Degradation of xenobiotic compounds and dehalogenation by using multi enzyme systems of a newly isolated fungus, Geotrichum canididum Dec 1
使用新分离的真菌(Geotrichum canididum)的多酶系统降解外源化合物和脱卤 12 月 1 日
基本信息
- 批准号:13555223
- 负责人:
- 金额:$ 8.58万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2001
- 资助国家:日本
- 起止时间:2001 至 2002
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
(1) The effluent from kraft pulp bleaching process was decolorized by G. canididum Dec 1 and the color removal ratio reached 70-80%. The decolorization process by Dec 1 consists of two steps, namely the first adsorption of color substances to the surface of the cells and the second biodegradation by the cells. Simultaneously, the absorbable organic halogens (AOX) that correspond to the aromatic compounds containing halogen was reduced by 43%, indicating that Dec 1 conducted dehalogenation in parallel to decolorization of lignin in the effluent.(2) The effluent from oxygen-delignified process was decolorized by Dec 1 and the color removal ratio reached 90%. Bioleaching of oxygen-delignified pulp was carried out by the culture broth of Dec 1 and the brightness of the pulp increased 30 points and the kappa number decreased by 10 points. After the effluent was decolorized by Dec 1, the effluent was mixed with the pulp and the bleaching was continued. As the brightness of the pulp increased … More significantly, Dec 1 showed potential of consecutive treatment of the effluent and the bleaching of the pulp.(3) Bleaching of unbleached kraft pulp was tried by using the culture of Dec 1. The increase in brightness and the reduction of the kappa number were almost equivalent to or more than those of well-studied basidiomycetes. After the unbleached kraft was bleached by Dec 1, the pulp was washed and mixed with the freshly grown Dec 1 culture and the second bleaching was continued. The increase in brightness reached 50 points and this values demonstrate the usefulness of the bio-bleaching of the pulp by Dec 1.(4) Peroxidase (DyP) and manganese peroxidase (MnP) were purified from the culture broth of Dec 1. As the activities of DyP an MnP were detected during decolorization of oxygen-delignified effluent, bleaching of oxygen-delignified pulp and bleaching of unbleached kraft pulp, the two enzymes are found to be heavily involved in those reactions. The higher activity of MnP in the degradation of lignin was newly found.(5) Lignin-degradation ability of Dec 1 was confirmed by the plate test and by the liquid culture using commercial lignin. The color of lignin changed from brown to yellow in plate test. It was confirmed by gel chromatography that the average molecular mass of the original lignin, 80,000 was reduced to less than 3,000. As the activities of DyP and MnP were detected, this is the first report that the existence of two enzymes involved in lignin degradation was found other than basidiomycetes. The degradation of the dye, Poly R-478 by the two enzymes was also clarified. Less
(1)对硫酸盐纸浆漂白出水进行了木浆菌(G. canidim . Dec 1)脱色,脱色率达70 ~ 80%。到12月1日的脱色过程包括两个步骤,即第一步将颜色物质吸附到细胞表面,第二步由细胞进行生物降解。同时,与含卤素芳香族化合物对应的可吸收有机卤素(AOX)降低了43%,表明Dec 1在对出水木质素进行脱色的同时进行了脱卤处理。(2)用Dec - 1对氧脱木质素工艺出水进行脱色,脱色率达90%。用12月1日的培养液对氧脱木质素纸浆进行生物浸出,纸浆的亮度提高了30点,kappa值降低了10点。12月1日出水脱色后,将出水与纸浆混合,继续漂白。随着纸浆亮度的提高,12月1日显示出连续处理废水和纸浆漂白的潜力。(3)利用12月1日培养物对未漂白硫酸盐浆进行了漂白试验。其亮度的增加和kappa数的降低几乎等于或超过研究充分的担子菌。在12月1日之前,未漂白的牛皮纸被漂白后,将纸浆洗涤并与新鲜生长的12月1日培养物混合,继续进行第二次漂白。亮度的增加达到了50点,这个值表明了在12月1日之前对纸浆进行生物漂白的有效性。(4)从12月1日培养液中纯化出过氧化物酶(DyP)和锰过氧化物酶(MnP)。在氧脱木质素出水脱色、氧脱木质素纸浆漂白和未漂白硫酸盐纸浆漂白过程中检测了DyP和MnP的活性,发现这两种酶在这些反应中起重要作用。新发现MnP对木质素具有较高的降解活性。(5)通过平板试验和商用木质素液体培养证实了Dec - 1降解木质素的能力。平板试验木质素的颜色由棕色变为黄色。凝胶色谱法证实,原木质素的平均分子质量由80000降至3000以下。DyP和MnP的活性检测表明,这是除担子菌外,首次发现两种参与木质素降解的酶。并阐明了这两种酶对Poly R-478染料的降解作用。少
项目成果
期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Noboru Shintani, Yasushi Sugano and Makoto Shoda: "Decolorization of kraft pulp bleaching effluent by a newly isolated fungus, Geotrichum canididum Dec 1"J. Wood Science. 48. 402-408 (2002)
Noboru Shintani、Yasushi Sugano 和 Makoto Shoda:“新分离出的真菌(Geotrichum canididum)对牛皮纸浆漂白废水的脱色,12 月 1 日”J.
