Experimental study on IgE non-dependent asthma like symptom and mice strain differences

为了阐明柴油机尾气微粒(DEP)调节的过敏性呼吸道炎症表现与抗原免疫球蛋白产生之间的关系，用卵蛋白(OA)免疫5种品系的小鼠，观察了以嗜酸性粒细胞(EOS)炎症、杯状细胞增殖和抗原特异性免疫球蛋白(Ig)产生为特征的气道炎症。小鼠气管内注射OA(1mug)或OA(1mug)+DEP(50mug)，共4次，间隔3周。小鼠血浆中抗原特异性IgG1的产生顺序为CBA/2N&lt；BDF/1&lt；C57BL/6&lt；ICR&lt；C3H/He。在CBA/2N、BDF/1、ICR和C57BL/6小鼠中观察了DEP对IgG1产生的佐剂作用。BDF/1小鼠的免疫活性显著增强(p&lt；0.01&gt；)。无论有无DEP，血浆中OA特异性IgE均不受抗原攻击的影响。OA单独作用或OA+DEP诱导的气道嗜酸性炎症和杯状细胞增殖程度与抗原特异性IgG1的产生有很好的对应关系。这些结果表明，DEP调节的过敏性气道炎症的表现与免疫球蛋白的产生反应密切相关，尤其是与免疫球蛋白的增加有关。

To clarify the relationship between the manifestations of allergic airway inflammation modulated by diesel exhaust particles (DEP) and immunoglobulin production in response to an antigen, airway inflammation characterized by the infliltration of eosinophils, goblet cell proliferation, and antigen-specific immunoglobulin (Ig) production was investigated in five strains of mice after immunization with ovalbumin (OA). Mice were injected intratracheally with OA (1mug) or OA (1mug) +DEP (50mug) four times at 3-week intervals. The order of antigen-specific IgG1 production in plasma of mouse strains treated with OA alone was CBA/2N<BDF/1<C57BL/6<ICR<C3H/He. The adjuvant effect of DEP on IgG1 production was observed in CBA/2N,BDF/1, ICR,and C57BL/6 mice. The immune activity in BDF/1 mice was significantly elevated (p<0.01>). The OA-specific IgE in plasma was unaffected by antigen challenge with or without DEP in any strain. The degree of eosinophilic inflammation and goblet cell proliferation in the airway induced by OA alone or OA+DEP corresponded well to the antigen-specific IgG1 production. These results suggest that the manifestations of allergic airway inflammation modulated by DEP were closely related to the immunoglobulin production response to OA,especially with regard to enhanced IgG1 production.