Enhancement of Allergen-induced Eosinophilic Inflammation and Cytokine/Chemokine Expression Expression in the Murine Lungs by Asian Sand Dust

亚洲沙尘增强小鼠肺中过敏原诱导的嗜酸性粒细胞炎症和细胞因子/趋化因子表达

• 批准号: 14580572
• 负责人: ICHINOSE Takamichi
• 金额: $ 1.79万
• 依托单位: Oita University of Nursing and Health Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14580572/
• 关键词: Asian dust; Bronchial astma; It injection; Murin Lung; Eosinophils; Goblet cells; IgE production; IgG1 production; アレルギー; 肺; 毒性; 炎症; サイトカイン; ケモカイン; マウス; IL-5; 気道炎症; 抗体産生

Background: Asian sand dust (Kosa) contains irritant chemical species, such as SO^<2->_4. Although Kosa causes adverse respiratory health effects, there is no experimental study showing the effect of Kosa on allergic asthma. This study was undertaken to clarify the effects of Kosa on ovalbumin (OVA)-induced eosinophilic inflammation in the murine lung. Methods: ICR mice were administered intratracheally with saline; Kosa alone (sample from Shapotou desert); Kosa plus SO^<2->_4 (Kosa -SO_4); Natural Asian dust (NAD) from Beijing; OVA, OVA + Kosa; OVA + Kosa -SO_4; or OVA + NAD. Results: The three sand dusts, especially NAD, aggravated the eosinophilc airway inflammation accompanied by OVA-induced goblet cell proliferation. The additive effect of SO^<2->_4 was not observed. OVA which was induced by sand dusts suppressed the expression of interleukin (IL)-1 2 and interferon (IFN)-γ in bronchoalveolar lavage fluids (BALF). In contrast, these sand dusts, especially NAD, synergistically increased IL-5, eotaxin, and monocyte chemotactic protein (MCP)-1 in BALF and lung tissue related to OVA. NAD caused the adjuvant effects to IgG1 and IgE production. Conclusions: These results suggest that sand dusts enhance OVA-induced eosinophilic inflammation in the lungs. It is hypothesized that this enhancement is induced by the synergistic effect between IL-S and eotaxin and MCP-1 via the suppression of IL-12 and IFN-γ

背景：亚洲沙尘(KOSA)含有刺激性化学物质，如SO^&lt；2-gt；_4。虽然KOSA会对呼吸道健康造成不良影响，但尚无实验研究表明KOSA对过敏性哮喘的影响。本研究旨在阐明KOSA对卵清蛋白(OVA)诱导的小鼠肺嗜酸性粒细胞炎症的影响。方法：ICR小鼠气管内注射生理盐水、KOSA(沙坡头沙漠标本)、KOSA+SO4(KOSA-SO4)、北京天然亚洲粉尘(NAD)、OVA、OVA+KOSA、OVA+COSA-SO4或OVA+NAD。结果：三种沙尘均可加重OVA诱导的杯状细胞增殖所致的嗜酸性粒细胞炎症，其中以NAD最为明显。未观察到SO^&lt；2-&gt；4的相加效应。沙尘诱发的卵子可抑制小鼠肺泡灌洗液中白细胞介素1 2和干扰素γ的表达。相反，这些沙尘，尤其是NAD，协同增加了BALF和与OVA相关的肺组织中IL-5、嗜酸性粒细胞趋化因子和单核细胞趋化蛋白(MCP)-1的含量。NAD对IgG1和IgE的产生有佐剂作用。结论：这些结果提示沙尘可增强OVA诱导的肺部嗜酸性炎症。推测这种增强作用是由于IL-S通过抑制IL-12和干扰素γ而与嗜酸性粒细胞趋化因子和单核细胞趋化蛋白-1协同作用所致。