CREATION OF MICROBIAL PESTICIDE BY BACILLUS SUBTILIS AND CONSTRUCTION OF RECYCLE SYSTEM

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A massive organic wastes from food industry are produced under the situation that proper treatment methods have not been developed. On the other hand, excessive use of chemical pesticides caused environmental problems even in agricultural area. To solve the two problems simulatmenously, the research was conducted and the followings are the summary of the results.(1) Newly isolated bacteria, Bacillus subtilis NB22, RB14 and YB8, were cultivated in solid state fermentation, using soybean curd residur, Okara as a substrate to produce microbial pesticede. In scale-up from 15g to 3kg, optimal condition was investigated and temperature control and aeration were found to be crucial factors for the production of the antibiotics by the bacteria.(2) The oraka fermented product was treated to soil and the growth effect of the product on the plant was confirmed in a pot test.(3) The culture broth, cell suspension and cell-free broth of the bacteria were applied to soil infested with plant pathogen, Rhizoctonia solani of damping-off of tomato and the suppressive effect of the bacteria to the plant disease was proved. The concentrations of the cells, antibiotics in soil were determined and the role of the antibiotics to control the desease was confirmed.(4) The genes which are responsible for the production of the antibiotics were cloned, designated as lpa. The role of the genes for the suppression of the disease was also confirmed in plant test.(5) Basic systems analysis for construction of recycle system of the organic wastes produced in Kanagawa Prefecture was conducted by using simple chemical process was carried out.