Detection of sleep regulating genes by DNA microarray to apply for clinical anesthesia

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Sleep and anesthesia have common characteristics, e.g. loss of consciousness. In deed, some hypnotic agents like benzodiazepines were used in the area of clinical anesthesia. Physiological sleep also has important functions including protection of brain or enhancement of immune system. Recently, many endogenous substances regulating sleep (sleep factors) were studied. Particularly, interleukin-1, growth hormone releasing hormone and prostaglandin D2 are very notable. However, it is still unknown what sleep factor is the most important. Furthermore, relationship between sleep and anesthesia is not studied so well. Finally, there is almost no study to apply the endogenous sleep factors for clinical anesthesia. In the present study, we detected main sleep related genes by DNA microarray. We found that expressions of some genes related to endogenous sleep factors, e.g. interleukin-1 and growth hormone releasing hormone, were enhances after 8-h sleep deprivation in the rat whole brain. Then, we found that sleep deprivation make the anesthetic potency strong in rats. In conclusion, some sleep factors or their related substances may be used as anesthetic supplements.