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Development of a Portable Incubation Chamber for Microalgae using Light Emitting Diodes

使用发光二极管开发便携式微藻孵化室

基本信息

批准号：
13556029
负责人：
FUKUYO Yasuwo
金额：
$ 4.93万
依托单位：
THE UNIVERSITY OF TOKYO
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

It is fundamental to establish clonal cultures from various part of world for study on biological, ecophysiological and biochemical features of harmful marine microalgae which are thought to have spread globally. But the culture establishment is sometimes difficult, because areas affected by the harmful microalgae are often under-developed coastal area and far from sophisticated laboratories equipped with culturing facilities.This study aimed to develop a Portable incubation chamber furnishing temperature and light control devices. The light resource is obtained by Light Emitting Diodes (LEDs) and it poses many advantages on its smaller size, safety and low electric consumption. Various dinoflagellates and diatoms shows smooth growth comparable to natural light under red, green and blue lights from the LEDs. A dmoflagellate Heterocapsa cocularisquama grows well under red light, and Alexandrium spp under blue light. Different species shows small variation on optimum light condition (strength and color) for the highest growth rate, and it may come from difference of adaptability of species to lights from LEDs. Optimal spectra output in the region of photosynthetic pigment absorption should be found to use the very narrow spectrum output of LEDs. While the temperature could be regulated at a desired temperature between 10 and 30 degree in Celsius using a Peltiert module with a thermo controller, necessary electrical power is high and continuous use of the regulator may cause problem on the capacity of the chamber and maintenance cost of a battery. From these result a chamber (30x20x20 cm) was successfully made and used with good results for transfer of microalgae from the Philippines and Thailand to Japan. This portable incubation chamber enables the maintenance of living cells during handling and field work so that more cultures of harmful microalgae can be established from much wider location.

建立世界各地的克隆培养体系是研究有害海洋微藻的生物学、生态生理和生化特性的基础，这些微藻被认为是全球性传播的。但有时培养设施很难建立，因为受有害微藻影响的地区往往是欠发达的沿海地区，而且远离配备有培养设施的先进实验室。本研究旨在开发一种便携式孵化室，提供温光控制装置。光源采用发光二极管(LED)，具有体积小、安全、耗电量低等优点。各种甲藻和硅藻在LED的红、绿和蓝光下显示出与自然光相当的平滑生长。异鞭毛藻Cocularisquama在红光下生长良好，亚历山大藻在蓝光下生长良好。不同树种对最高生长速率的最适光照条件(强度和颜色)差异不大，这可能是由于树种对LED光的适应能力不同所致。在光合色素吸收区域的最佳光谱输出应该使用LED非常窄的光谱输出。虽然使用带有温度控制器的Peltiert模块可以将温度调节在10到30摄氏度之间的所需温度，但必要的电力很高，连续使用调节器可能会导致电池室容量和电池维护成本的问题。根据这些结果，成功地制作了一个30×20×20厘米的小室，并将其用于从菲律宾和泰国向日本转移微藻，取得了良好的效果。这种便携式孵化箱能够在搬运和野外工作期间维护活细胞，从而可以从更广泛的地点建立更多有害微藻的培养。

项目成果

期刊论文数量（44）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Yasuwo Fukuyo, Masaaki Kodama, and Takehiko Ogata: "Review : Toxic Microalgae"Kaiyo Monthly. 33(10). 685-688 (2001)

Yasuwo Fukuyo、Masaaki Kodama 和 Takehiko Ogata：“评论：有毒微藻”Kaiyo 月刊。

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Shigeru Itakura: "The seasonal occurrence of Alexandrium tamarense (Dinophyceae) vegetative cells in Hiroshima Bay, Japan"Fisheries Science. 66. 77-89 (2002)

Shigeru Itakura：“日本广岛湾塔玛亚历山大藻（甲藻纲）营养细胞的季节性出现”渔业科学。

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Makoto Yoshida: "Development of a portable incubation chamber for phytoplankton"Abstracts of the 10th International Conference on Harmful Microalgae. 310 (2002)

吉田诚：“浮游植物便携式孵化室的开发”第十届有害微藻国际会议摘要。

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Mitsunori Iwataki, Haruyoshi Takayama, Kazumi Matsuoka and Yasuwo Fukuyo: "Heterocapsa lanceolata sp. nov. and Heterocapsa horiguchit sp. nov. (Peridiniales, Dinophyceae), two new marine dinoflagellates from coastal Japan"Phycologia. 41(5). 470-479 (2002)

Mitsunori Iwataki、Haruyoshi Takayama、Kazumi Matsuoka 和 Yasuwo Fukuyo：“Heterocapsa lanceolata sp. nov. 和 Heterocapsa horiguchit sp. nov.（多甲藻目，甲藻科），来自日本沿海的两种新海洋甲藻”Phycologia。