Development of regenerative medicine using human iPS cellsFor pulp and periapical pathoses

使用人类 iPS 细胞开发再生医学治疗牙髓和根尖周病

• 批准号: 22390360
• 负责人: HAYASHI Yoshihiko
• 金额: $ 12.23万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390360/
• 关键词: 再生医療; iPS細胞; 低酸素培養; 細胞増殖・分化; 骨芽細胞誘導

There are no stastical differences in either the growth and differentiation of (induced pluripotent stem) iPS cells or the induction of iPS cells to osteoblasts between the four and three transcription factor froups cultured under hypoxia. Furthermore, the function of (hypoxia inducible factors) HIFs in murine iPS cells under hypoxia was investigated in relation to the morphology and expression of the transcription factors. The HIF-2α knockdown group exhibited a decreased colony size in the iPS cells. The HIF-2α or -3α demonstrated a statistical significant decrease in the expression of the transcription factors compared to those observed in the control group. These results demonstrate that HIF-2α among HIFs is the most influential candidate for the maintenance of the pluripotency of murine iPS cells.

在低氧条件下培养的诱导多能干细胞的生长和分化以及诱导iPS细胞向成骨细胞的分化方面，四种转录因子组与三种转录因子组之间没有统计学差异。此外，（缺氧诱导因子）HIF在小鼠iPS细胞在缺氧条件下的功能进行了研究与形态和转录因子的表达。HIF-2α敲低组iPS细胞集落大小减少。与对照组相比，HIF-2α或-3α组的转录因子表达出现统计学显著性降低。这些结果表明，HIF-2α是HIF中维持小鼠iPS细胞多能性的最有影响力的候选者。

项目成果

Marine Cosmeceuticals: Trends and Prospects

海洋药妆：趋势与前景

• 发表时间: 2012
• 作者: 多田浩之;島内英俊;松下健二;川島伸之;Hayashi Y
• 通讯作者: Hayashi Y

Marine Medical Food, Volume 65, Advances in Food and Nutrition Research

海洋医疗食品，第 65 卷，食品和营养研究进展

• 发表时间: 2012
• 作者: Hayashi Y;Yamada S;Yanagiguchi K;Koyama Z;Ikeda T(分担)
• 通讯作者: Ikeda T(分担)

Effects of glucosamine on the tooth pulpal nociceptive responses in the rat

氨基葡萄糖对大鼠牙髓伤害性反应的影响

• 发表时间: 2013
• 期刊: Journal of Dental Sciences
• 影响因子: 3.5
• 作者: Kaida K;Yamashita H;Toda K;Hayashi Y.
• 通讯作者: Hayashi Y.

低酸素下におけるiPS細胞の増殖・分化挙動

缺氧条件下iPS细胞的增殖和分化行为

• 发表时间: 2011
• 作者: 杉本浩司;石崎秀隆;池田毅;林善彦
• 通讯作者: 林善彦

Effects of eluted components from 4-META/MMA-TBB adhesive resin sealer on osteoblastic cell proliferation

• DOI: 10.1016/j.jds.2012.03.002
• 发表时间: 2012-06-01
• 期刊: JOURNAL OF DENTAL SCIENCES
• 影响因子: 3.5
• 作者: Kawasaki, Aya;Hayashi, Yoshihiko;Fujiwara, Mamoru
• 通讯作者: Fujiwara, Mamoru