High Speed and Large Scale Submerged Culture of Useful Mushroom Mycelium in Bubble Column Bioreactors

有用蘑菇菌丝体在泡罩生物反应器中的高速大规模深层培养

基本信息

批准号：
13555229
负责人：
KAWAGOE Mikio
金额：
$ 4.86万
依托单位：
Nara National College of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

(1) Submerged culture of useful mushroom mycelium: We found new type of Tricoloma matsutake mycelium of which growth rate was five time as high as that of original species in our laboratory. This new type of mycelium was cultured in a standard bubble column to find the optimum conditions on medium composition, pH, and aeration rate (Kawagoe and Itoh ; Research Reports of Nara National College of Technology, 38, 83-86 (2002)). Also, we estimated apparent viscosity of suspended medium of T. matsutake mycelium from the circulation velocity in an external-loop airlift column (Kawagoe ; The Japanese society for multiphase flow, Annual Meeting 2002, B118 (2002)). Optimum conditions on aeration rate, pH, and medium compositions were also found for submerged culture of Agaricus blazei in a standard bubble column bioreactor (Kawagoe. ; unpublished).(2) Measurement of mycelium pellets concentration by laser transmission method : We developed a novel measurement method of mycelium pellets concent … More ration by laser transmission. From the laser transmission and pulse width, concentration and size of mycelium pellets were estimated simultaneously. We investigated the effects of pellet size distribution and diameter of laser, flux. As a result, this method was found to be able to apply in practical purposes (Kihara, Kawagoe, and Noda ; Chemical Engineering Symposium Series 77, 52-57 (2003)).(3) Development of separation methods of useful substances by reverse micelles: We investigated protein extraction using AOT reverse micelles and found a new method in which extraction efficiency was extremely increased. This method was found to be effective in recovering extracted protein (backward extraction) from the AOT reverse micellar organic phase (Naoe et al ; Biochem. Eng. J., 10, 137-142 (2002)). Employing reverse micelles of sugar esters, we succeeded in extracting proteins with large molecular weight which was difficult to extract in previous method (Naoe et al; Japan Society for Food Engineering, Third Annual Meeting, PF-7 (2002)). Enzyme stability was evaluated by using Lipase-catalyzed hydrolysis (15th International Symposium on Plant Lipids, p.7-8 (2002), Naoe et al; International Congress on Biocatalysis 2002, P056 (2002)). We also investigated lipase-catalyzed esterification in microemulsion-based organogels with gelatin (Nagayama et al; International Congress on Biocatalysis 2002, P038 (2002)). Less

（1）有用的肌肉菌丝体的淹没培养物：我们发现了新型的三核瘤菌丝体，其生长速率五次与我们实验室中的原始物种一样高。这种新型的菌丝体是在标准气泡柱上培养的，以找到中等成分，pH和曝气速率的最佳条件（Kawagoe和Itoh； Nara国家技术学院的研究报告，38，83-86（2002））。此外，我们估计了外部环空运柱中循环速度的悬浮型毛毛菌的悬浮培养基的明显粘度（Kawagoe；日本多相流量学会，年度会议，2002年，B118（B118（2002）））。在标准气泡柱生物反应器中（kawagoe。;未发表）中，还发现了对充气率，pH和中等成分的最佳条件。从激光传递和脉冲宽度中，简单地估算了菌丝体颗粒的浓度和大小。我们研究了助力物的尺寸分布和直径的助焊剂的影响。结果，发现该方法能够用于实际目的（Kihara，Kawagoe和Noda；化学工程研讨会系列77，52-57（2003））。（3）反向胶束开发有用物质的分离方法的开发：我们使用AOOT反向胶束进行了提取的蛋白质提取，并发现了一种新的方法，该方法促进了新的方法，这是极其提高效率的效率。发现该方法可有效从AOT反向胶束有机相中恢复提取的蛋白质（向后提取）（Naoe等；Biochem。Eng。J.，10，137-142（2002））。使用反向糖酯的反向胶束，我们成功地提取了具有较大分子量的蛋白质，这在先前的方法中很难提取（Naoe等；日本食品工程学会，第三届年度会议，PF-7（2002））。使用脂肪酶催化的水解评估酶稳定性（第15届国际植物脂质研讨会，第7-8页（2002），Naoe等；国际生物催化大会2002，P056（2002））。我们还研究了用明胶的基于微乳液的有机凝胶中脂肪酶催化的酯化（Nagayama等；国际生物催化大会，2002年，P038（2002））。较少的