Study on Submerged Culture of Mushroom Mycelium by Bubble Column Fermentors

气泡柱发酵罐深层培养蘑菇菌丝体的研究

基本信息

批准号：
08555208
负责人：
KAWAGOE Mikio
金额：
$ 4.8万
依托单位：
NARA NATIONAL COLLEGE OF TECHNOLOGY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

This study is composed of two parts ; the study on submerged culture of mushroom mycelium by bubble column bioreactors, and the study on separation of protein by reverse micelles.I.Submerged culture of mushroom mycelium by bubble column bioreactors : We devised a novel type of airlift column with a tapered riser which is anticipated to be suitable to the submerged culture of mushroom mycelium. The hydrodynamics (gas holdup and liquid circulation velocity) and the oxygen transfer rate in the airlift column were investigated empirically and theoretically. Conditions to suspend large spherical pellets, which are often formed for the submerged culture of mushroom mycelium, in the standard bubble column were investigated. Next, submerged culture of mushroom mycelium was carried out using two species of mushroom : one is productipn of L-malic acid by Schizophyllum commune, and the other is large-scale culture of Tricholoma matsutake. The optimum conditions of the submerged culture on medium composition, temperature, and air flow rate were found for each mushroom.2. Extraction of protein by reverse micelles : Since mushroom mycelium produces many useful proteins, extraction of protein by reverse micelles was investigated. We found the minimal and optimal AOT concentration for the extraction of lysozyme using reverse micelles. The minimal AOT concentration of a binary protein mixture containing cytocbrome c and lysozyme was found to be smaller than the sum of the minimal AOT concentration of each protein obtained in single protein systems. The addition of low concentration of guanidium salts into the feed solution as a pretreatment decreased the minimal AOT concentration, increased the forward extraction, and enabled the successful recoverry of the protein from the micellar organic phase, while the trehalose addition reduced the forward extraction. Using non-ionic Span 60 reverse micelles, cytochrome c was successfully extracted at concentration higher than the CMC.

这项研究由两个部分组成。泡泡柱生物反应器对蘑菇菌丝体的浸没培养的研究，以及通过反向胶束分离蛋白质的研究。从经验和理论上研究了空气动力学（气体保持和液体循环速度）和空运柱中的氧转移速率。研究了标准气泡柱中悬挂大型球形颗粒，通常是为蘑菇菌丝体淹没培养的条件。接下来，使用两种蘑菇进行了蘑菇菌丝体的淹没培养物：一种是schizophyllum cumince的L-麦酸的产品，另一个是tricholoma Matsutake的大规模培养。在每个蘑菇中发现了在中等成分，温度和空气流量上淹没培养物的最佳条件。2。通过反向胶束提取蛋白质：由于蘑菇菌丝体产生许多有用的蛋白质，因此研究了反向胶束提取蛋白质。我们发现使用反向胶束提取溶菌酶的最低和最佳AOT浓度。发现含有细胞染料C和溶菌酶的二元蛋白混合物的最小AOT浓度小于在单个蛋白质系统中获得的每种蛋白质的最小AOT浓度的总和。在预处理中，将低浓度的鸟嘌呤盐添加到进料溶液中，从而降低了AOT浓度，增加了正向提取，并使蛋白质从胶束有机相中成功恢复，而海藻糖添加降低了正向提取。使用非离子跨度60反向胶束，在高于CMC的浓度下成功提取细胞色素C。

项目成果

期刊论文数量（100）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

M.Kawagoe, and T.Maeoka: ""Flow Characteristics of an External-Loop Airlift Column with a Tapered Riser"" Kagaku kogaku Ronbunshu. 22. 629-634 (1996)

M.Kawagoe 和 T.Maeoka：“带有锥形立管的外环空运塔的流动特性”Kagaku kogaku Ronbunshu。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

M.Kawagoe, Y.Nakamura, and H.Noda: ""Cultivation of Matsutake Mycelium in Bubble Column Bioreactors"" Chemical Engineering Symposium Series 57 <Horizon of Biochemical Engineering>. 109-112 (1997)

M.Kawagoe、Y.Nakamura 和 H.Noda：““鼓泡塔生物反应器中松茸菌丝体的培养””化学工程研讨会系列 57 <生化工程的视野>。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

K.Naoe, T.Kai, M.Kawagoe, and M.Imai: ""Extraction of flexibly structured protein in AOT reverse micelles : the flexible structure of protein is the dominant factor for its incorporation into reverse micelles"" Biochem.Eng.J.3. 79-85 (1999)

K.Naoe、T.Kai、M.Kawagoe 和 M.Imai：“AOT 反胶束中柔性结构蛋白质的提取：蛋白质的柔性结构是其掺入反胶束的主导因素”Biochem.Eng。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

K.Naoe,O.Ura,M.Hattori,M.Kawagoe and M.Imai: "Protein Extraction in reverse micelles of Span 60" In“Advances in Bioseparation Engineerin 1997",(Ed.by The Special Study Group for Bioseparation Engineering of SCEJ),(1998). 91-96 (1998)

K.Naoe、O.Ura、M.Hattori、M.Kawagoe 和 M.Imai：“Span 60 反胶束中的蛋白质提取”，“Advances in Bioseparation Engineeringin 1997”，（由生物分离工程特别研究组编辑） SCEJ），（1998）91-96（1998）。