. Taken these together, these cells are considered to be hepatocyte. This hepatocyte-like cell was transplanted into C57BL/6 mouse which had liver injury in advance. The transplanted cell grew up in the recipient liver and produced albumin. But, teratoma formation was seen in 20-30% mouse. The cell was selected in order to suppress teratoma. First, the single layer cell culture from EB was performed, and isolated. The cell was selected by specific gravity by the ercoll method. This cell group was negative in oct 3/4. When this cell was transplanted to an above-mentioned liver injury model mouse, teratoma formation was not recognized. As mentioned above, recovery of the cell group which does not generate teratoma from an embryonic stem cell was attained. Furthermore, it became possible by removing other blood cell ingredients for this cell group using FAGS and MACS to obtain many albumin positive cells.2) ES cell of cynomolgus monkeyAiming at clinical application of embryonic stem cell, induction of a liver cell was tried from cynomolgus monkey embryonic stem cell. Although a different method of induction from a mouse embryonic stem cell was required for induction of cynomolgus monkey stem cell, albumin-producing cell was able to be induced by our method.3) Induction of hepatocyte from umbilical cord bloodWe succeeded in liver cell induction from umbilical cord blood. Two cell types were recognized, one is expressing albumin and CK-18 and the other is expressing albumin and CK-19. Now, we are studying bile duct epithelium cell induction from umbilical cord blood.