Optimal system of bacterial cellulose production as an alternative of plant cellulose

作为植物纤维素替代品的细菌纤维素生产的最佳系统

基本信息

  • 批准号:
    11558072
  • 负责人:
  • 金额:
    $ 8.64万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
  • 财政年份:
    1999
  • 资助国家:
    日本
  • 起止时间:
    1999 至 2000
  • 项目状态:
    已结题

项目摘要

Bacterial cellulose (BC) was produced by a bacterium, Acetobacter xylinum BPR2001 and the following results were obtained.(A) Cultivation by an airlift reactor(1)BC productivity by the A.xylinum was enhanced when the oxygen-rich air was supplied into the airlift reactor and the productivity was comparable to that in a jar fermentor.When fructose as a carbon source was increased to 60-70g/l, the highest production rate of 0.22 g/l/h which was two-fold of the previous data and the yield of BC to sucrose of 35% were assessed. The BC obtained in an airlift reactor was formed to be unique pellets and the diameter of the pellets was smaller when the productivity of BC was higher. The carbon balance of the cultivation revealed that the production of carbon dioxide and other carbon containing byproducts was reduced when the productivity was higher, indicating that the energy efficiency was increased.(2) addition of water-soluble polysaccharides like agar or xanthan into the medium increased the productivity of BC, presumably due to increase in viscosity and this caused the clump formation of cells and BC.(B) Cultivation in a jar fermentorThe 10 liter jar fermentor was used to compare the production of BC with that in airlift reactor. The production of BC was reduced about 10% in a jar fermentor when 70g/l of fructose was used. The enhanced effect of BC production was not observed when water-soluble polysaccharides were added.(C) Comparison of airlift reactor and jar fermentorWhen fructose concentration was moderate, the productivity of BC between the two reactors was not different. However, when the fructose concentration was increased, the production of BC in an airlift reactor was significantly higher than that in a jar fermentor. The consumed energy in the airlift reactor was one-fifth to one-sixth of the jar fermentor.
细菌纤维素(BC)由细菌木醋杆菌(Acetobacter xylinum)BPR 2001产生,并获得以下结果。(A)气升式反应器培养(1)在气升式反应器中通入富氧空气可提高木曲霉的BC生产能力,其生产能力与罐式发酵罐相当,当碳源果糖增加到60- 70 g/l时,最高生产速率为0.22 g/l/h,是以前数据的两倍,BC对蔗糖的产率为35%。在气升式反应器中获得的BC形成独特的颗粒,并且当BC的产率较高时,颗粒的直径较小。培养的碳平衡显示,当生产率较高时,二氧化碳和其他含碳副产物的产生减少,表明能量效率提高。(2)向培养基中加入水溶性多糖如琼脂或黄原胶增加了BC的产率,这可能是由于粘度增加,这导致细胞和BC的团块形成。(B)罐式发酵罐培养使用10升罐式发酵罐来比较BC与气升式反应器的产量。在罐式发酵罐中,当果糖用量为70 g/l时,BC产量下降约10%。添加水溶性多糖对细菌纤维素产量的促进作用不明显。(C)气升式反应器与罐式发酵器的比较在果糖浓度适中的情况下,两种反应器的发酵产率没有差异。然而,当果糖浓度增加时,在气升式反应器中BC的产量显著高于在罐式发酵罐中。气升式反应器的能耗仅为罐式发酵罐的五分之一到六分之一。

项目成果

期刊论文数量(26)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Y-p.Chao,T.Sugano,M.Shoda: "Bacterial Cellulose Production by Acetobacter xyliruun in a 50 L Internal Loop Airlift Reactor"Biotechnol.Bioeng.印刷中.
Y-p.Chao、T.Sugano、M.Shoda:“在 50 L 内部循环气升式反应器中通过 Acetobacter xyliruun 生产细菌纤维素”Biotechnol.Bioeng。
  • DOI:
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  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Akito Tonoike, Fumihiro Yoshinaga: "Molecular breeding of bacterial cellulose high production bacterium"Enzyme Engineering News. 41. 11-15 (1999)
Akito Tonoike、Fumihiro Yoshinaga:“细菌纤维素高产细菌的分子育种”酶工程新闻。
  • DOI:
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    0
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  • 通讯作者:
Yaping Chao, Makoto Mitarai, Yasushi Sugano, and Makoto Shoda: "Effect of Addition of Water Soluble Polysaccharides on Bacterial Cellulose Production in a 50-L Airlift Reactor"Biotechnol.Progress. (in press). (2001)
Yaping Chao、Makoto Mitarai、Yasushi Sugano 和 Makoto Shoda:“添加水溶性多糖对 50 L 气升式反应器中细菌纤维素生产的影响”生物技术进展。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Y.Chao,T.Ishida,Y.Sugano and M.Shoda: "Bacterial Cellulose Production by Acetobacter xylinum in a 50-L Internal-Loop Airlift Reactor"Biotechnol.Bioeng.. 68. 345-352 (2000)
Y.Chao、T.Ishida、Y.Sugano 和 M.Shoda:“50 L 内部循环气升反应器中的木醋杆菌生产细菌纤维素”Biotechnol.Bioeng.. 68. 345-352 (2000)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Y.Chao,Y.Sugano,and M.Shoda: "Bacterial Cellulose Production under Oxygen-Enriched Air at Different Fructose Concentrations in a 50-L Internal Loop Airlift Reactor"Appl.Microbiol.Biotechnol.. (in press).
Y.Chao、Y.Sugano 和 M.Shoda:“50 升内循环气升式反应器中不同果糖浓度的富氧空气下的细菌纤维素生产”Appl.Microbiol.Biotechnol..(印刷中)。
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SHODA Makoto其他文献

SHODA Makoto的其他文献

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{{ truncateString('SHODA Makoto', 18)}}的其他基金

Degradation of xenobiotic compounds and dehalogenation by using multi enzyme systems of a newly isolated fungus, Geotrichum canididum Dec 1
使用新分离的真菌(Geotrichum canididum)的多酶系统降解外源化合物和脱卤 12 月 1 日
  • 批准号:
    13555223
  • 财政年份:
    2001
  • 资助金额:
    $ 8.64万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of environmentally friendly and multifunctional microbial pesticides by using Bacillus subtilis
利用枯草芽孢杆菌开发环保多功能微生物农药
  • 批准号:
    11450312
  • 财政年份:
    1999
  • 资助金额:
    $ 8.64万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
CREATION OF MICROBIAL PESTICIDE BY BACILLUS SUBTILIS AND CONSTRUCTION OF RECYCLE SYSTEM
枯草芽孢杆菌创制微生物农药及回收系统的构建
  • 批准号:
    07555558
  • 财政年份:
    1995
  • 资助金额:
    $ 8.64万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
BIOTECHNOLOGY OF TREATMENT OF VOLATILE ORGANIC COMPOUNDS (VOC)
处理挥发性有机化合物(VOC)的生物技术
  • 批准号:
    06454035
  • 财政年份:
    1994
  • 资助金额:
    $ 8.64万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

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