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
S.Kim, N.Suzuki, Y.Unemasu, M.Shoda: "Characterization of Aryl Alcohol Oxidase Produced by Decolorizing Fungus, Geotrichum candidum Dec 1"J. Biosci. Bioeng.. 91(2). 166-172 (2001)
S.Kim、N.Suzuki、Y.Unemasu、M.Shoda:“脱色真菌、白地霉 12 月 1 日产生的芳醇氧化酶的特性”J。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
N.Shintani, Y.Sugano, M.Shoda: "Decolorization of kraft pulp bleaching effluent by a newly and isolated fungus, Geotrichum candidum Dec 1"J.Wood Science. 48・5. 402-408 (2002)
N.Shintani、Y.Sugano、M.Shoda:“新分离的真菌白地霉对牛皮纸浆漂白废水的脱色”J.Wood Science 48・5 (2002)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
N.Shintani, Y.Sugano, M.Shoda: "Decolorization of kraft pulp bleaching effluent by a newly isolated fungus, Geotrichum candidum Dec 1"J. Wood Science. 48・5. 402-408 (2002)
N.Shintani、Y.Sugano、M.Shoda:“新分离的真菌白地霉的脱色”J. 48・5(2002 年)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Y.Sugano, C.Matsuo, M.Shoda: "Efficient Production of a Heterologous Peroxidase, DyP from Geotrichum candidum Dec 1, on Solid-State Culture of Aspergillus oryzae RDOO5"J. Biosci. Bioeng.. 92(6). 594-597 (2001)
Y.Sugano、C.Matsuo、M.Shoda:“在米曲霉 RDOO5 固态培养物上,从 12 月 1 日白地霉中高效生产异源过氧化物酶 DyP”J。
- DOI:
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- 影响因子:0
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SHODA Makoto其他文献
SHODA Makoto的其他文献
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{{ truncateString('SHODA Makoto', 18)}}的其他基金
Development of environmentally friendly and multifunctional microbial pesticides by using Bacillus subtilis
利用枯草芽孢杆菌开发环保多功能微生物农药
- 批准号:
11450312 - 财政年份:1999
- 资助金额:
$ 8.58万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Optimal system of bacterial cellulose production as an alternative of plant cellulose
作为植物纤维素替代品的细菌纤维素生产的最佳系统
- 批准号:
11558072 - 财政年份:1999
- 资助金额:
$ 8.58万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
CREATION OF MICROBIAL PESTICIDE BY BACILLUS SUBTILIS AND CONSTRUCTION OF RECYCLE SYSTEM
枯草芽孢杆菌创制微生物农药及回收系统的构建
- 批准号:
07555558 - 财政年份:1995
- 资助金额:
$ 8.58万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
BIOTECHNOLOGY OF TREATMENT OF VOLATILE ORGANIC COMPOUNDS (VOC)
处理挥发性有机化合物(VOC)的生物技术
- 批准号:
06454035 - 财政年份:1994
- 资助金额:
$ 8.58万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
相似海外基金
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VERY HIGH RESOLUTION OF MANGANESE PEROXIDASE FROM PHANEROCHAETE CHRYSOSPORIUM
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6586742 - 财政年份:2002
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MODELING MANGANESE PEROXIDASE--A REACTIVITY STUDY
锰过氧化酶建模——反应性研究
- 批准号:
6642040 - 财政年份:2001
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$ 8.58万 - 项目类别:
MODELING MANGANESE PEROXIDASE--A REACTIVITY STUDY
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6437660 - 财政年份:2001
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MODELING MANGANESE PEROXIDASE--A REACTIVITY STUDY
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- 批准号:
6525851 - 财政年份:2001
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$ 8.58万 - 项目类别:
Regulation of Manganese Peroxidase Gene Expression
锰过氧化物酶基因表达的调控
- 批准号:
9723725 - 财政年份:1997
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$ 8.58万 - 项目类别:
Continuing Grant
HIGH RESOLUTION STUDIES OF MANGANESE PEROXIDASE FROM PHANEROCHAETE CHRYSOSPORIUM
黄孢原毛毛革菌锰过氧化酶的高分辨率研究
- 批准号:
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Regulation of Manganese Peroxidase Gene Expression
锰过氧化物酶基因表达的调控
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$ 8.58万 - 项目类别:
Continuing grant