Development of Hybrid Type Dental Implant with Periodontal Liquament

牙周液混合型种植牙的研制

• 批准号: 13557165
• 负责人: TSUTSUMI Sadami
• 金额: $ 7.62万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13557165/
• 关键词: Implant; Periodontal ligament; Regenerate; Hybrid; Collagen; ハイドロキシアパタイト; セメント質; 人工歯根; 歯根膜再生; コラーゲン固定化; 三次元培養; チタン-EVA接着; コラーゲンスポンジ; 回転播種法

In order to require the functional recovery of the dental implant, a trial of regenerating PDL around hybrid implant. Our hybrid implant was prepared as follows. First, the titanium implant was oxidized by 30% hydrogen peroxide at 60 ℃ for 24 h. Second, poly(ethylene-co-vinyl alcohol)(EVA) was coated onto the implant and ozone treatment was carried out for introduction of carboxyl groups. Third, the collagen sponge was immobilized around the implant by freeze-drying method. Last, PDL cells were seeded onto the implant and implanted in dog's alveolar bone. The cells can be seeded uniformly around the implant by this method. After 3 months of implantation, the implants and their surrounding tissue were recovered and the histological specimens were prepared. The collagen fiber around implant was seemed to be vertically to the alveolar bone similar to the one around natural tooth. The results suggested that the novel hybrid implant has a potentiality to regenerate periodontal tissues containing of PDL and cementum.

为满足种植体功能恢复的需要，进行了混合种植体周围牙周膜再生的实验研究。我们的混合植入物制备如下。首先，用30%过氧化氢在60 ℃下氧化24 h。其次，将聚（乙烯-co-乙烯醇）（伊娃）涂覆到植入物上，并进行臭氧处理以引入羧基。第三，采用冷冻干燥法将胶原海绵固定在种植体周围。最后，将PDL细胞接种到种植体上并植入狗的牙槽骨中。通过这种方法可以将细胞均匀地接种在植入物周围。植入3个月后，取出植入物及其周围组织，制备组织学标本。种植体周围的胶原纤维与牙槽骨垂直，与天然牙周围的胶原纤维相似。实验结果表明，该复合种植体具有促进牙周组织再生的潜力。

项目成果

Matsumura, K, Tsutusmi, S.et al.: "Adhesion between ethlene-vinyl alcohol copolymer and titanium"Technology on Adhesion and Sealing. 46. 501-505 (2002)

Matsumura, K, Tsutusmi, S.et al.：“乙烯-乙烯醇共聚物与钛之间的粘合”粘合和密封技术。

Matsumura, K., Hyon, S.-H., Tsutsumi, S.et al.: "Surface modification of poly(ethylene-co-vinyl alcohol)(EVA) : Hydroxyapatite immobilization and control of periodontal ligament cells differentiation"Biomaterials. 25. 4817-4824 (2004)

Matsumura, K.、Hyon, S.-H.、Ttsutsumi, S.等人：“聚乙烯-乙烯醇共聚物 (EVA) 的表面改性：羟基磷灰石固定和牙周膜细胞分化的控制”生物材料。

Tsutsumi, S.: "Biomaterials for Dentistry"Biomaterials. 235-252 (2002)

Tsutsumi, S.：“牙科生物材料”生物材料。

Peng, C., Tsutsumi, S.et al.: "Morphologic Study and Syntheses of Type 1 Collagen and Fibronection of Human Periodontal Ligamentt Cells Cultured on Poly(ethylene-co-vinyl alcohol)(EVA) with Collagen Immobilization"Journal of Biomedical Research. 54. 241-2

Peng, C., Tsutsumi, S.等人：“胶原固定化聚乙烯-乙烯醇 (EVA) 上培养的人牙周膜细胞的 1 型胶原的形态学研究和合成及纤维连接”生物医学杂志

Matsumura, K., Tsutsumi, S.et al.: "Trials toward the regeneration of periodontal tissue around titanium implant"The Sixth Annual International Conference and Exposition for the Tissue Engineering Society International (Abstract). (2003)

Matsumura, K.、Ttsutsumi, S.等人：“钛种植体周围牙周组织再生的试验”国际组织工程学会第六届年度国际会议暨博览会（摘要）。