APPLICATION OF NATURAL ORGANIC BIOACTIVE MATERIAL, CHITOSAN TO PREVENTION AND CURE FOR DENTAL CARIES

天然有机生物活性物质壳聚糖在龋齿防治中的应用

• 批准号: 13470404
• 负责人: HAYASHI Yoshihiko
• 金额: $ 6.53万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470404/
• 关键词: Chitosan; Microgravity; Osteoblasts; Alkaline phosnhatase; 水溶性キトサン; キトサンオリゴ糖; 生物学的石灰化; 齲蝕予防; 齲蝕治療; バイオマテリアル

A clinostat is. a microgravity stimulator that can rotate the object on a plate perpendicularly to axis of gravity and cancel out loads from certain direction and to confuse the gravisensors of organisms although clinostat itself can not stimulate microgravity. It is shown that stimulated microgravity conditions obtained in the clinostat depress proliferation and differentiation in osteoblastic cells. In this study, we investigated the effects of water-soluble chitosan to osteoblasts whose function was suppressed by clinostat.NOS-1 cells were round or polygonal shape with monolayer in each group. All groups were shown the cluster of cells, but the cluster of clinorotation and chitosan groups were smaller than other two groups. Proliferation of cells in chitosan group was promoted as compared with one of clinorotation group. After 3 days of incubation, alkaline phosphatase (ALP) activities of each group were evaluated. The chitosan group showed significantly higher activity than other three groups, whereas ALP activity of the clinorotation group was lower than them (p<0.05). The present findings suggest that water-soluble chitosan could act as the accelerator for the proliferation and differentiation of osteoblastic cells under the artificial hypofunctional condition using clinostat.

恒温器是。一种微重力刺激器，它可以使平板上的物体垂直于重力轴旋转，并抵消来自某个方向的载荷，从而混淆生物体的重力传感器，但它本身不能刺激微重力。结果表明，在恒温器中获得的刺激微重力条件会抑制成骨细胞的增殖和分化。在本研究中，我们研究了水溶性壳聚糖对缓凝剂抑制成骨细胞的作用。NOS-1细胞呈圆形或多角形，呈单层结构。各组均可见细胞簇，但壳聚糖组细胞簇小于其他两组。壳聚糖组细胞的增殖能力明显高于壳聚糖组。孵育3 d后，测定各组碱性磷酸酶（ALP）活性。壳聚糖组ALP活性显著高于其他3组（p<0.05），而陈化组ALP活性显著低于其他3组（p<0.05）。本研究结果提示，水溶性壳聚糖可作为成骨细胞增殖和分化的促进剂。

项目成果

Ohra N, Hayashi Y, Yamada S, Kim S-K, Matsunaga T, Yanagiguchi K, Ikeda T: "Early gene expression analyzed by cDNA microarray and RT-PCR in osteoblasts cultured with water-soluble and low molecular chitooligosaccharide"Biomaterials. 25・10. 1749-1754 (2004

Ohra N、Hayashi Y、Yamada S、Kim S-K、Matsunaga T、Yanagiguchi K、Ikeda T：“通过 cDNA 微阵列和 RT-PCR 分析水溶性低分子壳寡糖培养的成骨细胞中的早期基因表达”25・10。 .1749-1754 (2004

Yamaguchi K, Toda K, Hayashi Y: "Stressful training changes endogenous neurotransmitters in human plasma"Stress and Health. (in press). (2004)

Yamaguchi K、Toda K、Hayashi Y：“压力训练会改变人血浆中的内源性神经递质”压力与健康。

Fujiwara M, Hayashi Y, Ohara N: "Inhibitory effect of water-soluble chitosan on growth of Streptococcus mutans"Microbiologica. 27・1. 83-86 (2004)

藤原M、林Y、小原N：“水溶性壳聚糖对变形链球菌生长的抑制作用”微生物学27・1（2004）。

Ohara N, Hayashi Y, Yamada S, Kim S-K, Matsunaga T, Yanagiguchi K, Ikeda T: "Early gene expression analyzed by cDNA microarray and RT-PCR in osteoblasts cultured with water-soluble and low molecular chitooligosaccharide"Biomaterials. 25・10. 1749-1754 (200

Ohara N、Hayashi Y、Yamada S、Kim S-K、Matsunaga T、Yanagiguchi K、Ikeda T：“通过 cDNA 微阵列和 RT-PCR 分析水溶性低分子壳寡糖培养的成骨细胞中的早期基因表达”25・10。 1749-1754（200）

林 善彦, 柳口嘉治郎, 大原直子, 金 世権: "キトサンオリゴ糖の生物学的石灰化促進機構に関する韓国釜慶大学校との共同研究"平成13年度長崎大学教育研究改革・改善プロジェクト研究成果報告書. 83-88 (2002)

Yoshihiko Hayashi、Kajiro Yanagguchi、Naoko Ohara、Se-geon Kim：“与韩国富庆大学联合研究壳寡糖的生物矿化促进机制”2001年长崎大学教育研究改革和改进项目研究成果报告83-88（。 2002）