Development of Cryopreservation Technique and Its Diffusion in South-east Asia for Conservation of Tropical Plant Germplasms Useful for Search for Bioactive Metabolites.

低温保存技术的发展及其在东南亚的传播，用于保护热带植物种质，有助于寻找生物活性代谢物。

基本信息

批准号：
14406031
负责人：
MIYAMOTO Kazuhisa
金额：
$ 9.47万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

In this research, we investigated the cryopreservation techniques generally applicable to tropical germplasms of photosynthetic organisms such as higher plants and microalgae useful for searching novel bioactive metabolites in Pharmaceutical Science field. We also attempted to diffuse these techniques in South-east Asian countries in which ecosystem in rainforest is rapidly destructed by environmental pollution many precious species of higher plants and microalgae are on the way to extinction.As a fundamental research using plant root and green alga, we found a phytohormone, abscisic acid(ABA), could induce tolerance to dehydration and then freezing. ABA-pretreatment increased the survival rates in these materials after freezing in liquid nitrogen. We applied this pretreatment to several plants and algae and succeeded their cryopreservation.We attempted to search novel bioactive metabolites in microalgae in those areas under cooperation with foreign researchers. Several metabolites showing strong growth inhibitory activities and a biosurfactant could be isolated from freshwater cyanobacteria isolated in Thailand and Indonesia.Finally, we made efforts to diffuse the developed cryopreservation techniques in Thailand and Indonesia as generally applicable ones for conservation of useful tropical plant and algal germplasms. We visited these countries several times and gave lectures and technical workshops to encourage the consciousness for importance of tropical germplasms and increase the individual techniques of cryopreservation.

在这项研究中，我们研究了通常适用于光合生物的热带生殖的冷冻保存技术，例如较高植物和微藻类，可用于搜索药物科学领域中的新型生物活性代谢产物。我们还试图在东南亚国家扩散这些技术，在东亚国家中，雨林中的生态系统迅速被环境污染摧毁了许多珍贵的植物和微藻的珍贵物种，正处于灭绝状态。由于使用植物根和绿色藻类的基本研究，我们发现了植物根和绿色藻类的基础研究。液氮冷冻后，ABA - 预处理提高了这些材料的存活率。我们将这种预处理应用于几种植物和藻类，并继承了他们的冷冻保存。我们试图在与外国研究人员合作的那些地区，在微藻中搜索新型的生物活性代谢产物。几种表现出强烈生长抑制活性的代谢产物，可以从泰国和印度尼西亚分离的淡水蓝细菌中分离出强大的生长抑制作用，并且在本文中，我们努力弥补泰国和印度尼西亚开发的冷冻保存技术，通常适用于保存有用的热带植物和Algal Gertlagalsms。我们多次访问了这些国家，并进行了讲座和技术研讨会，以鼓励意识到热带种系的重要性并增加了冷冻保存的个人技术。