Experimental study on IgE non-dependent asthma like symptom and mice strain differences

IgE非依赖性哮喘样症状及小鼠品系差异的实验研究

• 批准号: 08680588
• 负责人: ICHINOSE Takamichi
• 金额: $ 1.47万
• 依托单位: National Institute for Environmental Studies
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08680588/
• 关键词: Diesel exhaust particls; Bronchial asthma; It injection; Murin strain difference; Eosinophils; Goblet cells; IgE production; IgG1 production; ディーゼル排気微粒子(DEP); 好酸球浸潤; 粘液産生細胞数; マウスの系統差; IgE抗体価; IgG1抗体価; 気管内投与

To clarify the relationship between the manifestations of allergic airway inflammation modulated by diesel exhaust particles (DEP) and immunoglobulin production in response to an antigen, airway inflammation characterized by the infliltration of eosinophils, goblet cell proliferation, and antigen-specific immunoglobulin (Ig) production was investigated in five strains of mice after immunization with ovalbumin (OA). Mice were injected intratracheally with OA (1mug) or OA (1mug) +DEP (50mug) four times at 3-week intervals. The order of antigen-specific IgG1 production in plasma of mouse strains treated with OA alone was CBA/2N<BDF/1<C57BL/6<ICR<C3H/He. The adjuvant effect of DEP on IgG1 production was observed in CBA/2N,BDF/1, ICR,and C57BL/6 mice. The immune activity in BDF/1 mice was significantly elevated (p<0.01>). The OA-specific IgE in plasma was unaffected by antigen challenge with or without DEP in any strain. The degree of eosinophilic inflammation and goblet cell proliferation in the airway induced by OA alone or OA+DEP corresponded well to the antigen-specific IgG1 production. These results suggest that the manifestations of allergic airway inflammation modulated by DEP were closely related to the immunoglobulin production response to OA,especially with regard to enhanced IgG1 production.

为了阐明柴油机尾气颗粒物（DEP）调节的过敏性气道炎症的表现与免疫球蛋白产生之间的关系，研究了5种小鼠在卵清蛋白（OA）免疫后的气道炎症，其特征在于嗜酸性粒细胞的浸润、杯状细胞的增殖和抗原特异性免疫球蛋白（IG）的产生。每隔3周给小鼠气管内注射OA（1 μ g）或OA（1 μ g）+DEP（50 μ g）4次。单用OA处理的小鼠品系血浆中抗原特异性IgG 1产生的顺序为CBA/2N<BDF/1<C57 BL/6<ICR<C3 H/He。在CBA/2N、BDF/1、ICR和C57 BL/6小鼠中观察到DEP对IgG 1产生的佐剂作用。BDF/1小鼠的免疫活性显著提高（p<0.01>）。血浆中OA特异性IgE不受任何菌株中有或没有DEP的抗原攻击的影响。OA单独或OA+DEP诱导的气道中嗜酸性粒细胞炎症和杯状细胞增殖的程度与抗原特异性IgG 1的产生很好地对应。这些结果表明，DEP调制的过敏性气道炎症的表现密切相关的免疫球蛋白生产响应OA，特别是在增强IgG 1的生产。

项目成果

Takamichi Ichinose: "Murine Strain differences in allergic airway inflammation and immunoglobu production by a combination of antigen and diesel exhaust particles" Toxicology. 122. 183-192 (1997)

Takamichi Ichinose：“小鼠品系在过敏性气道炎症和抗原与柴油机尾气颗粒组合产生免疫球方面的差异”毒理学。

Takamichi Ichinose, Hirohisa Takano, Yuichi Miyabara, Rie Yanagisawa and Masaru Sagai: "Murine strain differences in allergic airway inflammation and immunoglobulin production by a combination of antigen and diesel exhaust particles." Toxicology. 122. 183

Takamichi Ichinose、Hirohisa Takano、Yuichi Miyabara、Rie Yanagisawa 和 Masaru Sagai：“抗原和柴油机尾气颗粒组合导致过敏性气道炎症和免疫球蛋白产生的小鼠品系差异。”

嵯峨井勝,市瀬孝道ら: "ディーゼル排気微粒子(DEP)による呼吸器疾患〜気管支喘息,アレルギー性鼻炎と肺癌〜"

Masaru Sagai、Takamichi Ichise 等人：“柴油机尾气颗粒物 (DEP) 引起的呼吸道疾病 - 支气管哮喘、过敏性鼻炎和肺癌”

Takamichi, Ichinose: "Murine strain differences in allergic airway inflammation and immunog lobuin ploduction by a combination of antigen and diesel exhaust particles" Toxicology. 122. 183-192 (1997)

Takamichi, Ichinose：“抗原和柴油机尾气颗粒组合导致过敏性气道炎症和免疫球蛋白产生的小鼠品系差异”毒理学。

M.Sagai,T.Ichinoseら: "Biological effects of diesel exhaust particles.IV.Pathogenesis of asthma" Free Radical Biol.Med.21,. 199-209 (1996)

M.Sagai、T.Ichinose 等人：“柴油机废气颗粒的生物效应。IV.哮喘的发病机制”Free Radical Biol.Med.21，1996 年。