Development of Nanobiore actor for Remediation of Foods and Environments

开发用于修复食品和环境的纳米生物演员

• 批准号: 13558072
• 负责人: FUJII Tomoyuki
• 金额: $ 4.42万
• 依托单位: Niigata University of Pharmacy and Applied Life Sciences (2002)The University of Tokyo (2001)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13558072/
• 关键词: nanospace; fractal; nanoporous silica membrane; laccase; endocrine disrupting chemical; supercritical carbon dioxide; lipase; critical phenomena; silica membrane

A thin nanoporous silica membrane was prepared by the two-step sol-gel method for the purpose that the generation of an aggregate having the higher fractal dimension was suppressed in the intermediate process. It succeeded in the preparation of the thin silica film with nanospace of about 3.3nm. The model reaction in which sea sand was made to adsorb the alkylphenol was carried out, and it was found that adsorbing nonylphenol and octylphenol and bisphenol A are quickly decomposed by laccase. The decomposition of an artificial estrogen also confirmed being possible. Log P, which was an useful parameter when we evaluate the solvent property of supercritical carbon dioxide, was estimated. As the pressure of carbon dioxide was increase on the pressure of 3.0 11.8 MPa, log P tended to increase from 0.9 to 2.0. It was shown that the polarity had appeared in carbon dioxide under the supercritical state by the pressure, and that the polarity became small with the increase in the pressure. On the esterification reaction between stearic acid and ethanol using lipase, the reaction rate rose with the increase in the pressure, and it agreed with the increase tendency of log P approximately. It was shown from these results that the solvent property as a bulk had to be considered in addition to the interaction between solute and carbon dioxide, enzyme surface, wall surface, respectively, when it examined the enzyme reaction in nanospace by using supercritical carbon dioxide as a solvent.

采用两步溶胶-凝胶法制备了一种纳米多孔二氧化硅薄膜，以抑制中间过程中分形维数较高的聚集体的生成。成功地制备了纳米间距约为3.3nm的二氧化硅薄膜。用海砂吸附烷基酚进行模型反应，发现吸附的壬基酚、辛基酚和双酚A被漆酶快速分解。人工雌激素的分解也被证实是可能的。对logp进行了估计，它是评价超临界二氧化碳溶剂性质的一个有用参数。在3.0 ~ 11.8 MPa时，随着二氧化碳压力的增加，log P有从0.9增大到2.0的趋势。结果表明，超临界状态下的二氧化碳在压力作用下出现极性，且极性随压力的增大而变小。在脂肪酶作用下硬脂酸与乙醇酯化反应中，反应速率随压力的增大而增大，近似符合log P的增大趋势。这些结果表明，在纳米空间中以超临界二氧化碳为溶剂考察酶反应时，除了溶质与二氧化碳、酶表面、壁表面的相互作用外，还必须考虑溶剂的整体性质。

项目成果

T.Tanaka, et al.: "Treatment of Model Soils Contaminated with Phenolic Endocrine Disrupting Chemicals by Laccase from Trametes s in a Rotating Reactor"Journal of Bioscience and Bioengineering. 92-4. 312-316 (2001)

T.Tanaka等人：“旋转反应器中来自栓菌的漆酶对受酚类内分泌干扰化学品污染的模型土壤的处理”生物科学与生物工程杂志。

T.Tanaka, et al.: "Treatment of Model Soils Contaminated with Phenolic Endocrine Disrupting Chemicals by Laccase from Trametes sp. a Rotating Reactor"Journal of Bioscience and Bioengineering. 92(4). 312-316 (2001)

T.Tanaka等人：“通过旋转反应器来自栓菌属的漆酶处理受酚类内分泌干扰化学品污染的模型土壤”生物科学与生物工程杂志。

T.Fujii, et al.: "The Sol-Gel Preparation and Characterization of Nanoporous Silica Membrane with Controlled Pore Size"Journal of Membrane Science. 187-1.2. 171-180 (2001)

T.Fujii 等人：“孔径受控的纳米多孔二氧化硅膜的溶胶-凝胶制备和表征”膜科学杂志。

T.Tanaka, et al.: "Treatment of Model Soils Contaminated with Phenolic Endocrine Disrupting Chemicals by Laccase from Tramentes sp. in a Rotating Reactor"Journal of Bioscience and Bioengineering. 92(4). 312-316 (2001)

T.Tanaka 等人：“在旋转反应器中通过来自 Tramentes sp. 的漆酶处理受酚类内分泌干扰化学品污染的模型土壤”生物科学与生物工程杂志。

H.Nakaya, et al.: "Lipase-Catalyzed Esterification of Stearic Acid with Ethanol, and Hydrolysis of Ethyl Stearate, Near the Critical Point in Supercritical Carbon Dioxide"Journal of American Oil Chemical Society. 79-1. 23-27 (2002)

H.Nakaya等人：“脂肪酶催化的硬脂酸与乙醇的酯化和硬脂酸乙酯的水解，接近超临界二氧化碳的临界点”美国石油化学学会杂志。