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High Speed and Large Scale Submerged Culture of Useful Mushroom Mycelium in Bubble Column Bioreactors

有用蘑菇菌丝体在泡罩生物反应器中的高速大规模深层培养

基本信息

批准号：
13555229
负责人：
KAWAGOE Mikio
金额：
$ 4.86万
依托单位：
Nara National College of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

(1) Submerged culture of useful mushroom mycelium: We found new type of Tricoloma matsutake mycelium of which growth rate was five time as high as that of original species in our laboratory. This new type of mycelium was cultured in a standard bubble column to find the optimum conditions on medium composition, pH, and aeration rate (Kawagoe and Itoh ; Research Reports of Nara National College of Technology, 38, 83-86 (2002)). Also, we estimated apparent viscosity of suspended medium of T. matsutake mycelium from the circulation velocity in an external-loop airlift column (Kawagoe ; The Japanese society for multiphase flow, Annual Meeting 2002, B118 (2002)). Optimum conditions on aeration rate, pH, and medium compositions were also found for submerged culture of Agaricus blazei in a standard bubble column bioreactor (Kawagoe. ; unpublished).(2) Measurement of mycelium pellets concentration by laser transmission method : We developed a novel measurement method of mycelium pellets concent … More ration by laser transmission. From the laser transmission and pulse width, concentration and size of mycelium pellets were estimated simultaneously. We investigated the effects of pellet size distribution and diameter of laser, flux. As a result, this method was found to be able to apply in practical purposes (Kihara, Kawagoe, and Noda ; Chemical Engineering Symposium Series 77, 52-57 (2003)).(3) Development of separation methods of useful substances by reverse micelles: We investigated protein extraction using AOT reverse micelles and found a new method in which extraction efficiency was extremely increased. This method was found to be effective in recovering extracted protein (backward extraction) from the AOT reverse micellar organic phase (Naoe et al ; Biochem. Eng. J., 10, 137-142 (2002)). Employing reverse micelles of sugar esters, we succeeded in extracting proteins with large molecular weight which was difficult to extract in previous method (Naoe et al; Japan Society for Food Engineering, Third Annual Meeting, PF-7 (2002)). Enzyme stability was evaluated by using Lipase-catalyzed hydrolysis (15th International Symposium on Plant Lipids, p.7-8 (2002), Naoe et al; International Congress on Biocatalysis 2002, P056 (2002)). We also investigated lipase-catalyzed esterification in microemulsion-based organogels with gelatin (Nagayama et al; International Congress on Biocatalysis 2002, P038 (2002)). Less

(1)蘑菇菌丝体深层培养：我们在实验室发现了一种新的松茸菌丝体，其生长速度是原菌种的5倍。这种新型的菌丝体在标准鼓泡塔中培养，以找到培养基组分、pH和通气量的最佳条件(Kawagoe和Itoh；奈良国立理工学院研究报告，38，83-86(2002))。此外，我们根据外环气升柱中的循环速度估计了松茸菌丝体悬浮介质的表观粘度(Kawagoe；日本多相流协会，2002年年会，B118(2002))。采用标准鼓泡塔生物反应器(Kawagoe)对姬松茸深层培养的通气量、pH和培养基组分进行了优化。(2)激光透射法测定菌丝球浓度：我们提出了一种测量菌丝球浓度…的新方法。更多的定量通过激光传输。根据激光透过率和脉冲宽度，同时估计了菌丝体颗粒的浓度和大小。考察了颗粒大小分布、激光直径、流量等因素对激光性能的影响。因此，这种方法被发现能够应用于实际目的(Kihara，Kawagoe和Noda，化学工程研讨会系列77，52-57(2003))。(3)反胶束分离有用物质的方法的发展：我们研究了利用AOT反胶束提取蛋白质，发现了一种新的方法，极大地提高了提取效率。这种方法被发现在从AOT反胶束有机相中回收提取的蛋白质(反向提取)是有效的(Naoe等人；Biochem)。英语。J.，10,137-142(2002))。利用糖酯的反胶束，我们成功地提取了以前方法难以提取的大分子蛋白质(Naoe等人；日本食品工程学会，第三届年会，PF-7(2002))。用脂肪酶催化的水解法评价酶的稳定性(第15届国际植物脂类研讨会，第7-8页(2002)，Naoe等人；国际生物催化大会，P056(2002))。我们还研究了脂肪酶催化的微乳液有机凝胶与明胶的酯化反应(Nagayama等人；2002年国际生物催化大会，P038(2002))。较少