Development of a Multichannel Quartz Crystal Microbalance with High Sensitivity for Simultaneous Detections of Multi-substances and Its Application as Biosensors

高灵敏度多物质同时检测多通道石英晶体微天平的研制及其生物传感器应用

• 批准号: 13555235
• 负责人: HATOZAKI Osamu
• 金额: $ 8.19万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13555235/
• 关键词: Quartz Crystal Microbalance; Multichannel Sensors; Simultaneous Detection; Antigen-Antibody Reactions; Magnetic Microbeads; 水晶振動子電極; 電気化学発光; 微小センサー; 抗原抗体反応

In this research project, development of a highly sensitive multichannel quarts crystal microbalance (MQCM) was carried out. Small QCM electrodes, the diameter of which was less than 2mm, were fabricated on a single quartz plate (22mmx22mm). That each QCM was able to operate as an independent sensor device without interference from other QCM electrodes on the same MQCM was successfully confirmed in both air and liquid phases. Ih addition, high sensitivity of QCM technique was maintained by increasing resonant frequency by decreasing the thickness of a quartz crystal plate. The major subject of this research project was to develop a highly sensitive biosensor based on MQCM technology for quantitative, simultaneous detections of several substances. To reach this goal, different specficity should be given to each of QCM electrodes on a MQCM. Monoclonal antibodies, anti-HSA and anti-BSA, were introduced to the surface of channel 1 and channel 2, respectively, on a 4-channel MQCM. When the antibody-modified MQCM was contacted with a solution containing both HSA and BSA, the resonant frequency of each channel decreased, the magnitude of which was linearly dependent on a concentration of the corresponding antigen. However, at a high antigen concentration, nonspecific adsorption of antigens was observed. Magnetic microbeads, the surface of which was previously modified with, for example, various antibodies, were considered as a technique to give specificity to QCM electrode surface. Using these magnetic microbeads which were readily collected on a QCM electrode surface with a small magnet and removed from the surface after measurements, repetitive use of MQCM was expected, decreasing the cost of the measurements. We expect highly sensitive sensors based on MQCM technology developed in this research project will be useful for detections of many diseases including cancers and tumors at their very early stages.

本研究项目开展了高灵敏度多通道石英晶体微天平的研制工作。在一块(22 mm×22 mm)石英板上制作了直径小于2 mm的QCM小电极。每个QCM能够作为独立的传感器设备运行，不受同一MQCM上其他QCM电极的干扰，这一点在空气和液体中都得到了成功的证实。此外，通过减小石英晶片的厚度来提高谐振频率，从而保持了QCM技术的高灵敏度。本研究项目的主要内容是开发一种基于MQCM技术的高灵敏度生物传感器，用于定量、同时检测几种物质。为了达到这一目标，应该为MQCM上的每个QCM电极赋予不同的特性。在4通道MQCM上，将抗HSA和抗BSA的单抗分别引入通道1和通道2的表面。当抗体修饰的MQCM与同时含有HSA和BSA的溶液接触时，每个通道的共振频率降低，其幅度与相应抗原的浓度线性相关。然而，在较高的抗原浓度下，观察到抗原的非特异性吸附。磁性微珠的表面以前用各种抗体修饰，被认为是一种赋予QCM电极表面特异性的技术。利用这些磁性微珠，这些磁性微珠可以很容易地收集在QCM电极表面，并在测量后从表面移除，有望重复使用MQCM，从而降低测量成本。我们期望本研究项目中开发的基于MQCM技术的高灵敏度传感器将用于许多疾病的检测，包括癌症和肿瘤的早期阶段。