Assay Development of urinary 3-hydroxproline derived from basement membrane collagen for cancer screening

用于癌症筛查的源自基底膜胶原蛋白的尿 3-羟脯氨酸测定方法的开发

• 批准号: 13557034
• 负责人: OKAZAKI Isao
• 金额: $ 5.44万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13557034/
• 关键词: cancer screening; 3 - hydroxyproline; urinary excretion; type IV collagen; community health; 地域保健

The Welfare and Health Law for The Elderly in Japan has requested the cancer screening for stomach, lung, breast, uterus and colon. The budget to support the law, however, is not included in the Japan Government, neither in the Prefectural Government. The local governments for citizens are wondering about cancer screening service by public support. We propose all purpose cancer screening system which means the target organ is not specialized. Okazaki et al. published an assay method as ELISA kit for measuring urinary 3-Hydroxyproline. The first year (2001) we succeeded in synthesis of Cys (3Hyp - 4Hyp - Gly)3 and Cys (Prol - 4Hyp - Gly)3 as antigen to get the antibody in rabbits. The latter peptide was also administered to rule out the specific antibody against 3Hyp - containing peptide. We succeeded again to establish a new ELISA kit for urinary 3 - hyp - containing peptide. We applied this new method for 199 healthy persons and 10 cancer patients. The values of urinary 3Hyp content measured by conventional method and ELISA kit method were parallel. The big difference was seen between the value in healthy persons and that in cancer patients.

日本《老年福利与健康法》要求对胃癌、肺癌、乳腺癌、子宫癌和结肠癌进行筛查。然而，支持这项法律的预算不包括在日本政府，也不包括在县政府。对于公众支持的癌症筛查服务，当地政府对此感到疑惑。我们提出了通用的癌症筛查系统，即靶器官不是专门的。Okazaki et al.发布了一种测定尿中3-羟基脯氨酸的酶联免疫吸附试验试剂盒。第一年(2001)，我们成功地合成了Cys(3Hyp-4Hyp-Gly)3和Cys(Prol-4Hyp-Gly)3作为抗原，在兔体内获得了抗体。后一种多肽也被用来排除3Hyp多肽的特异性抗体。我们再次成功地建立了一种新的尿液3-羟基肽酶联免疫吸附试验试剂盒。我们将这种新方法应用于199名健康人和10名癌症患者。常规法和酶联免疫吸附试验试剂盒法测定的尿3Hyp含量基本一致。健康人和癌症患者之间的值有很大差异。

项目成果

松山重雄, 丸田明枝, ほか: "尿3-Hydroxyprolineによるがん検診への応用"Connective Tissue. 33(2). 135-135 (2001)

Shigeo Matsuyama、Akie Maruta 等人：“尿液 3-羟脯氨酸在癌症筛查中的应用”，Connective Tissue 33(2)。

Watanabe T, et al.: "Neural stem/progenitor cells appear in the recovery from liver fibrosis"Hepatology. 36(4). 248-248 (2002)

Watanabe T 等人：“肝纤维化恢复过程中出现神经干/祖细胞”肝病学。

Inagaki Y, Nemoto T, Watanabe T, Okazaki I: "Interferon a antagonizes transforming growth factor b-Stimulated collagen gene transcription and suppresses promoter activation"The 53rd Annual Meeting of The American Association for The Study of Liver Disease

Inagaki Y、Nemoto T、Watanabe T、Okazaki I：“干扰素 a 拮抗转化生长因子 b-刺激胶原蛋白基因转录并抑制启动子激活”美国肝病研究协会第 53 届年会

岡崎 勳, 新岡 真希: "消化器ナビゲーター (滝川 一 編)"細胞外マトリックス. 2 (2002)

冈崎淳、新冈真纪：“胃肠导航器（泷川肇编辑）”细胞外基质 2（2002 年）。

岡崎 勲, 新岡 真希: "消化器ナビゲーター(滝川 一編)"細胞外マトリックス. 2 (2002)

冈崎功、新冈真希：“胃肠导航器（泷川肇编辑）”细胞外基质 2（2002 年）。